Restriction map for
pF1KB9737
(Linear) (Six-base) MAPSORT of: KIBB9737.seq Check: 4942 from: 1 to: 1500
KIBB9737.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 618 1500
Size: 618 882
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 1303 1500
Size: 526 777 197
Fragments arranged by size:
777 526 197
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1192 1500
Size: 1192 308
AlwNI CAG_nnn'CTG
Cuts at: 0 1095 1104 1500
Size: 1095 9 396
Fragments arranged by size:
1095 396 9
ApoI r'AATT_y
Cuts at: 0 599 1500
Size: 599 901
AvaI C'yCGr_G
Cuts at: 0 72 1500
Size: 72 1428
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 973 1006 1500
Size: 973 33 494
Fragments arranged by size:
973 494 33
BanI G'GyrC_C
Cuts at: 0 17 148 190 205 1500
Size: 17 131 42 15 1295
Fragments arranged by size:
1295 131 42 17 15
BanII G_rGCy'C
Cuts at: 0 28 959 1157 1500
Size: 28 931 198 343
Fragments arranged by size:
931 343 198 28
BbeI G_GCGC'C
Cuts at: 0 194 1500
Size: 194 1306
BbvCI CC'TCA_GC
Cuts at: 0 88 905 1500
Size: 88 817 595
Fragments arranged by size:
817 595 88
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 217 251 1500
Size: 217 34 1249
Fragments arranged by size:
1249 217 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1441 1500
Size: 1441 59
BglI GCCn_nnn'nGGC
Cuts at: 0 107 1213 1500
Size: 107 1106 287
Fragments arranged by size:
1106 287 107
BlpI GC'TnA_GC
Cuts at: 0 656 1135 1500
Size: 656 479 365
Fragments arranged by size:
656 479 365
Bme1580I G_kGCm'C
Cuts at: 0 151 1259 1349 1435 1500
Size: 151 1108 90 86 65
Fragments arranged by size:
1108 151 90 86 65
BmrI ACTGGGnnnn_n'
Cuts at: 0 98 1500
Size: 98 1402
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 948 1500
Size: 948 552
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 973 1179 1297 1306 1357 1477 1500
Size: 12 961 206 118 9 51 120 23
Fragments arranged by size:
961 206 120 118 51 23 12 9
Bpu10I CC'TnA_GC
Cuts at: 0 88 589 905 1439 1500
Size: 88 501 316 534 61
Fragments arranged by size:
534 501 316 88 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 777 985 1015 1500
Size: 777 208 30 485
Fragments arranged by size:
777 485 208 30
BsaBI GATnn'nnATC
Cuts at: 0 844 1500
Size: 844 656
BsaHI Gr'CG_yC
Cuts at: 0 119 191 1500
Size: 119 72 1309
Fragments arranged by size:
1309 119 72
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 10 40 1500
Size: 10 30 1460
Fragments arranged by size:
1460 30 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 940 1030 1165 1500
Size: 940 90 135 335
Fragments arranged by size:
940 335 135 90
BseYI C'CCAG_C
Cuts at: 0 198 767 1500
Size: 198 569 733
Fragments arranged by size:
733 569 198
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1093 1240 1500
Size: 1093 147 260
Fragments arranged by size:
1093 260 147
BsiEI CG_ry'CG
Cuts at: 0 1208 1500
Size: 1208 292
BsiHKAI G_wGCw'C
Cuts at: 0 932 959 1500
Size: 932 27 541
Fragments arranged by size:
932 541 27
Bsp1286I G_dGCh'C
Cuts at: 0 28 151 932 959 1157 1259 1349 1435
Size: 28 123 781 27 198 102 90 86
Cuts at: 1435 1500
Size: 65
Fragments arranged by size:
781 198 123 102 90 86 65 28
27
BspHI T'CATG_A
Cuts at: 0 479 1500
Size: 479 1021
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 618 977 1112 1471 1500
Size: 618 359 135 359 29
Fragments arranged by size:
618 359 359 135 29
BsrBI CCG'CTC
Cuts at: 0 872 1500
Size: 872 628
BsrDI GCAATG_nn'
Cuts at: 0 323 1500
Size: 323 1177
BsrGI T'GTAC_A
Cuts at: 0 1064 1500
Size: 1064 436
BssSI C'ACGA_G
Cuts at: 0 310 1111 1500
Size: 310 801 389
Fragments arranged by size:
801 389 310
BstAPI GCAn_nnn'nTGC
Cuts at: 0 212 461 1104 1383 1500
Size: 212 249 643 279 117
Fragments arranged by size:
643 279 249 212 117
BstXI CCAn_nnnn'nTGG
Cuts at: 0 165 1500
Size: 165 1335
BstYI r'GATC_y
Cuts at: 0 1074 1500
Size: 1074 426
Bsu36I CC'TnA_GG
Cuts at: 0 698 1500
Size: 698 802
BtsI GCAGTG_nn'
Cuts at: 0 1117 1183 1433 1500
Size: 1117 66 250 67
Fragments arranged by size:
1117 250 67 66
ClaI AT'CG_AT
Cuts at: 0 80 1500
Size: 80 1420
DrdI GACnn_nn'nnGTC
Cuts at: 0 57 1500
Size: 57 1443
EaeI y'GGCC_r
Cuts at: 0 260 419 1205 1486 1500
Size: 260 159 786 281 14
Fragments arranged by size:
786 281 260 159 14
EagI C'GGCC_G
Cuts at: 0 1205 1500
Size: 1205 295
EarI CTCTTCn'nnn_
Cuts at: 0 1276 1500
Size: 1276 224
EcoICRI GAG'CTC
Cuts at: 0 957 1500
Size: 957 543
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 526 973 1179 1297 1303 1306 1357
Size: 12 514 447 206 118 6 3 51
Cuts at: 1357 1477 1500
Size: 120 23
Fragments arranged by size:
514 447 206 120 118 51 23 12
6 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1302 1500
Size: 1302 198
EcoO109I rG'GnC_Cy
Cuts at: 0 714 1035 1101 1500
Size: 714 321 66 399
Fragments arranged by size:
714 399 321 66
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 393 1500
Size: 393 1107
HaeII r_GCGC'y
Cuts at: 0 194 303 1146 1500
Size: 194 109 843 354
Fragments arranged by size:
843 354 194 109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 313 345 1500
Size: 313 32 1155
Fragments arranged by size:
1155 313 32
KasI G'GCGC_C
Cuts at: 0 190 1500
Size: 190 1310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 1500
Size: 140 1360
MscI TGG'CCA
Cuts at: 0 1488 1500
Size: 1488 12
MspA1I CmG'CkG
Cuts at: 0 32 125 242 413 978 1191 1500
Size: 32 93 117 171 565 213 309
Fragments arranged by size:
565 309 213 171 117 93 32
NarI GG'CG_CC
Cuts at: 0 191 1500
Size: 191 1309
NspI r_CATG'y
Cuts at: 0 282 1384 1500
Size: 282 1102 116
Fragments arranged by size:
1102 282 116
PasI CC'CwG_GG
Cuts at: 0 144 804 1500
Size: 144 660 696
Fragments arranged by size:
696 660 144
PflMI CCAn_nnn'nTGG
Cuts at: 0 1298 1500
Size: 1298 202
PfoI T'CCnGG_A
Cuts at: 0 1274 1500
Size: 1274 226
PpuMI rG'GwC_Cy
Cuts at: 0 1101 1500
Size: 1101 399
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 391 423 1500
Size: 391 32 1077
Fragments arranged by size:
1077 391 32
PstI C_TGCA'G
Cuts at: 0 830 977 1100 1124 1247 1500
Size: 830 147 123 24 123 253
Fragments arranged by size:
830 253 147 123 123 24
PvuII CAG'CTG
Cuts at: 0 32 413 978 1500
Size: 32 381 565 522
Fragments arranged by size:
565 522 381 32
SacI G_AGCT'C
Cuts at: 0 959 1500
Size: 959 541
SfcI C'TryA_G
Cuts at: 0 826 973 1096 1120 1230 1243 1500
Size: 826 147 123 24 110 13 257
Fragments arranged by size:
826 257 147 123 110 24 13
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1213 1500
Size: 1213 287
SfoI GGC'GCC
Cuts at: 0 192 1500
Size: 192 1308
SmlI C'TyrA_G
Cuts at: 0 792 1000 1030 1500
Size: 792 208 30 470
Fragments arranged by size:
792 470 208 30
StuI AGG'CCT
Cuts at: 0 373 469 1297 1500
Size: 373 96 828 203
Fragments arranged by size:
828 373 203 96
StyI C'CwwG_G
Cuts at: 0 393 1211 1292 1500
Size: 393 818 81 208
Fragments arranged by size:
818 393 208 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 166 192 758 1416 1500
Size: 166 26 566 658 84
Fragments arranged by size:
658 566 166 84 26
TatI w'GTAC_w
Cuts at: 0 275 407 1064 1500
Size: 275 132 657 436
Fragments arranged by size:
657 436 275 132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 10 42 1500
Size: 10 32 1458
Fragments arranged by size:
1458 32 10
XbaI T'CTAG_A
Cuts at: 0 1077 1500
Size: 1077 423
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 103 246 975 1421 1500
Size: 103 143 729 446 79
Fragments arranged by size:
729 446 143 103 79
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApoI AvaI BaeI BanI
BanII BbeI BbvCI BcgI BciVI BglI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrGI BssSI BstAPI BstXI BstYI Bsu36I
BtsI ClaI DrdI EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I KasI MmeI MscI
MspA1I NarI NspI PasI PflMI PfoI PpuMI PsrI
PstI PvuII SacI SfcI SfiI SfoI SmlI StuI
StyI TaqII TatI TstI XbaI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BamHI BbsI BclI BfrBI BglII BmgBI BmtI
BsaI BsaAI BsaWI BsiWI BsmI BsmBI BspEI BsrFI
BssHII BstBI BstEII BstZ17I BtgI BtgZI CspCI DraI
DraIII EciI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MslI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XhoI XmaI XmnI ZraI