Restriction map for
pF1KB9723
(Linear) (Six-base) MAPSORT of: KIBB9723.seq Check: 6297 from: 1 to: 1242
KIBB9723.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 420 1242
Size: 420 822
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 1242
Size: 463 779
AflIII A'CryG_T
Cuts at: 0 668 881 1242
Size: 668 213 361
Fragments arranged by size:
668 361 213
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1038 1242
Size: 1038 204
AlwNI CAG_nnn'CTG
Cuts at: 0 624 1098 1242
Size: 624 474 144
Fragments arranged by size:
624 474 144
ApaI G_GGCC'C
Cuts at: 0 85 505 988 1242
Size: 85 420 483 254
Fragments arranged by size:
483 420 254 85
ApoI r'AATT_y
Cuts at: 0 1084 1242
Size: 1084 158
AscI GG'CGCG_CC
Cuts at: 0 629 1242
Size: 629 613
AvaI C'yCGr_G
Cuts at: 0 505 550 724 907 981 1063 1242
Size: 505 45 174 183 74 82 179
Fragments arranged by size:
505 183 179 174 82 74 45
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 12 45 1242
Size: 12 33 1197
Fragments arranged by size:
1197 33 12
BanI G'GyrC_C
Cuts at: 0 141 745 861 1242
Size: 141 604 116 381
Fragments arranged by size:
604 381 141 116
BanII G_rGCy'C
Cuts at: 0 85 112 505 833 904 988 1061 1242
Size: 85 27 393 328 71 84 73 181
Fragments arranged by size:
393 328 181 85 84 73 71 27
BbeI G_GCGC'C
Cuts at: 0 145 865 1242
Size: 145 720 377
Fragments arranged by size:
720 377 145
BciVI GTATCCnnnnn_n'
Cuts at: 0 13 211 1242
Size: 13 198 1031
Fragments arranged by size:
1031 198 13
BglI GCCn_nnn'nGGC
Cuts at: 0 420 509 1031 1242
Size: 420 89 522 211
Fragments arranged by size:
522 420 211 89
BlpI GC'TnA_GC
Cuts at: 0 106 479 1242
Size: 106 373 763
Fragments arranged by size:
763 373 106
Bme1580I G_kGCm'C
Cuts at: 0 85 505 988 1242
Size: 85 420 483 254
Fragments arranged by size:
483 420 254 85
BmgBI CAC'GTC
Cuts at: 0 671 1239 1242
Size: 671 568 3
Fragments arranged by size:
671 568 3
BmrI ACTGGGnnnn_n'
Cuts at: 0 1037 1242
Size: 1037 205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 1242
Size: 526 716
Bpu10I CC'TnA_GC
Cuts at: 0 236 1242
Size: 236 1006
BsaI GGTCTCn'nnnn_
Cuts at: 0 771 1242
Size: 771 471
BsaAI yAC'GTr
Cuts at: 0 882 1242
Size: 882 360
BsaHI Gr'CG_yC
Cuts at: 0 142 862 1202 1242
Size: 142 720 340 40
Fragments arranged by size:
720 340 142 40
BsaWI w'CCGG_w
Cuts at: 0 1008 1150 1242
Size: 1008 142 92
Fragments arranged by size:
1008 142 92
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1192 1222 1242
Size: 1192 30 20
Fragments arranged by size:
1192 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 370 1051 1242
Size: 370 681 191
Fragments arranged by size:
681 370 191
BseYI C'CCAG_C
Cuts at: 0 78 303 498 868 1242
Size: 78 225 195 370 374
Fragments arranged by size:
374 370 225 195 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 397 451 1084 1242
Size: 262 135 54 633 158
Fragments arranged by size:
633 262 158 135 54
BsiEI CG_ry'CG
Cuts at: 0 167 211 415 494 516 743 1242
Size: 167 44 204 79 22 227 499
Fragments arranged by size:
499 227 204 167 79 44 22
BsiHKAI G_wGCw'C
Cuts at: 0 162 1061 1242
Size: 162 899 181
Fragments arranged by size:
899 181 162
Bsp1286I G_dGCh'C
Cuts at: 0 85 112 162 505 833 904 988 1061
Size: 85 27 50 343 328 71 84 73
Cuts at: 1061 1242
Size: 181
Fragments arranged by size:
343 328 181 85 84 73 71 50
27
BspEI T'CCGG_A
Cuts at: 0 1008 1242
Size: 1008 234
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 284 420 753 1242
Size: 284 136 333 489
Fragments arranged by size:
489 333 284 136
BsrBI CCG'CTC
Cuts at: 0 366 639 1242
Size: 366 273 603
Fragments arranged by size:
603 366 273
BsrFI r'CCGG_y
Cuts at: 0 144 162 410 568 742 799 1242
Size: 144 18 248 158 174 57 443
Fragments arranged by size:
443 248 174 158 144 57 18
BssHII G'CGCG_C
Cuts at: 0 53 629 1242
Size: 53 576 613
Fragments arranged by size:
613 576 53
BstAPI GCAn_nnn'nTGC
Cuts at: 0 384 1242
Size: 384 858
BtgI C'CryG_G
Cuts at: 0 62 173 415 524 833 1173 1211 1242
Size: 62 111 242 109 309 340 38 31
Fragments arranged by size:
340 309 242 111 109 62 38 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 910 1242
Size: 910 332
BtsI GCAGTG_nn'
Cuts at: 0 1015 1242
Size: 1015 227
DraIII CAC_nnn'GTG
Cuts at: 0 1107 1242
Size: 1107 135
EaeI y'GGCC_r
Cuts at: 0 71 164 208 412 491 513 521 600
Size: 71 93 44 204 79 22 8 79
Cuts at: 600 740 1242
Size: 140 502
Fragments arranged by size:
502 204 140 93 79 79 71 44
22 8
EagI C'GGCC_G
Cuts at: 0 164 208 412 491 513 740 1242
Size: 164 44 204 79 22 227 502
Fragments arranged by size:
502 227 204 164 79 44 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 349 1242
Size: 349 893
EcoICRI GAG'CTC
Cuts at: 0 1059 1242
Size: 1059 183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 526 1242
Size: 463 63 716
Fragments arranged by size:
716 463 63
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1066 1242
Size: 1066 176
EcoO109I rG'GnC_Cy
Cuts at: 0 82 502 838 985 1242
Size: 82 420 336 147 257
Fragments arranged by size:
420 336 257 147 82
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 363 1242
Size: 363 879
HaeII r_GCGC'y
Cuts at: 0 145 325 865 875 1242
Size: 145 180 540 10 367
Fragments arranged by size:
540 367 180 145 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1192 1224 1242
Size: 1192 32 18
Fragments arranged by size:
1192 32 18
KasI G'GCGC_C
Cuts at: 0 141 861 1242
Size: 141 720 381
Fragments arranged by size:
720 381 141
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1223 1242
Size: 1223 19
MscI TGG'CCA
Cuts at: 0 523 1242
Size: 523 719
MslI CAynn'nnrTG
Cuts at: 0 127 1242
Size: 127 1115
MspA1I CmG'CkG
Cuts at: 0 64 155 175 417 579 678 835 1213
Size: 64 91 20 242 162 99 157 378
Cuts at: 1213 1242
Size: 29
Fragments arranged by size:
378 242 162 157 99 91 64 29
20
NaeI GCC'GGC
Cuts at: 0 146 570 744 1242
Size: 146 424 174 498
Fragments arranged by size:
498 424 174 146
NarI GG'CG_CC
Cuts at: 0 142 862 1242
Size: 142 720 380
Fragments arranged by size:
720 380 142
NcoI C'CATG_G
Cuts at: 0 524 1242
Size: 524 718
NgoMIV G'CCGG_C
Cuts at: 0 144 568 742 1242
Size: 144 424 174 500
Fragments arranged by size:
500 424 174 144
NotI GC'GGCC_GC
Cuts at: 0 491 513 1242
Size: 491 22 729
Fragments arranged by size:
729 491 22
NspI r_CATG'y
Cuts at: 0 387 1242
Size: 387 855
PasI CC'CwG_GG
Cuts at: 0 92 1242
Size: 92 1150
PflMI CCAn_nnn'nTGG
Cuts at: 0 624 1242
Size: 624 618
PmlI CAC'GTG
Cuts at: 0 882 1242
Size: 882 360
PpuMI rG'GwC_Cy
Cuts at: 0 838 1242
Size: 838 404
PshAI GACnn'nnGTC
Cuts at: 0 996 1043 1242
Size: 996 47 199
Fragments arranged by size:
996 199 47
PspOMI G'GGCC_C
Cuts at: 0 81 501 984 1242
Size: 81 420 483 258
Fragments arranged by size:
483 420 258 81
SacI G_AGCT'C
Cuts at: 0 1061 1242
Size: 1061 181
SacII CC_GC'GG
Cuts at: 0 65 176 418 836 1214 1242
Size: 65 111 242 418 378 28
Fragments arranged by size:
418 378 242 111 65 28
SanDI GG'GwC_CC
Cuts at: 0 838 1242
Size: 838 404
SfcI C'TryA_G
Cuts at: 0 750 1242
Size: 750 492
SfoI GGC'GCC
Cuts at: 0 143 863 1242
Size: 143 720 379
Fragments arranged by size:
720 379 143
SgrAI Cr'CCGG_yG
Cuts at: 0 144 1242
Size: 144 1098
SmaI CCC'GGG
Cuts at: 0 726 909 983 1242
Size: 726 183 74 259
Fragments arranged by size:
726 259 183 74
StyI C'CwwG_G
Cuts at: 0 185 524 1242
Size: 185 339 718
Fragments arranged by size:
718 339 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 882 1242
Size: 882 360
TatI w'GTAC_w
Cuts at: 0 884 1242
Size: 884 358
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 531 1188 1242
Size: 531 657 54
Fragments arranged by size:
657 531 54
XmaI C'CCGG_G
Cuts at: 0 724 907 981 1242
Size: 724 183 74 261
Fragments arranged by size:
724 261 183 74
XmnI GAAnn'nnTTC
Cuts at: 0 220 1242
Size: 220 1022
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApaI ApoI AscI
AvaI BaeI BanI BanII BbeI BciVI BglI BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspMI BsrBI BsrFI BssHII BstAPI BtgI BtgZI
BtsI DraIII EaeI EagI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI
PflMI PmlI PpuMI PshAI PspOMI SacI SacII SanDI
SfcI SfoI SgrAI SmaI StyI TaqII TatI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaLI AseI AsiSI AvrII BamHI BbsI
BbvCI BcgI BclI BfrBI BglII BmtI BplI BpuEI
BsaBI BsiWI BsmI BsmBI BspHI BsrDI BsrGI BssSI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I ClaI CspCI
DraI DrdI EarI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI NdeI NheI
NruI NsiI PacI PciI PfoI PmeI PpiI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SalI SapI
SbfI ScaI SexAI SfiI SgfI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XhoI ZraI