Restriction map for
pF1KB9729
(Linear) (Six-base) MAPSORT of: KIBB9729.seq Check: 9949 from: 1 to: 1338
KIBB9729.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 0 162 1338
Size: 162 1176
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 827 1338
Size: 827 511
AfeI AGC'GCT
Cuts at: 0 1083 1338
Size: 1083 255
AhdI GACnn_n'nnGTC
Cuts at: 0 1177 1338
Size: 1177 161
AleI CACnn'nnGTG
Cuts at: 0 574 1338
Size: 574 764
AlwNI CAG_nnn'CTG
Cuts at: 0 48 873 1338
Size: 48 825 465
Fragments arranged by size:
825 465 48
ApaLI G'TGCA_C
Cuts at: 0 1094 1338
Size: 1094 244
ApoI r'AATT_y
Cuts at: 0 316 1338
Size: 316 1022
AvaI C'yCGr_G
Cuts at: 0 461 743 896 1338
Size: 461 282 153 442
Fragments arranged by size:
461 442 282 153
AvrII C'CTAG_G
Cuts at: 0 1009 1111 1338
Size: 1009 102 227
Fragments arranged by size:
1009 227 102
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 10 43 1338
Size: 10 33 1295
Fragments arranged by size:
1295 33 10
BanI G'GyrC_C
Cuts at: 0 193 436 605 636 1338
Size: 193 243 169 31 702
Fragments arranged by size:
702 243 193 169 31
BanII G_rGCy'C
Cuts at: 0 281 752 1338
Size: 281 471 586
Fragments arranged by size:
586 471 281
BbeI G_GCGC'C
Cuts at: 0 197 440 609 640 1338
Size: 197 243 169 31 698
Fragments arranged by size:
698 243 197 169 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 776 815 1199 1338
Size: 776 39 384 139
Fragments arranged by size:
776 384 139 39
Bme1580I G_kGCm'C
Cuts at: 0 300 1098 1282 1338
Size: 300 798 184 56
Fragments arranged by size:
798 300 184 56
BmrI ACTGGGnnnn_n'
Cuts at: 0 36 1338
Size: 36 1302
BmtI G_CTAG'C
Cuts at: 0 734 1338
Size: 734 604
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 300 705 1338
Size: 300 405 633
Fragments arranged by size:
633 405 300
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 1338
Size: 796 542
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 595 1338
Size: 466 129 743
Fragments arranged by size:
743 466 129
BsaI GGTCTCn'nnnn_
Cuts at: 0 460 1061 1338
Size: 460 601 277
Fragments arranged by size:
601 460 277
BsaHI Gr'CG_yC
Cuts at: 0 52 194 437 606 637 1338
Size: 52 142 243 169 31 701
Fragments arranged by size:
701 243 169 142 52 31
BsaWI w'CCGG_w
Cuts at: 0 183 964 1338
Size: 183 781 374
Fragments arranged by size:
781 374 183
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 673 692 697 799 802 805 808 811
Size: 673 19 5 102 3 3 3 3
Cuts at: 811 829 1302 1338
Size: 18 473 36
Fragments arranged by size:
673 473 102 36 19 18 5 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 126 168 189 832 1101 1338
Size: 126 42 21 643 269 237
Fragments arranged by size:
643 269 237 126 42 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1324 1338
Size: 1324 14
BsiEI CG_ry'CG
Cuts at: 0 184 230 444 1338
Size: 184 46 214 894
Fragments arranged by size:
894 214 184 46
BsiHKAI G_wGCw'C
Cuts at: 0 219 503 1098 1338
Size: 219 284 595 240
Fragments arranged by size:
595 284 240 219
Bsp1286I G_dGCh'C
Cuts at: 0 219 281 300 503 752 1098 1282 1338
Size: 219 62 19 203 249 346 184 56
Fragments arranged by size:
346 249 219 203 184 62 56 19
BspEI T'CCGG_A
Cuts at: 0 964 1338
Size: 964 374
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 269 1324 1338
Size: 269 1055 14
Fragments arranged by size:
1055 269 14
BsrBI CCG'CTC
Cuts at: 0 136 384 1338
Size: 136 248 954
Fragments arranged by size:
954 248 136
BsrFI r'CCGG_y
Cuts at: 0 439 602 1338
Size: 439 163 736
Fragments arranged by size:
736 439 163
BssHII G'CGCG_C
Cuts at: 0 863 1338
Size: 863 475
BstAPI GCAn_nnn'nTGC
Cuts at: 0 74 223 873 923 1338
Size: 74 149 650 50 415
Fragments arranged by size:
650 415 149 74 50
BtgI C'CryG_G
Cuts at: 0 30 1282 1338
Size: 30 1252 56
Fragments arranged by size:
1252 56 30
BtsI GCAGTG_nn'
Cuts at: 0 180 428 871 1312 1338
Size: 180 248 443 441 26
Fragments arranged by size:
443 441 248 180 26
EaeI y'GGCC_r
Cuts at: 0 227 545 824 1338
Size: 227 318 279 514
Fragments arranged by size:
514 318 279 227
EagI C'GGCC_G
Cuts at: 0 227 1338
Size: 227 1111
EarI CTCTTCn'nnn_
Cuts at: 0 776 1338
Size: 776 562
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1009 1338
Size: 1009 329
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 827 1338
Size: 796 31 511
Fragments arranged by size:
796 511 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 571 1338
Size: 571 767
FspI TGC'GCA
Cuts at: 0 147 1338
Size: 147 1191
HaeII r_GCGC'y
Cuts at: 0 38 133 197 440 609 640 1085 1338
Size: 38 95 64 243 169 31 445 253
Fragments arranged by size:
445 253 243 169 95 64 38 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 973 1005 1163 1195 1338
Size: 97 32 844 32 158 32 143
Fragments arranged by size:
844 158 143 97 32 32 32
HincII GTy'rAC
Cuts at: 0 163 1016 1338
Size: 163 853 322
Fragments arranged by size:
853 322 163
KasI G'GCGC_C
Cuts at: 0 193 436 605 636 1338
Size: 193 243 169 31 702
Fragments arranged by size:
702 243 193 169 31
MscI TGG'CCA
Cuts at: 0 547 826 1338
Size: 547 279 512
Fragments arranged by size:
547 512 279
MslI CAynn'nnrTG
Cuts at: 0 559 574 1338
Size: 559 15 764
Fragments arranged by size:
764 559 15
MspA1I CmG'CkG
Cuts at: 0 32 232 238 832 1263 1284 1338
Size: 32 200 6 594 431 21 54
Fragments arranged by size:
594 431 200 54 32 21 6
NaeI GCC'GGC
Cuts at: 0 604 1338
Size: 604 734
NarI GG'CG_CC
Cuts at: 0 194 437 606 637 1338
Size: 194 243 169 31 701
Fragments arranged by size:
701 243 194 169 31
NgoMIV G'CCGG_C
Cuts at: 0 602 1338
Size: 602 736
NheI G'CTAG_C
Cuts at: 0 730 1338
Size: 730 608
NotI GC'GGCC_GC
Cuts at: 0 227 1338
Size: 227 1111
NspI r_CATG'y
Cuts at: 0 77 1338
Size: 77 1261
PflMI CCAn_nnn'nTGG
Cuts at: 0 16 48 1338
Size: 16 32 1290
Fragments arranged by size:
1290 32 16
PfoI T'CCnGG_A
Cuts at: 0 1143 1338
Size: 1143 195
PstI C_TGCA'G
Cuts at: 0 237 333 894 1253 1338
Size: 237 96 561 359 85
Fragments arranged by size:
561 359 237 96 85
PvuII CAG'CTG
Cuts at: 0 238 832 1338
Size: 238 594 506
Fragments arranged by size:
594 506 238
SacII CC_GC'GG
Cuts at: 0 33 1285 1338
Size: 33 1252 53
Fragments arranged by size:
1252 53 33
SalI G'TCGA_C
Cuts at: 0 161 1338
Size: 161 1177
SbfI CC_TGCA'GG
Cuts at: 0 1253 1338
Size: 1253 85
SexAI A'CCwGG_T
Cuts at: 0 583 1338
Size: 583 755
SfcI C'TryA_G
Cuts at: 0 233 267 329 890 1249 1298 1338
Size: 233 34 62 561 359 49 40
Fragments arranged by size:
561 359 233 62 49 40 34
SfoI GGC'GCC
Cuts at: 0 195 438 607 638 1338
Size: 195 243 169 31 700
Fragments arranged by size:
700 243 195 169 31
SgrAI Cr'CCGG_yG
Cuts at: 0 602 1338
Size: 602 736
SmaI CCC'GGG
Cuts at: 0 463 745 1338
Size: 463 282 593
Fragments arranged by size:
593 463 282
SmlI C'TyrA_G
Cuts at: 0 481 610 896 1338
Size: 481 129 286 442
Fragments arranged by size:
481 442 286 129
StuI AGG'CCT
Cuts at: 0 487 1338
Size: 487 851
StyI C'CwwG_G
Cuts at: 0 200 1009 1111 1338
Size: 200 809 102 227
Fragments arranged by size:
809 227 200 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 586 1338
Size: 586 752
TatI w'GTAC_w
Cuts at: 0 475 1338
Size: 475 863
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 250 1318 1338
Size: 250 1068 20
Fragments arranged by size:
1068 250 20
XhoI C'TCGA_G
Cuts at: 0 896 1338
Size: 896 442
XmaI C'CCGG_G
Cuts at: 0 461 743 1338
Size: 461 282 595
Fragments arranged by size:
595 461 282
Enzymes that do cut:
AccI AcuI AfeI AhdI AleI AlwNI ApaLI ApoI
AvaI AvrII BaeI BanI BanII BbeI BbsI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI
BsrBI BsrFI BssHII BstAPI BtgI BtsI EaeI EagI
EarI EciI Eco57MI EcoNI FspI HaeII Hin4I HincII
KasI MscI MslI MspA1I NaeI NarI NgoMIV NheI
NotI NspI PflMI PfoI PstI PvuII SacII SalI
SbfI SexAI SfcI SfoI SgrAI SmaI SmlI StuI
StyI TaqII TatI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AlfI
AloI ApaI AscI AseI AsiSI BamHI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI Bpu10I
BsaAI BsaBI BsaXI BsiWI BsmI BsmBI BspHI BsrDI
BsrGI BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI EcoO109I
EcoRI EcoRV FalI FseI FspAI HindIII HpaI KpnI
MfeI MluI MmeI NcoI NdeI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SanDI SapI
ScaI SfiI SgfI SnaBI SpeI SphI SrfI SspI
SwaI TstI Tth111I XbaI XmnI ZraI