Restriction map for
pF1KB9730
(Linear) (Six-base) MAPSORT of: KIBB9730.seq Check: 5597 from: 1 to: 1395
KIBB9730.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 140 1395
Size: 140 1255
AfeI AGC'GCT
Cuts at: 0 919 1395
Size: 919 476
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 148 1395
Size: 148 1247
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1057 1089 1395
Size: 1057 32 306
Fragments arranged by size:
1057 306 32
AlwNI CAG_nnn'CTG
Cuts at: 0 333 420 1263 1395
Size: 333 87 843 132
Fragments arranged by size:
843 333 132 87
ApaI G_GGCC'C
Cuts at: 0 222 1395
Size: 222 1173
AvaI C'yCGr_G
Cuts at: 0 606 803 1395
Size: 606 197 592
Fragments arranged by size:
606 592 197
BanI G'GyrC_C
Cuts at: 0 60 723 1395
Size: 60 663 672
Fragments arranged by size:
672 663 60
BanII G_rGCy'C
Cuts at: 0 222 590 812 914 1395
Size: 222 368 222 102 481
Fragments arranged by size:
481 368 222 222 102
BbsI GAAGACnn'nnnn_
Cuts at: 0 1164 1395
Size: 1164 231
BglI GCCn_nnn'nGGC
Cuts at: 0 239 425 1395
Size: 239 186 970
Fragments arranged by size:
970 239 186
BlpI GC'TnA_GC
Cuts at: 0 1098 1276 1395
Size: 1098 178 119
Fragments arranged by size:
1098 178 119
Bme1580I G_kGCm'C
Cuts at: 0 65 222 728 799 1395
Size: 65 157 506 71 596
Fragments arranged by size:
596 506 157 71 65
BmgBI CAC'GTC
Cuts at: 0 513 1395
Size: 513 882
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1362 1395
Size: 1362 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 531 1029 1395
Size: 531 498 366
Fragments arranged by size:
531 498 366
Bpu10I CC'TnA_GC
Cuts at: 0 224 1395
Size: 224 1171
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 1395
Size: 898 497
BsaI GGTCTCn'nnnn_
Cuts at: 0 1027 1395
Size: 1027 368
BsaHI Gr'CG_yC
Cuts at: 0 166 1395
Size: 166 1229
BsaWI w'CCGG_w
Cuts at: 0 553 1040 1395
Size: 553 487 355
Fragments arranged by size:
553 487 355
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 136 166 1057 1087 1395
Size: 136 30 891 30 308
Fragments arranged by size:
891 308 136 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1329 1395
Size: 1329 66
BseYI C'CCAG_C
Cuts at: 0 81 578 1256 1263 1395
Size: 81 497 678 7 132
Fragments arranged by size:
678 497 132 81 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 1395
Size: 22 1373
BsiEI CG_ry'CG
Cuts at: 0 29 681 716 1029 1395
Size: 29 652 35 313 366
Fragments arranged by size:
652 366 313 35 29
BsiHKAI G_wGCw'C
Cuts at: 0 230 914 959 1282 1395
Size: 230 684 45 323 113
Fragments arranged by size:
684 323 230 113 45
Bsp1286I G_dGCh'C
Cuts at: 0 65 222 230 590 728 799 812 914
Size: 65 157 8 360 138 71 13 102
Cuts at: 914 959 1282 1395
Size: 45 323 113
Fragments arranged by size:
360 323 157 138 113 102 71 65
45 13 8
BspEI T'CCGG_A
Cuts at: 0 1040 1395
Size: 1040 355
BspHI T'CATG_A
Cuts at: 0 1298 1395
Size: 1298 97
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 86 140 825 995 1395
Size: 86 54 685 170 400
Fragments arranged by size:
685 400 170 86 54
BsrBI CCG'CTC
Cuts at: 0 437 965 1395
Size: 437 528 430
Fragments arranged by size:
528 437 430
BsrDI GCAATG_nn'
Cuts at: 0 849 1395
Size: 849 546
BsrFI r'CCGG_y
Cuts at: 0 57 238 517 715 826 1395
Size: 57 181 279 198 111 569
Fragments arranged by size:
569 279 198 181 111 57
BssHII G'CGCG_C
Cuts at: 0 100 830 1395
Size: 100 730 565
Fragments arranged by size:
730 565 100
BstAPI GCAn_nnn'nTGC
Cuts at: 0 333 954 1395
Size: 333 621 441
Fragments arranged by size:
621 441 333
BstBI TT'CG_AA
Cuts at: 0 653 1395
Size: 653 742
BstEII G'GTnAC_C
Cuts at: 0 822 1395
Size: 822 573
BstXI CCAn_nnnn'nTGG
Cuts at: 0 162 264 888 1395
Size: 162 102 624 507
Fragments arranged by size:
624 507 162 102
BtgI C'CryG_G
Cuts at: 0 17 110 120 155 263 684 780 1395
Size: 17 93 10 35 108 421 96 615
Fragments arranged by size:
615 421 108 96 93 35 17 10
DraIII CAC_nnn'GTG
Cuts at: 0 45 1395
Size: 45 1350
EaeI y'GGCC_r
Cuts at: 0 236 515 668 678 713 1026 1395
Size: 236 279 153 10 35 313 369
Fragments arranged by size:
369 313 279 236 153 35 10
EagI C'GGCC_G
Cuts at: 0 678 713 1026 1395
Size: 678 35 313 369
Fragments arranged by size:
678 369 313 35
EarI CTCTTCn'nnn_
Cuts at: 0 863 1395
Size: 863 532
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 209 269 1395
Size: 209 60 1126
Fragments arranged by size:
1126 209 60
EcoICRI GAG'CTC
Cuts at: 0 912 1395
Size: 912 483
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 531 1029 1395
Size: 531 498 366
Fragments arranged by size:
531 498 366
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1041 1395
Size: 1041 354
EcoO109I rG'GnC_Cy
Cuts at: 0 219 974 1395
Size: 219 755 421
Fragments arranged by size:
755 421 219
FseI GG_CCGG'CC
Cuts at: 0 242 1395
Size: 242 1153
FspI TGC'GCA
Cuts at: 0 994 1395
Size: 994 401
HaeII r_GCGC'y
Cuts at: 0 420 921 1395
Size: 420 501 474
Fragments arranged by size:
501 474 420
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1250 1395
Size: 1250 145
MslI CAynn'nnrTG
Cuts at: 0 160 262 451 1395
Size: 160 102 189 944
Fragments arranged by size:
944 189 160 102
MspA1I CmG'CkG
Cuts at: 0 12 122 188 333 1263 1395
Size: 12 110 66 145 930 132
Fragments arranged by size:
930 145 132 110 66 12
NaeI GCC'GGC
Cuts at: 0 240 519 717 1395
Size: 240 279 198 678
Fragments arranged by size:
678 279 240 198
NcoI C'CATG_G
Cuts at: 0 17 155 263 1395
Size: 17 138 108 1132
Fragments arranged by size:
1132 138 108 17
NgoMIV G'CCGG_C
Cuts at: 0 238 517 715 1395
Size: 238 279 198 680
Fragments arranged by size:
680 279 238 198
NspI r_CATG'y
Cuts at: 0 297 1086 1109 1204 1395
Size: 297 789 23 95 191
Fragments arranged by size:
789 297 191 95 23
PasI CC'CwG_GG
Cuts at: 0 774 969 1395
Size: 774 195 426
Fragments arranged by size:
774 426 195
PflMI CCAn_nnn'nTGG
Cuts at: 0 420 1007 1263 1395
Size: 420 587 256 132
Fragments arranged by size:
587 420 256 132
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1057 1089 1395
Size: 1057 32 306
Fragments arranged by size:
1057 306 32
PpuMI rG'GwC_Cy
Cuts at: 0 974 1395
Size: 974 421
PspOMI G'GGCC_C
Cuts at: 0 218 1395
Size: 218 1177
PstI C_TGCA'G
Cuts at: 0 329 1064 1395
Size: 329 735 331
Fragments arranged by size:
735 331 329
PvuI CG_AT'CG
Cuts at: 0 29 1395
Size: 29 1366
PvuII CAG'CTG
Cuts at: 0 12 1263 1395
Size: 12 1251 132
Fragments arranged by size:
1251 132 12
SacI G_AGCT'C
Cuts at: 0 914 1395
Size: 914 481
SacII CC_GC'GG
Cuts at: 0 123 1395
Size: 123 1272
SanDI GG'GwC_CC
Cuts at: 0 974 1395
Size: 974 421
SfcI C'TryA_G
Cuts at: 0 325 921 1060 1395
Size: 325 596 139 335
Fragments arranged by size:
596 335 325 139
SmaI CCC'GGG
Cuts at: 0 608 805 1395
Size: 608 197 590
Fragments arranged by size:
608 590 197
SmlI C'TyrA_G
Cuts at: 0 913 1395
Size: 913 482
SphI G_CATG'C
Cuts at: 0 297 1109 1395
Size: 297 812 286
Fragments arranged by size:
812 297 286
SspI AAT'ATT
Cuts at: 0 1208 1395
Size: 1208 187
StyI C'CwwG_G
Cuts at: 0 17 155 263 1395
Size: 17 138 108 1132
Fragments arranged by size:
1132 138 108 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1167 1395
Size: 1167 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 136 168 1395
Size: 136 32 1227
Fragments arranged by size:
1227 136 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 261 555 1395
Size: 261 294 840
Fragments arranged by size:
840 294 261
XmaI C'CCGG_G
Cuts at: 0 606 803 1395
Size: 606 197 592
Fragments arranged by size:
606 592 197
XmnI GAAnn'nnTTC
Cuts at: 0 852 1395
Size: 852 543
Enzymes that do cut:
AarI AfeI AlfI AloI AlwNI ApaI AvaI BanI
BanII BbsI BglI BlpI Bme1580I BmgBI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BstAPI BstBI BstEII BstXI BtgI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FseI FspI HaeII MmeI MslI MspA1I NaeI
NcoI NgoMIV NspI PasI PflMI PpiI PpuMI PspOMI
PstI PvuI PvuII SacI SacII SanDI SfcI SmaI
SmlI SphI SspI StyI TaqII TstI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglII BmrI BmtI BsaAI BsaBI BsiWI BsmI BsmBI
BsrGI BssSI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DrdI EcoRI EcoRV FalI FspAI Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
NarI NdeI NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PshAI PsiI PspXI PsrI RsrII
SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SrfI StuI SwaI TatI Tth111I
XbaI XhoI ZraI