Restriction map for
pF1KB9744
(Linear) (Six-base) MAPSORT of: KIBB9744.seq Check: 8694 from: 1 to: 1596
KIBB9744.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1369 1596
Size: 1369 227
AatII G_ACGT'C
Cuts at: 0 1523 1596
Size: 1523 73
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 577 661 847 889 1596
Size: 487 90 84 186 42 707
Fragments arranged by size:
707 487 186 90 84 42
AleI CACnn'nnGTG
Cuts at: 0 1567 1596
Size: 1567 29
AlwNI CAG_nnn'CTG
Cuts at: 0 447 1050 1162 1596
Size: 447 603 112 434
Fragments arranged by size:
603 447 434 112
ApaI G_GGCC'C
Cuts at: 0 585 979 1596
Size: 585 394 617
Fragments arranged by size:
617 585 394
ApaLI G'TGCA_C
Cuts at: 0 769 1596
Size: 769 827
AvaI C'yCGr_G
Cuts at: 0 153 401 460 856 1096 1596
Size: 153 248 59 396 240 500
Fragments arranged by size:
500 396 248 240 153 59
BamHI G'GATC_C
Cuts at: 0 471 1596
Size: 471 1125
BanI G'GyrC_C
Cuts at: 0 994 1005 1016 1257 1596
Size: 994 11 11 241 339
Fragments arranged by size:
994 339 241 11 11
BanII G_rGCy'C
Cuts at: 0 585 763 907 979 1596
Size: 585 178 144 72 617
Fragments arranged by size:
617 585 178 144 72
BbeI G_GCGC'C
Cuts at: 0 1261 1596
Size: 1261 335
BbsI GAAGACnn'nnnn_
Cuts at: 0 24 537 565 1596
Size: 24 513 28 1031
Fragments arranged by size:
1031 513 28 24
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1479 1507 1513 1541 1596
Size: 1479 28 6 28 55
Fragments arranged by size:
1479 55 28 28 6
BglI GCCn_nnn'nGGC
Cuts at: 0 658 743 1201 1351 1596
Size: 658 85 458 150 245
Fragments arranged by size:
658 458 245 150 85
BlpI GC'TnA_GC
Cuts at: 0 10 1051 1596
Size: 10 1041 545
Fragments arranged by size:
1041 545 10
Bme1580I G_kGCm'C
Cuts at: 0 585 773 979 1021 1174 1596
Size: 585 188 206 42 153 422
Fragments arranged by size:
585 422 206 188 153 42
BmgBI CAC'GTC
Cuts at: 0 341 1596
Size: 341 1255
BmrI ACTGGGnnnn_n'
Cuts at: 0 415 668 1184 1304 1596
Size: 415 253 516 120 292
Fragments arranged by size:
516 415 292 253 120
BmtI G_CTAG'C
Cuts at: 0 1243 1596
Size: 1243 353
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 893 1042 1596
Size: 100 793 149 554
Fragments arranged by size:
793 554 149 100
Bpu10I CC'TnA_GC
Cuts at: 0 52 1596
Size: 52 1544
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 1596
Size: 268 1328
BsaI GGTCTCn'nnnn_
Cuts at: 0 406 1111 1596
Size: 406 705 485
Fragments arranged by size:
705 485 406
BsaAI yAC'GTr
Cuts at: 0 1506 1596
Size: 1506 90
BsaHI Gr'CG_yC
Cuts at: 0 1258 1520 1596
Size: 1258 262 76
Fragments arranged by size:
1258 262 76
BsaWI w'CCGG_w
Cuts at: 0 206 479 782 1596
Size: 206 273 303 814
Fragments arranged by size:
814 303 273 206
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1399 1429 1596
Size: 1399 30 167
Fragments arranged by size:
1399 167 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 151 318 339 1399 1596
Size: 19 132 167 21 1060 197
Fragments arranged by size:
1060 197 167 132 21 19
BseYI C'CCAG_C
Cuts at: 0 125 894 934 1012 1028 1155 1429 1596
Size: 125 769 40 78 16 127 274 167
Fragments arranged by size:
769 274 167 127 125 78 40 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 1596
Size: 220 1376
BsiEI CG_ry'CG
Cuts at: 0 738 1596
Size: 738 858
BsiHKAI G_wGCw'C
Cuts at: 0 763 773 1505 1596
Size: 763 10 732 91
Fragments arranged by size:
763 732 91 10
Bsp1286I G_dGCh'C
Cuts at: 0 585 763 773 907 979 1021 1174 1505
Size: 585 178 10 134 72 42 153 331
Cuts at: 1505 1596
Size: 91
Fragments arranged by size:
585 331 178 153 134 91 72 42
10
BspEI T'CCGG_A
Cuts at: 0 479 1596
Size: 479 1117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 18 806 1369 1596
Size: 18 788 563 227
Fragments arranged by size:
788 563 227 18
BsrFI r'CCGG_y
Cuts at: 0 233 269 1596
Size: 233 36 1327
Fragments arranged by size:
1327 233 36
BstXI CCAn_nnnn'nTGG
Cuts at: 0 681 819 1254 1569 1596
Size: 681 138 435 315 27
Fragments arranged by size:
681 435 315 138 27
BstYI r'GATC_y
Cuts at: 0 443 471 573 1596
Size: 443 28 102 1023
Fragments arranged by size:
1023 443 102 28
Bsu36I CC'TnA_GG
Cuts at: 0 1585 1596
Size: 1585 11
BtgI C'CryG_G
Cuts at: 0 680 990 1596
Size: 680 310 606
Fragments arranged by size:
680 606 310
BtsI GCAGTG_nn'
Cuts at: 0 248 1259 1305 1360 1523 1596
Size: 248 1011 46 55 163 73
Fragments arranged by size:
1011 248 163 73 55 46
DraIII CAC_nnn'GTG
Cuts at: 0 423 1596
Size: 423 1173
EaeI y'GGCC_r
Cuts at: 0 235 452 545 587 683 701 722 735
Size: 235 217 93 42 96 18 21 13
Cuts at: 735 1145 1355 1596
Size: 410 210 241
Fragments arranged by size:
410 241 235 217 210 96 93 42
21 18 13
EagI C'GGCC_G
Cuts at: 0 735 1596
Size: 735 861
EarI CTCTTCn'nnn_
Cuts at: 0 205 336 357 359 1596
Size: 205 131 21 2 1237
Fragments arranged by size:
1237 205 131 21 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1407 1596
Size: 1407 189
EcoICRI GAG'CTC
Cuts at: 0 761 1596
Size: 761 835
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 487 577 661 847 889 893 1042
Size: 100 387 90 84 186 42 4 149
Cuts at: 1042 1596
Size: 554
Fragments arranged by size:
554 387 186 149 100 90 84 42
4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 547 712 1596
Size: 547 165 884
Fragments arranged by size:
884 547 165
EcoO109I rG'GnC_Cy
Cuts at: 0 397 581 582 930 975 976 1024 1596
Size: 397 184 1 348 45 1 48 572
Fragments arranged by size:
572 397 348 184 48 45 1 1
FseI GG_CCGG'CC
Cuts at: 0 237 1596
Size: 237 1359
HaeII r_GCGC'y
Cuts at: 0 276 617 826 1261 1596
Size: 276 341 209 435 335
Fragments arranged by size:
435 341 335 276 209
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 286 318 1399 1405 1431 1437 1596
Size: 286 32 1081 6 26 6 159
Fragments arranged by size:
1081 286 159 32 26 6 6
KasI G'GCGC_C
Cuts at: 0 1257 1596
Size: 1257 339
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1430 1596
Size: 1430 166
MscI TGG'CCA
Cuts at: 0 454 547 685 703 724 1596
Size: 454 93 138 18 21 872
Fragments arranged by size:
872 454 138 93 21 18
MslI CAynn'nnrTG
Cuts at: 0 253 989 1567 1596
Size: 253 736 578 29
Fragments arranged by size:
736 578 253 29
MspA1I CmG'CkG
Cuts at: 0 125 752 938 1199 1596
Size: 125 627 186 261 397
Fragments arranged by size:
627 397 261 186 125
NaeI GCC'GGC
Cuts at: 0 235 271 1596
Size: 235 36 1325
Fragments arranged by size:
1325 235 36
NarI GG'CG_CC
Cuts at: 0 1258 1596
Size: 1258 338
NcoI C'CATG_G
Cuts at: 0 680 990 1596
Size: 680 310 606
Fragments arranged by size:
680 606 310
NgoMIV G'CCGG_C
Cuts at: 0 233 269 1596
Size: 233 36 1327
Fragments arranged by size:
1327 233 36
NheI G'CTAG_C
Cuts at: 0 1239 1596
Size: 1239 357
NspI r_CATG'y
Cuts at: 0 1095 1596
Size: 1095 501
PasI CC'CwG_GG
Cuts at: 0 1162 1596
Size: 1162 434
PflMI CCAn_nnn'nTGG
Cuts at: 0 447 1065 1162 1596
Size: 447 618 97 434
Fragments arranged by size:
618 447 434 97
PfoI T'CCnGG_A
Cuts at: 0 146 909 1596
Size: 146 763 687
Fragments arranged by size:
763 687 146
PpuMI rG'GwC_Cy
Cuts at: 0 397 1596
Size: 397 1199
PspOMI G'GGCC_C
Cuts at: 0 581 975 1596
Size: 581 394 621
Fragments arranged by size:
621 581 394
PstI C_TGCA'G
Cuts at: 0 268 1596
Size: 268 1328
PvuII CAG'CTG
Cuts at: 0 125 752 1596
Size: 125 627 844
Fragments arranged by size:
844 627 125
SacI G_AGCT'C
Cuts at: 0 763 1596
Size: 763 833
SanDI GG'GwC_CC
Cuts at: 0 397 1596
Size: 397 1199
SexAI A'CCwGG_T
Cuts at: 0 86 1596
Size: 86 1510
SfcI C'TryA_G
Cuts at: 0 264 1596
Size: 264 1332
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1201 1596
Size: 1201 395
SfoI GGC'GCC
Cuts at: 0 1259 1596
Size: 1259 337
SmaI CCC'GGG
Cuts at: 0 403 1098 1596
Size: 403 695 498
Fragments arranged by size:
695 498 403
SmlI C'TyrA_G
Cuts at: 0 283 1596
Size: 283 1313
SphI G_CATG'C
Cuts at: 0 1095 1596
Size: 1095 501
StuI AGG'CCT
Cuts at: 0 629 661 1596
Size: 629 32 935
Fragments arranged by size:
935 629 32
StyI C'CwwG_G
Cuts at: 0 238 548 653 680 990 1133 1596
Size: 238 310 105 27 310 143 463
Fragments arranged by size:
463 310 310 238 143 105 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 434 1003 1004 1053 1057 1278 1596
Size: 434 569 1 49 4 221 318
Fragments arranged by size:
569 434 318 221 49 4 1
Tth111I GACn'n_nGTC
Cuts at: 0 1288 1596
Size: 1288 308
XmaI C'CCGG_G
Cuts at: 0 401 1096 1596
Size: 401 695 500
Fragments arranged by size:
695 500 401
ZraI GAC'GTC
Cuts at: 0 1521 1596
Size: 1521 75
Enzymes that do cut:
AarI AatII AcuI AleI AlwNI ApaI ApaLI AvaI
BamHI BanI BanII BbeI BbsI BcgI BglI BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspMI BsrFI BstXI BstYI Bsu36I
BtgI BtsI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FseI HaeII Hin4I KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII
SacI SanDI SexAI SfcI SfiI SfoI SmaI SmlI
SphI StuI StyI TaqII Tth111I XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BbvCI BciVI BclI BfrBI BglII BplI BsaBI BsiWI
BsmI BsmBI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DrdI EcoRI EcoRV FalI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI NdeI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SapI SbfI ScaI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TatI
TstI XbaI XcmI XhoI XmnI