Restriction map for
pF1KB9731
(Linear) (Six-base) MAPSORT of: KIBB9731.seq Check: 3127 from: 1 to: 1422
KIBB9731.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 84 689 936 1422
Size: 84 605 247 486
Fragments arranged by size:
605 486 247 84
AccI GT'mk_AC
Cuts at: 0 23 1422
Size: 23 1399
AflIII A'CryG_T
Cuts at: 0 116 275 1422
Size: 116 159 1147
Fragments arranged by size:
1147 159 116
AleI CACnn'nnGTG
Cuts at: 0 215 1422
Size: 215 1207
AlwNI CAG_nnn'CTG
Cuts at: 0 585 834 1327 1422
Size: 585 249 493 95
Fragments arranged by size:
585 493 249 95
ApoI r'AATT_y
Cuts at: 0 79 1422
Size: 79 1343
AvaI C'yCGr_G
Cuts at: 0 222 1422
Size: 222 1200
AvrII C'CTAG_G
Cuts at: 0 732 1422
Size: 732 690
BanI G'GyrC_C
Cuts at: 0 133 217 1422
Size: 133 84 1205
Fragments arranged by size:
1205 133 84
BanII G_rGCy'C
Cuts at: 0 632 666 1334 1422
Size: 632 34 668 88
Fragments arranged by size:
668 632 88 34
BbvCI CC'TCA_GC
Cuts at: 0 1234 1422
Size: 1234 188
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 28 62 1422
Size: 28 34 1360
Fragments arranged by size:
1360 34 28
BlpI GC'TnA_GC
Cuts at: 0 904 1422
Size: 904 518
BmgBI CAC'GTC
Cuts at: 0 119 723 1422
Size: 119 604 699
Fragments arranged by size:
699 604 119
Bpu10I CC'TnA_GC
Cuts at: 0 1234 1320 1422
Size: 1234 86 102
Fragments arranged by size:
1234 102 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 1422
Size: 334 1088
BsaI GGTCTCn'nnnn_
Cuts at: 0 1285 1422
Size: 1285 137
BsaWI w'CCGG_w
Cuts at: 0 251 404 1422
Size: 251 153 1018
Fragments arranged by size:
1018 251 153
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 265 295 466 496 1422
Size: 265 30 171 30 926
Fragments arranged by size:
926 265 171 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 270 1422
Size: 270 1152
BseYI C'CCAG_C
Cuts at: 0 60 615 1122 1135 1391 1422
Size: 60 555 507 13 256 31
Fragments arranged by size:
555 507 256 60 31 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 112 964 1150 1422
Size: 35 77 852 186 272
Fragments arranged by size:
852 272 186 77 35
BsiEI CG_ry'CG
Cuts at: 0 975 1422
Size: 975 447
BsiHKAI G_wGCw'C
Cuts at: 0 632 680 1422
Size: 632 48 742
Fragments arranged by size:
742 632 48
BsmBI CGTCTCn'nnnn_
Cuts at: 0 510 1422
Size: 510 912
Bsp1286I G_dGCh'C
Cuts at: 0 632 666 680 1334 1422
Size: 632 34 14 654 88
Fragments arranged by size:
654 632 88 34 14
BspEI T'CCGG_A
Cuts at: 0 251 1422
Size: 251 1171
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 84 689 936 1422
Size: 84 605 247 486
Fragments arranged by size:
605 486 247 84
BsrBI CCG'CTC
Cuts at: 0 399 1422
Size: 399 1023
BsrDI GCAATG_nn'
Cuts at: 0 117 744 1422
Size: 117 627 678
Fragments arranged by size:
678 627 117
BsrFI r'CCGG_y
Cuts at: 0 296 1422
Size: 296 1126
BstAPI GCAn_nnn'nTGC
Cuts at: 0 684 1116 1150 1422
Size: 684 432 34 272
Fragments arranged by size:
684 432 272 34
BstXI CCAn_nnnn'nTGG
Cuts at: 0 159 1422
Size: 159 1263
BstYI r'GATC_y
Cuts at: 0 703 1061 1422
Size: 703 358 361
Fragments arranged by size:
703 361 358
BtgI C'CryG_G
Cuts at: 0 200 707 1422
Size: 200 507 715
Fragments arranged by size:
715 507 200
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 534 1154 1422
Size: 534 620 268
Fragments arranged by size:
620 534 268
BtsI GCAGTG_nn'
Cuts at: 0 307 759 1422
Size: 307 452 663
Fragments arranged by size:
663 452 307
ClaI AT'CG_AT
Cuts at: 0 842 1422
Size: 842 580
DrdI GACnn_nn'nnGTC
Cuts at: 0 748 1422
Size: 748 674
EaeI y'GGCC_r
Cuts at: 0 35 993 1088 1422
Size: 35 958 95 334
Fragments arranged by size:
958 334 95 35
EarI CTCTTCn'nnn_
Cuts at: 0 1084 1422
Size: 1084 338
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 487 790 1422
Size: 487 303 632
Fragments arranged by size:
632 487 303
EcoICRI GAG'CTC
Cuts at: 0 630 1422
Size: 630 792
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1108 1180 1422
Size: 1108 72 242
Fragments arranged by size:
1108 242 72
EcoO109I rG'GnC_Cy
Cuts at: 0 477 1130 1422
Size: 477 653 292
Fragments arranged by size:
653 477 292
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 264 375 1422
Size: 264 111 1047
Fragments arranged by size:
1047 264 111
HaeII r_GCGC'y
Cuts at: 0 173 1422
Size: 173 1249
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 399 431 466 498 1422
Size: 399 32 35 32 924
Fragments arranged by size:
924 399 35 32 32
HincII GTy'rAC
Cuts at: 0 24 101 1422
Size: 24 77 1321
Fragments arranged by size:
1321 77 24
MscI TGG'CCA
Cuts at: 0 1090 1422
Size: 1090 332
MslI CAynn'nnrTG
Cuts at: 0 31 215 1422
Size: 31 184 1207
Fragments arranged by size:
1207 184 31
MspA1I CmG'CkG
Cuts at: 0 582 1422
Size: 582 840
NaeI GCC'GGC
Cuts at: 0 298 1422
Size: 298 1124
NcoI C'CATG_G
Cuts at: 0 707 1422
Size: 707 715
NdeI CA'TA_TG
Cuts at: 0 1190 1422
Size: 1190 232
NgoMIV G'CCGG_C
Cuts at: 0 296 1422
Size: 296 1126
NspI r_CATG'y
Cuts at: 0 279 1422
Size: 279 1143
PasI CC'CwG_GG
Cuts at: 0 1268 1422
Size: 1268 154
PciI A'CATG_T
Cuts at: 0 275 1422
Size: 275 1147
PfoI T'CCnGG_A
Cuts at: 0 508 827 1422
Size: 508 319 595
Fragments arranged by size:
595 508 319
PpuMI rG'GwC_Cy
Cuts at: 0 477 1130 1422
Size: 477 653 292
Fragments arranged by size:
653 477 292
PspXI vC'TCGA_Gb
Cuts at: 0 222 1422
Size: 222 1200
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 262 294 1422
Size: 262 32 1128
Fragments arranged by size:
1128 262 32
PstI C_TGCA'G
Cuts at: 0 289 346 470 839 1422
Size: 289 57 124 369 583
Fragments arranged by size:
583 369 289 124 57
PvuII CAG'CTG
Cuts at: 0 582 1422
Size: 582 840
SacI G_AGCT'C
Cuts at: 0 632 1422
Size: 632 790
SalI G'TCGA_C
Cuts at: 0 22 1422
Size: 22 1400
SanDI GG'GwC_CC
Cuts at: 0 1130 1422
Size: 1130 292
SapI GCTCTTCn'nnn_
Cuts at: 0 1084 1422
Size: 1084 338
SbfI CC_TGCA'GG
Cuts at: 0 470 1422
Size: 470 952
SexAI A'CCwGG_T
Cuts at: 0 644 1422
Size: 644 778
SfcI C'TryA_G
Cuts at: 0 42 285 342 466 835 1422
Size: 42 243 57 124 369 587
Fragments arranged by size:
587 369 243 124 57 42
SmlI C'TyrA_G
Cuts at: 0 222 349 1422
Size: 222 127 1073
Fragments arranged by size:
1073 222 127
StyI C'CwwG_G
Cuts at: 0 707 732 896 1422
Size: 707 25 164 526
Fragments arranged by size:
707 526 164 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 151 1422
Size: 151 1271
TatI w'GTAC_w
Cuts at: 0 278 608 1422
Size: 278 330 814
Fragments arranged by size:
814 330 278
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 265 297 1422
Size: 265 32 1125
Fragments arranged by size:
1125 265 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 705 1422
Size: 705 717
XhoI C'TCGA_G
Cuts at: 0 222 1422
Size: 222 1200
XmnI GAAnn'nnTTC
Cuts at: 0 358 1422
Size: 358 1064
Enzymes that do cut:
AarI AccI AflIII AleI AlwNI ApoI AvaI AvrII
BanI BanII BbvCI BcgI BlpI BmgBI Bpu10I BpuEI
BsaI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstAPI
BstXI BstYI BtgI BtgZI BtsI ClaI DrdI EaeI
EarI EciI EcoICRI EcoNI EcoO109I FalI HaeII Hin4I
HincII MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NspI PasI PciI PfoI PpuMI PspXI PsrI PstI
PvuII SacI SalI SanDI SapI SbfI SexAI SfcI
SmlI StyI TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI BaeI
BamHI BbeI BbsI BciVI BclI BfrBI BglI BglII
Bme1580I BmrI BmtI BplI BpmI BsaAI BsaBI BsaHI
BsiWI BsmI BspHI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I CspCI DraI DraIII EagI Eco57MI EcoRI
EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI MmeI NarI NheI NotI NruI NsiI
PacI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PvuI RsrII SacII ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XmaI ZraI