Restriction map for
pF1KB9735
(Linear) (Six-base) MAPSORT of: KIBB9735.seq Check: 4363 from: 1 to: 1449
KIBB9735.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AccI GT'mk_AC
Cuts at: 0 341 1449
Size: 341 1108
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 412 1358 1449
Size: 193 219 946 91
Fragments arranged by size:
946 219 193 91
AfeI AGC'GCT
Cuts at: 0 1009 1449
Size: 1009 440
AflIII A'CryG_T
Cuts at: 0 284 1449
Size: 284 1165
AleI CACnn'nnGTG
Cuts at: 0 853 1449
Size: 853 596
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 917 1449
Size: 917 532
AlwNI CAG_nnn'CTG
Cuts at: 0 1173 1201 1276 1449
Size: 1173 28 75 173
Fragments arranged by size:
1173 173 75 28
ApaI G_GGCC'C
Cuts at: 0 278 1449
Size: 278 1171
ApoI r'AATT_y
Cuts at: 0 106 229 1449
Size: 106 123 1220
Fragments arranged by size:
1220 123 106
AvaI C'yCGr_G
Cuts at: 0 33 1449
Size: 33 1416
BanI G'GyrC_C
Cuts at: 0 681 1449
Size: 681 768
BanII G_rGCy'C
Cuts at: 0 278 1449
Size: 278 1171
BciVI GTATCCnnnnn_n'
Cuts at: 0 709 1449
Size: 709 740
BfrBI ATG'CAT
Cuts at: 0 591 1449
Size: 591 858
BlpI GC'TnA_GC
Cuts at: 0 938 1293 1449
Size: 938 355 156
Fragments arranged by size:
938 355 156
Bme1580I G_kGCm'C
Cuts at: 0 278 686 1449
Size: 278 408 763
Fragments arranged by size:
763 408 278
BmrI ACTGGGnnnn_n'
Cuts at: 0 965 1152 1189 1428 1449
Size: 965 187 37 239 21
Fragments arranged by size:
965 239 187 37 21
BmtI G_CTAG'C
Cuts at: 0 215 1449
Size: 215 1234
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 360 1449
Size: 360 1089
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 1036 1253 1449
Size: 617 419 217 196
Fragments arranged by size:
617 419 217 196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1205 1449
Size: 1205 244
BsaI GGTCTCn'nnnn_
Cuts at: 0 566 933 1449
Size: 566 367 516
Fragments arranged by size:
566 516 367
BsaBI GATnn'nnATC
Cuts at: 0 886 986 1449
Size: 886 100 463
Fragments arranged by size:
886 463 100
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 141 171 519 549 787 817 1251 1281
Size: 141 30 348 30 238 30 434 30
Cuts at: 1281 1449
Size: 168
Fragments arranged by size:
434 348 238 168 141 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 199 795 1449
Size: 199 596 654
Fragments arranged by size:
654 596 199
BseYI C'CCAG_C
Cuts at: 0 114 271 917 1449
Size: 114 157 646 532
Fragments arranged by size:
646 532 157 114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1187 1449
Size: 1187 262
Bsp1286I G_dGCh'C
Cuts at: 0 278 686 1449
Size: 278 408 763
Fragments arranged by size:
763 408 278
BspHI T'CATG_A
Cuts at: 0 1418 1449
Size: 1418 31
BsrDI GCAATG_nn'
Cuts at: 0 33 137 1152 1317 1449
Size: 33 104 1015 165 132
Fragments arranged by size:
1015 165 132 104 33
BstEII G'GTnAC_C
Cuts at: 0 405 1449
Size: 405 1044
BstXI CCAn_nnnn'nTGG
Cuts at: 0 852 1449
Size: 852 597
BstYI r'GATC_y
Cuts at: 0 987 1449
Size: 987 462
BtgI C'CryG_G
Cuts at: 0 677 851 1449
Size: 677 174 598
Fragments arranged by size:
677 598 174
BtsI GCAGTG_nn'
Cuts at: 0 1199 1449
Size: 1199 250
DrdI GACnn_nn'nnGTC
Cuts at: 0 1195 1428 1449
Size: 1195 233 21
Fragments arranged by size:
1195 233 21
EaeI y'GGCC_r
Cuts at: 0 662 744 1346 1370 1449
Size: 662 82 602 24 79
Fragments arranged by size:
662 602 82 79 24
EarI CTCTTCn'nnn_
Cuts at: 0 202 320 1449
Size: 202 118 1129
Fragments arranged by size:
1129 202 118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 412 617 1036 1253 1358 1449
Size: 193 219 205 419 217 105 91
Fragments arranged by size:
419 219 217 205 193 105 91
EcoO109I rG'GnC_Cy
Cuts at: 0 275 628 1449
Size: 275 353 821
Fragments arranged by size:
821 353 275
EcoRI G'AATT_C
Cuts at: 0 229 1449
Size: 229 1220
HaeII r_GCGC'y
Cuts at: 0 1011 1449
Size: 1011 438
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 892 924 1449
Size: 892 32 525
Fragments arranged by size:
892 525 32
HincII GTy'rAC
Cuts at: 0 717 1449
Size: 717 732
HpaI GTT'AAC
Cuts at: 0 717 1449
Size: 717 732
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 523 569 1449
Size: 184 339 46 880
Fragments arranged by size:
880 339 184 46
MscI TGG'CCA
Cuts at: 0 664 746 1348 1372 1449
Size: 664 82 602 24 77
Fragments arranged by size:
664 602 82 77 24
MslI CAynn'nnrTG
Cuts at: 0 89 616 853 899 1366 1449
Size: 89 527 237 46 467 83
Fragments arranged by size:
527 467 237 89 83 46
MspA1I CmG'CkG
Cuts at: 0 1043 1119 1336 1449
Size: 1043 76 217 113
Fragments arranged by size:
1043 217 113 76
NcoI C'CATG_G
Cuts at: 0 677 851 1449
Size: 677 174 598
Fragments arranged by size:
677 598 174
NheI G'CTAG_C
Cuts at: 0 211 1449
Size: 211 1238
NsiI A_TGCA'T
Cuts at: 0 593 1449
Size: 593 856
NspI r_CATG'y
Cuts at: 0 288 1398 1414 1449
Size: 288 1110 16 35
Fragments arranged by size:
1110 288 35 16
PciI A'CATG_T
Cuts at: 0 284 1449
Size: 284 1165
PshAI GACnn'nnGTC
Cuts at: 0 672 1449
Size: 672 777
PspOMI G'GGCC_C
Cuts at: 0 274 1449
Size: 274 1175
PstI C_TGCA'G
Cuts at: 0 246 1035 1335 1449
Size: 246 789 300 114
Fragments arranged by size:
789 300 246 114
PvuII CAG'CTG
Cuts at: 0 1119 1336 1449
Size: 1119 217 113
Fragments arranged by size:
1119 217 113
SapI GCTCTTCn'nnn_
Cuts at: 0 320 1449
Size: 320 1129
ScaI AGT'ACT
Cuts at: 0 954 1449
Size: 954 495
SfcI C'TryA_G
Cuts at: 0 242 1031 1207 1331 1449
Size: 242 789 176 124 118
Fragments arranged by size:
789 242 176 124 118
SmaI CCC'GGG
Cuts at: 0 35 1449
Size: 35 1414
SmlI C'TyrA_G
Cuts at: 0 1220 1449
Size: 1220 229
SphI G_CATG'C
Cuts at: 0 1398 1449
Size: 1398 51
StyI C'CwwG_G
Cuts at: 0 677 851 1241 1449
Size: 677 174 390 208
Fragments arranged by size:
677 390 208 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 456 1056 1344 1449
Size: 456 600 288 105
Fragments arranged by size:
600 456 288 105
TatI w'GTAC_w
Cuts at: 0 636 952 1449
Size: 636 316 497
Fragments arranged by size:
636 497 316
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 519 551 787 818 819 850 1449
Size: 519 32 236 31 1 31 599
Fragments arranged by size:
599 519 236 32 31 31 1
Tth111I GACn'n_nGTC
Cuts at: 0 130 1449
Size: 130 1319
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 552 1449
Size: 552 897
XmaI C'CCGG_G
Cuts at: 0 33 1449
Size: 33 1416
Enzymes that do cut:
AccI AcuI AfeI AflIII AleI AlfI AlwNI ApaI
ApoI AvaI BanI BanII BciVI BfrBI BlpI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaI BsaBI BsaXI
BseRI BseYI BsgI Bsp1286I BspHI BsrDI BstEII BstXI
BstYI BtgI BtsI DrdI EaeI EarI Eco57MI EcoO109I
EcoRI HaeII Hin4I HincII HpaI MmeI MscI MslI
MspA1I NcoI NheI NsiI NspI PciI PshAI PspOMI
PstI PvuII SapI ScaI SfcI SmaI SmlI SphI
StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BbvCI BcgI BclI BglI BglII BmgBI Bpu10I
BsaAI BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
EagI EciI EcoICRI EcoNI EcoRV FalI FseI FspI
FspAI HindIII KasI KpnI MfeI MluI NaeI NarI
NdeI NgoMIV NotI NruI PacI PasI PflMI PfoI
PmeI PmlI PpiI PpuMI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI
SwaI XbaI XhoI XmnI ZraI