Restriction map for
pF1KB9734
(Linear) (Six-base) MAPSORT of: KIBB9734.seq Check: 866 from: 1 to: 1458
KIBB9734.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1208 1458
Size: 1208 250
AatII G_ACGT'C
Cuts at: 0 545 1458
Size: 545 913
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 1239 1458
Size: 74 1165 219
Fragments arranged by size:
1165 219 74
AfeI AGC'GCT
Cuts at: 0 78 581 1458
Size: 78 503 877
Fragments arranged by size:
877 503 78
AflIII A'CryG_T
Cuts at: 0 1322 1458
Size: 1322 136
AhdI GACnn_n'nnGTC
Cuts at: 0 729 1458
Size: 729 729
AleI CACnn'nnGTG
Cuts at: 0 1282 1458
Size: 1282 176
AlwNI CAG_nnn'CTG
Cuts at: 0 513 1458
Size: 513 945
ApaI G_GGCC'C
Cuts at: 0 645 1458
Size: 645 813
ApaLI G'TGCA_C
Cuts at: 0 1375 1458
Size: 1375 83
AvaI C'yCGr_G
Cuts at: 0 816 1458
Size: 816 642
BanI G'GyrC_C
Cuts at: 0 333 496 561 1079 1458
Size: 333 163 65 518 379
Fragments arranged by size:
518 379 333 163 65
BanII G_rGCy'C
Cuts at: 0 645 857 1190 1458
Size: 645 212 333 268
Fragments arranged by size:
645 333 268 212
BbeI G_GCGC'C
Cuts at: 0 565 1083 1458
Size: 565 518 375
Fragments arranged by size:
565 518 375
BbvCI CC'TCA_GC
Cuts at: 0 238 524 839 926 1458
Size: 238 286 315 87 532
Fragments arranged by size:
532 315 286 238 87
BglI GCCn_nnn'nGGC
Cuts at: 0 688 961 1088 1458
Size: 688 273 127 370
Fragments arranged by size:
688 370 273 127
Bme1580I G_kGCm'C
Cuts at: 0 275 501 645 1379 1458
Size: 275 226 144 734 79
Fragments arranged by size:
734 275 226 144 79
BmgBI CAC'GTC
Cuts at: 0 1323 1458
Size: 1323 135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 226 1458
Size: 152 74 1232
Fragments arranged by size:
1232 152 74
Bpu10I CC'TnA_GC
Cuts at: 0 10 238 524 839 926 1458
Size: 10 228 286 315 87 532
Fragments arranged by size:
532 315 286 228 87 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 1366 1396 1458
Size: 168 1198 30 62
Fragments arranged by size:
1198 168 62 30
BsaAI yAC'GTr
Cuts at: 0 1284 1458
Size: 1284 174
BsaHI Gr'CG_yC
Cuts at: 0 542 562 1080 1458
Size: 542 20 518 378
Fragments arranged by size:
542 518 378 20
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 213 243 418 448 1458
Size: 213 30 175 30 1010
Fragments arranged by size:
1010 213 175 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 1417 1458
Size: 28 1389 41
Fragments arranged by size:
1389 41 28
BseYI C'CCAG_C
Cuts at: 0 73 152 765 784 939 1434 1458
Size: 73 79 613 19 155 495 24
Fragments arranged by size:
613 495 155 79 73 24 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1001 1458
Size: 1001 457
BsiEI CG_ry'CG
Cuts at: 0 761 1123 1332 1458
Size: 761 362 209 126
Fragments arranged by size:
761 362 209 126
BsiHKAI G_wGCw'C
Cuts at: 0 1190 1379 1458
Size: 1190 189 79
Fragments arranged by size:
1190 189 79
Bsp1286I G_dGCh'C
Cuts at: 0 275 501 645 857 1190 1379 1458
Size: 275 226 144 212 333 189 79
Fragments arranged by size:
333 275 226 212 189 144 79
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1208 1458
Size: 1208 250
BsrBI CCG'CTC
Cuts at: 0 1407 1458
Size: 1407 51
BsrFI r'CCGG_y
Cuts at: 0 121 756 1297 1458
Size: 121 635 541 161
Fragments arranged by size:
635 541 161 121
BssHII G'CGCG_C
Cuts at: 0 1174 1342 1393 1395 1458
Size: 1174 168 51 2 63
Fragments arranged by size:
1174 168 63 51 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1106 1458
Size: 1106 352
BstYI r'GATC_y
Cuts at: 0 367 441 1458
Size: 367 74 1017
Fragments arranged by size:
1017 367 74
BtgI C'CryG_G
Cuts at: 0 113 959 1083 1117 1458
Size: 113 846 124 34 341
Fragments arranged by size:
846 341 124 113 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1364 1458
Size: 1364 94
BtsI GCAGTG_nn'
Cuts at: 0 275 514 1011 1458
Size: 275 239 497 447
Fragments arranged by size:
497 447 275 239
EaeI y'GGCC_r
Cuts at: 0 116 758 911 1120 1329 1458
Size: 116 642 153 209 209 129
Fragments arranged by size:
642 209 209 153 129 116
EagI C'GGCC_G
Cuts at: 0 758 1120 1329 1458
Size: 758 362 209 129
Fragments arranged by size:
758 362 209 129
EarI CTCTTCn'nnn_
Cuts at: 0 307 1458
Size: 307 1151
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 410 914 979 1003 1458
Size: 410 504 65 24 455
Fragments arranged by size:
504 455 410 65 24
EcoICRI GAG'CTC
Cuts at: 0 1188 1458
Size: 1188 270
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 152 226 1239 1458
Size: 74 78 74 1013 219
Fragments arranged by size:
1013 219 78 74 74
EcoNI CCTnn'n_nnAGG
Cuts at: 0 134 1458
Size: 134 1324
EcoO109I rG'GnC_Cy
Cuts at: 0 211 607 641 642 791 843 1416 1458
Size: 211 396 34 1 149 52 573 42
Fragments arranged by size:
573 396 211 149 52 42 34 1
FspI TGC'GCA
Cuts at: 0 1204 1288 1458
Size: 1204 84 170
Fragments arranged by size:
1204 170 84
FspAI rTGC'GCAy
Cuts at: 0 1288 1458
Size: 1288 170
HaeII r_GCGC'y
Cuts at: 0 80 452 565 583 956 1083 1458
Size: 80 372 113 18 373 127 375
Fragments arranged by size:
375 373 372 127 113 80 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 418 450 577 609 715 747 1458
Size: 418 32 127 32 106 32 711
Fragments arranged by size:
711 418 127 106 32 32 32
KasI G'GCGC_C
Cuts at: 0 561 1079 1458
Size: 561 518 379
Fragments arranged by size:
561 518 379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1205 1458
Size: 1205 253
MscI TGG'CCA
Cuts at: 0 913 1458
Size: 913 545
MslI CAynn'nnrTG
Cuts at: 0 1282 1458
Size: 1282 176
MspA1I CmG'CkG
Cuts at: 0 115 167 248 375 740 788 810 939
Size: 115 52 81 127 365 48 22 129
Cuts at: 939 961 1085 1119 1458
Size: 22 124 34 339
Fragments arranged by size:
365 339 129 127 124 115 81 52
48 34 22 22
NaeI GCC'GGC
Cuts at: 0 758 1458
Size: 758 700
NarI GG'CG_CC
Cuts at: 0 562 1080 1458
Size: 562 518 378
Fragments arranged by size:
562 518 378
NgoMIV G'CCGG_C
Cuts at: 0 756 1458
Size: 756 702
NotI GC'GGCC_GC
Cuts at: 0 1120 1329 1458
Size: 1120 209 129
Fragments arranged by size:
1120 209 129
PasI CC'CwG_GG
Cuts at: 0 620 646 850 1458
Size: 620 26 204 608
Fragments arranged by size:
620 608 204 26
PfoI T'CCnGG_A
Cuts at: 0 203 1458
Size: 203 1255
PmlI CAC'GTG
Cuts at: 0 1284 1458
Size: 1284 174
PpuMI rG'GwC_Cy
Cuts at: 0 607 1458
Size: 607 851
PspOMI G'GGCC_C
Cuts at: 0 641 1458
Size: 641 817
PvuII CAG'CTG
Cuts at: 0 167 740 1458
Size: 167 573 718
Fragments arranged by size:
718 573 167
SacI G_AGCT'C
Cuts at: 0 1190 1458
Size: 1190 268
SacII CC_GC'GG
Cuts at: 0 116 962 1086 1120 1458
Size: 116 846 124 34 338
Fragments arranged by size:
846 338 124 116 34
SanDI GG'GwC_CC
Cuts at: 0 607 1458
Size: 607 851
SfcI C'TryA_G
Cuts at: 0 243 1458
Size: 243 1215
SfoI GGC'GCC
Cuts at: 0 563 1081 1458
Size: 563 518 377
Fragments arranged by size:
563 518 377
SgrAI Cr'CCGG_yG
Cuts at: 0 1297 1458
Size: 1297 161
SmaI CCC'GGG
Cuts at: 0 818 1458
Size: 818 640
SmlI C'TyrA_G
Cuts at: 0 147 1381 1411 1458
Size: 147 1234 30 47
Fragments arranged by size:
1234 147 47 30
StuI AGG'CCT
Cuts at: 0 329 481 1135 1458
Size: 329 152 654 323
Fragments arranged by size:
654 329 323 152
StyI C'CwwG_G
Cuts at: 0 1061 1130 1458
Size: 1061 69 328
Fragments arranged by size:
1061 328 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1306 1458
Size: 1306 152
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 213 245 1458
Size: 213 32 1213
Fragments arranged by size:
1213 213 32
Tth111I GACn'n_nGTC
Cuts at: 0 589 1458
Size: 589 869
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 372 470 1458
Size: 372 98 988
Fragments arranged by size:
988 372 98
XmaI C'CCGG_G
Cuts at: 0 816 1458
Size: 816 642
ZraI GAC'GTC
Cuts at: 0 543 1458
Size: 543 915
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AhdI AleI AlwNI
ApaI ApaLI AvaI BanI BanII BbeI BbvCI BglI
Bme1580I BmgBI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BssHII BstAPI BstYI BtgI BtgZI BtsI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI
FspAI HaeII Hin4I KasI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NotI PasI PfoI PmlI PpuMI
PspOMI PvuII SacI SacII SanDI SfcI SfoI SgrAI
SmaI SmlI StuI StyI TaqII TstI Tth111I XcmI
XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AlfI AloI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BcgI
BciVI BclI BfrBI BglII BlpI BmrI BmtI BplI
BsaI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BssSI BstBI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FalI
FseI HincII HindIII HpaI KpnI MfeI MluI NcoI
NdeI NheI NruI NsiI NspI PacI PciI PflMI
PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SalI SapI SbfI ScaI SexAI SfiI SgfI
SnaBI SpeI SphI SrfI SspI SwaI TatI XbaI
XhoI XmnI