Restriction map for
pF1KB9738
(Linear) (Six-base) MAPSORT of: KIBB9738.seq Check: 907 from: 1 to: 1509
KIBB9738.orf
With 182 enzymes: SgfI *
March 5, 2009 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 194 1509
Size: 194 1315
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1203 1509
Size: 1203 306
AflIII A'CryG_T
Cuts at: 0 30 304 1509
Size: 30 274 1205
Fragments arranged by size:
1205 274 30
AleI CACnn'nnGTG
Cuts at: 0 838 1095 1509
Size: 838 257 414
Fragments arranged by size:
838 414 257
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 856 916 1509
Size: 856 60 593
Fragments arranged by size:
856 593 60
AlwNI CAG_nnn'CTG
Cuts at: 0 74 96 1311 1439 1509
Size: 74 22 1215 128 70
Fragments arranged by size:
1215 128 74 70 22
ApaI G_GGCC'C
Cuts at: 0 461 743 1104 1509
Size: 461 282 361 405
Fragments arranged by size:
461 405 361 282
ApaLI G'TGCA_C
Cuts at: 0 1378 1509
Size: 1378 131
ApoI r'AATT_y
Cuts at: 0 1246 1509
Size: 1246 263
AvaI C'yCGr_G
Cuts at: 0 241 333 736 921 1509
Size: 241 92 403 185 588
Fragments arranged by size:
588 403 241 185 92
BanII G_rGCy'C
Cuts at: 0 461 689 743 851 1010 1104 1509
Size: 461 228 54 108 159 94 405
Fragments arranged by size:
461 405 228 159 108 94 54
BbsI GAAGACnn'nnnn_
Cuts at: 0 1218 1403 1509
Size: 1218 185 106
Fragments arranged by size:
1218 185 106
BbvCI CC'TCA_GC
Cuts at: 0 1432 1509
Size: 1432 77
BglI GCCn_nnn'nGGC
Cuts at: 0 465 1509
Size: 465 1044
BlpI GC'TnA_GC
Cuts at: 0 370 1509
Size: 370 1139
Bme1580I G_kGCm'C
Cuts at: 0 128 461 743 1104 1257 1382 1393 1509
Size: 128 333 282 361 153 125 11 116
Fragments arranged by size:
361 333 282 153 128 125 116 11
BmgBI CAC'GTC
Cuts at: 0 870 1509
Size: 870 639
BmrI ACTGGGnnnn_n'
Cuts at: 0 671 1140 1509
Size: 671 469 369
Fragments arranged by size:
671 469 369
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 67 1509
Size: 67 1442
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 653 695 1096 1509
Size: 535 118 42 401 413
Fragments arranged by size:
535 413 401 118 42
Bpu10I CC'TnA_GC
Cuts at: 0 154 1432 1509
Size: 154 1278 77
Fragments arranged by size:
1278 154 77
BsaAI yAC'GTr
Cuts at: 0 1056 1509
Size: 1056 453
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 612 642 1509
Size: 612 30 867
Fragments arranged by size:
867 612 30
BseYI C'CCAG_C
Cuts at: 0 522 694 730 743 850 954 977 994
Size: 522 172 36 13 107 104 23 17
Cuts at: 994 1173 1318 1509
Size: 179 145 191
Fragments arranged by size:
522 191 179 172 145 107 104 36
23 17 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 816 912 1509
Size: 816 96 597
Fragments arranged by size:
816 597 96
BsiEI CG_ry'CG
Cuts at: 0 347 1509
Size: 347 1162
BsiHKAI G_wGCw'C
Cuts at: 0 1010 1382 1509
Size: 1010 372 127
Fragments arranged by size:
1010 372 127
BsmI GAATG_Cn'
Cuts at: 0 988 1509
Size: 988 521
BsmBI CGTCTCn'nnnn_
Cuts at: 0 715 1509
Size: 715 794
Bsp1286I G_dGCh'C
Cuts at: 0 128 461 689 743 851 1010 1104 1257
Size: 128 333 228 54 108 159 94 153
Cuts at: 1257 1382 1393 1509
Size: 125 11 116
Fragments arranged by size:
333 228 159 153 128 125 116 108
94 54 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 194 972 1509
Size: 194 778 537
Fragments arranged by size:
778 537 194
BsrFI r'CCGG_y
Cuts at: 0 265 1509
Size: 265 1244
BsrGI T'GTAC_A
Cuts at: 0 881 1509
Size: 881 628
BssHII G'CGCG_C
Cuts at: 0 142 169 261 1509
Size: 142 27 92 1248
Fragments arranged by size:
1248 142 92 27
BstAPI GCAn_nnn'nTGC
Cuts at: 0 74 1509
Size: 74 1435
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1097 1293 1509
Size: 1097 196 216
Fragments arranged by size:
1097 216 196
BstYI r'GATC_y
Cuts at: 0 609 1509
Size: 609 900
BtgI C'CryG_G
Cuts at: 0 198 749 773 836 1266 1509
Size: 198 551 24 63 430 243
Fragments arranged by size:
551 430 243 198 63 24
BtsI GCAGTG_nn'
Cuts at: 0 97 292 563 1509
Size: 97 195 271 946
Fragments arranged by size:
946 271 195 97
DraIII CAC_nnn'GTG
Cuts at: 0 360 454 1509
Size: 360 94 1055
Fragments arranged by size:
1055 360 94
DrdI GACnn_nn'nnGTC
Cuts at: 0 958 1222 1509
Size: 958 264 287
Fragments arranged by size:
958 287 264
EaeI y'GGCC_r
Cuts at: 0 208 344 1509
Size: 208 136 1165
Fragments arranged by size:
1165 208 136
EagI C'GGCC_G
Cuts at: 0 344 1509
Size: 344 1165
EarI CTCTTCn'nnn_
Cuts at: 0 1330 1509
Size: 1330 179
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1146 1157 1509
Size: 1146 11 352
Fragments arranged by size:
1146 352 11
EcoICRI GAG'CTC
Cuts at: 0 1008 1509
Size: 1008 501
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 653 695 1096 1203 1509
Size: 535 118 42 401 107 306
Fragments arranged by size:
535 401 306 118 107 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 157 550 1509
Size: 157 393 959
Fragments arranged by size:
959 393 157
EcoO109I rG'GnC_Cy
Cuts at: 0 150 339 426 458 508 540 665 740
Size: 150 189 87 32 50 32 125 75
Cuts at: 740 1308 1509
Size: 568 201
Fragments arranged by size:
568 201 189 150 125 87 75 50
32 32
EcoRI G'AATT_C
Cuts at: 0 1246 1509
Size: 1246 263
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 667 1509
Size: 667 842
FspI TGC'GCA
Cuts at: 0 291 298 1509
Size: 291 7 1211
Fragments arranged by size:
1211 291 7
HaeII r_GCGC'y
Cuts at: 0 830 865 1266 1454 1509
Size: 830 35 401 188 55
Fragments arranged by size:
830 401 188 55 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1393 1425 1509
Size: 1393 32 84
Fragments arranged by size:
1393 84 32
MluI A'CGCG_T
Cuts at: 0 30 304 1509
Size: 30 274 1205
Fragments arranged by size:
1205 274 30
MslI CAynn'nnrTG
Cuts at: 0 485 838 1095 1509
Size: 485 353 257 414
Fragments arranged by size:
485 414 353 257
MspA1I CmG'CkG
Cuts at: 0 71 200 287 436 526 751 775 838
Size: 71 129 87 149 90 225 24 63
Cuts at: 838 958 1026 1095 1318 1509
Size: 120 68 69 223 191
Fragments arranged by size:
225 223 191 149 129 120 90 87
71 69 68 63 24
NaeI GCC'GGC
Cuts at: 0 267 1509
Size: 267 1242
NgoMIV G'CCGG_C
Cuts at: 0 265 1509
Size: 265 1244
NspI r_CATG'y
Cuts at: 0 490 1509
Size: 490 1019
PasI CC'CwG_GG
Cuts at: 0 463 682 856 1063 1509
Size: 463 219 174 207 446
Fragments arranged by size:
463 446 219 207 174
PflMI CCAn_nnn'nTGG
Cuts at: 0 454 1239 1509
Size: 454 785 270
Fragments arranged by size:
785 454 270
PfoI T'CCnGG_A
Cuts at: 0 630 1010 1509
Size: 630 380 499
Fragments arranged by size:
630 499 380
PmlI CAC'GTG
Cuts at: 0 1056 1509
Size: 1056 453
PpuMI rG'GwC_Cy
Cuts at: 0 150 339 540 1308 1509
Size: 150 189 201 768 201
Fragments arranged by size:
768 201 201 189 150
PspOMI G'GGCC_C
Cuts at: 0 457 739 1100 1509
Size: 457 282 361 409
Fragments arranged by size:
457 409 361 282
PstI C_TGCA'G
Cuts at: 0 92 905 1509
Size: 92 813 604
Fragments arranged by size:
813 604 92
PvuII CAG'CTG
Cuts at: 0 436 526 958 1026 1095 1318 1509
Size: 436 90 432 68 69 223 191
Fragments arranged by size:
436 432 223 191 90 69 68
SacI G_AGCT'C
Cuts at: 0 1010 1509
Size: 1010 499
SacII CC_GC'GG
Cuts at: 0 201 752 776 839 1509
Size: 201 551 24 63 670
Fragments arranged by size:
670 551 201 63 24
SanDI GG'GwC_CC
Cuts at: 0 339 1509
Size: 339 1170
SapI GCTCTTCn'nnn_
Cuts at: 0 1330 1509
Size: 1330 179
SfcI C'TryA_G
Cuts at: 0 88 901 1509
Size: 88 813 608
Fragments arranged by size:
813 608 88
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 465 1509
Size: 465 1044
SmaI CCC'GGG
Cuts at: 0 738 1509
Size: 738 771
StuI AGG'CCT
Cuts at: 0 184 1509
Size: 184 1325
StyI C'CwwG_G
Cuts at: 0 5 54 623 1328 1509
Size: 5 49 569 705 181
Fragments arranged by size:
705 569 181 49 5
TatI w'GTAC_w
Cuts at: 0 881 1127 1509
Size: 881 246 382
Fragments arranged by size:
881 382 246
Tth111I GACn'n_nGTC
Cuts at: 0 594 1509
Size: 594 915
XmaI C'CCGG_G
Cuts at: 0 736 1509
Size: 736 773
XmnI GAAnn'nnTTC
Cuts at: 0 136 650 1509
Size: 136 514 859
Fragments arranged by size:
859 514 136
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApaI ApaLI
ApoI AvaI BanII BbsI BbvCI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaAI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrFI
BsrGI BssHII BstAPI BstXI BstYI BtgI BtsI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I MluI MslI
MspA1I NaeI NgoMIV NspI PasI PflMI PfoI PmlI
PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI
SfcI SfiI SmaI StuI StyI TatI Tth111I XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI AscI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BcgI BciVI BclI BfrBI BglII BmtI BpuEI
BsaI BsaBI BsaHI BsaWI BseRI BsiWI BspEI BspHI
BsrBI BsrDI BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI EcoRV FseI FspAI HincII HindIII
HpaI KasI KpnI MfeI MmeI MscI NarI NcoI
NdeI NheI NotI NruI NsiI PacI PciI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI
SbfI ScaI SexAI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TstI XbaI
XcmI XhoI ZraI