Restriction map for
pF1KB9746
(Linear) (Six-base) MAPSORT of: KIBB9746.seq Check: 9599 from: 1 to: 1626
KIBB9746.orf
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AccI GT'mk_AC
Cuts at: 0 102 1626
Size: 102 1524
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 546 882 1134 1302 1626
Size: 546 336 252 168 324
Fragments arranged by size:
546 336 324 252 168
AhdI GACnn_n'nnGTC
Cuts at: 0 143 1235 1626
Size: 143 1092 391
Fragments arranged by size:
1092 391 143
AleI CACnn'nnGTG
Cuts at: 0 353 521 1445 1529 1626
Size: 353 168 924 84 97
Fragments arranged by size:
924 353 168 97 84
ApaLI G'TGCA_C
Cuts at: 0 1156 1626
Size: 1156 470
ApoI r'AATT_y
Cuts at: 0 344 596 680 848 1184 1352 1626
Size: 344 252 84 168 336 168 274
Fragments arranged by size:
344 336 274 252 168 168 84
BamHI G'GATC_C
Cuts at: 0 1016 1626
Size: 1016 610
BbsI GAAGACnn'nnnn_
Cuts at: 0 936 1570 1626
Size: 936 634 56
Fragments arranged by size:
936 634 56
BlpI GC'TnA_GC
Cuts at: 0 321 668 1626
Size: 321 347 958
Fragments arranged by size:
958 347 321
Bme1580I G_kGCm'C
Cuts at: 0 1160 1626
Size: 1160 466
BmrI ACTGGGnnnn_n'
Cuts at: 0 446 1034 1370 1626
Size: 446 588 336 256
Fragments arranged by size:
588 446 336 256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1130 1626
Size: 1130 496
BsaAI yAC'GTr
Cuts at: 0 115 1161 1626
Size: 115 1046 465
Fragments arranged by size:
1046 465 115
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 559 1626
Size: 559 1067
BsiHKAI G_wGCw'C
Cuts at: 0 1160 1626
Size: 1160 466
BsmI GAATG_Cn'
Cuts at: 0 628 889 1626
Size: 628 261 737
Fragments arranged by size:
737 628 261
Bsp1286I G_dGCh'C
Cuts at: 0 1160 1626
Size: 1160 466
BsrDI GCAATG_nn'
Cuts at: 0 385 1626
Size: 385 1241
BsrGI T'GTAC_A
Cuts at: 0 1254 1626
Size: 1254 372
BstAPI GCAn_nnn'nTGC
Cuts at: 0 884 1626
Size: 884 742
BstYI r'GATC_y
Cuts at: 0 1016 1626
Size: 1016 610
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 615 650 1539 1574 1626
Size: 615 35 889 35 52
Fragments arranged by size:
889 615 52 35 35
DraI TTT'AAA
Cuts at: 0 1545 1626
Size: 1545 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 546 882 1130 1134 1302 1626
Size: 546 336 248 4 168 324
Fragments arranged by size:
546 336 324 248 168 4
EcoRI G'AATT_C
Cuts at: 0 344 596 680 848 1352 1626
Size: 344 252 84 168 504 274
Fragments arranged by size:
504 344 274 252 168 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 129 161 227 259 1221 1253 1626
Size: 129 32 66 32 962 32 373
Fragments arranged by size:
962 373 129 66 32 32 32
HindIII A'AGCT_T
Cuts at: 0 473 493 1626
Size: 473 20 1133
Fragments arranged by size:
1133 473 20
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 1626
Size: 2 1624
MslI CAynn'nnrTG
Cuts at: 0 117 185 269 353 369 453 521 605
Size: 117 68 84 84 16 84 68 84
Cuts at: 605 773 1193 1433 1445 1529 1613 1626
Size: 168 420 240 12 84 84 13
Fragments arranged by size:
420 240 168 117 84 84 84 84
84 84 68 68 16 13 12
MspA1I CmG'CkG
Cuts at: 0 1518 1626
Size: 1518 108
NdeI CA'TA_TG
Cuts at: 0 450 1610 1626
Size: 450 1160 16
Fragments arranged by size:
1160 450 16
NspI r_CATG'y
Cuts at: 0 268 1626
Size: 268 1358
PmlI CAC'GTG
Cuts at: 0 115 1626
Size: 115 1511
ScaI AGT'ACT
Cuts at: 0 513 987 1626
Size: 513 474 639
Fragments arranged by size:
639 513 474
SexAI A'CCwGG_T
Cuts at: 0 578 1626
Size: 578 1048
StuI AGG'CCT
Cuts at: 0 307 559 643 811 979 1147 1315 1483
Size: 307 252 84 168 168 168 168 168
Cuts at: 1483 1626
Size: 143
Fragments arranged by size:
307 252 168 168 168 168 168 143
84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 527 1626
Size: 527 1099
TatI w'GTAC_w
Cuts at: 0 417 511 985 1254 1626
Size: 417 94 474 269 372
Fragments arranged by size:
474 417 372 269 94
XmnI GAAnn'nnTTC
Cuts at: 0 559 727 895 1626
Size: 559 168 168 731
Fragments arranged by size:
731 559 168 168
Enzymes that do cut:
AccI AcuI AhdI AleI ApaLI ApoI BamHI BbsI
BlpI Bme1580I BmrI BpmI BsaAI BseRI BsiHKAI BsmI
Bsp1286I BsrDI BsrGI BstAPI BstYI CspCI DraI Eco57MI
EcoRI Hin4I HindIII MmeI MslI MspA1I NdeI NspI
PmlI ScaI SexAI StuI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AlfI AloI AlwNI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BanI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BmgBI BmtI BplI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI
BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI DraIII DrdI EaeI EagI EarI
EciI EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StyI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI