Restriction map for
pF1KB9747
(Linear) (Six-base) MAPSORT of: KIBB9747.seq Check: 9972 from: 1 to: 1635
KIBB9747.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AatII G_ACGT'C
Cuts at: 0 161 1635
Size: 161 1474
AccI GT'mk_AC
Cuts at: 0 1418 1635
Size: 1418 217
Acc65I G'GTAC_C
Cuts at: 0 1024 1635
Size: 1024 611
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 1247 1635
Size: 532 715 388
Fragments arranged by size:
715 532 388
AfeI AGC'GCT
Cuts at: 0 59 1635
Size: 59 1576
AflIII A'CryG_T
Cuts at: 0 650 1253 1635
Size: 650 603 382
Fragments arranged by size:
650 603 382
AleI CACnn'nnGTG
Cuts at: 0 649 1412 1635
Size: 649 763 223
Fragments arranged by size:
763 649 223
AlwNI CAG_nnn'CTG
Cuts at: 0 510 954 1635
Size: 510 444 681
Fragments arranged by size:
681 510 444
ApaI G_GGCC'C
Cuts at: 0 48 55 874 1196 1460 1635
Size: 48 7 819 322 264 175
Fragments arranged by size:
819 322 264 175 48 7
AvaI C'yCGr_G
Cuts at: 0 48 88 141 148 1195 1574 1635
Size: 48 40 53 7 1047 379 61
Fragments arranged by size:
1047 379 61 53 48 40 7
BanI G'GyrC_C
Cuts at: 0 250 883 1024 1635
Size: 250 633 141 611
Fragments arranged by size:
633 611 250 141
BanII G_rGCy'C
Cuts at: 0 48 55 95 227 477 874 1138 1196
Size: 48 7 40 132 250 397 264 58
Cuts at: 1196 1387 1460 1635
Size: 191 73 175
Fragments arranged by size:
397 264 250 191 175 132 73 58
48 40 7
BbvCI CC'TCA_GC
Cuts at: 0 115 961 1635
Size: 115 846 674
Fragments arranged by size:
846 674 115
BclI T'GATC_A
Cuts at: 0 260 1635
Size: 260 1375
BglI GCCn_nnn'nGGC
Cuts at: 0 43 438 1635
Size: 43 395 1197
Fragments arranged by size:
1197 395 43
BlpI GC'TnA_GC
Cuts at: 0 419 1132 1243 1635
Size: 419 713 111 392
Fragments arranged by size:
713 419 392 111
Bme1580I G_kGCm'C
Cuts at: 0 48 55 253 874 927 1196 1460 1635
Size: 48 7 198 621 53 269 264 175
Fragments arranged by size:
621 269 264 198 175 53 48 7
BmgBI CAC'GTC
Cuts at: 0 867 1635
Size: 867 768
BmrI ACTGGGnnnn_n'
Cuts at: 0 1428 1635
Size: 1428 207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 883 1635
Size: 883 752
Bpu10I CC'TnA_GC
Cuts at: 0 115 961 1635
Size: 115 846 674
Fragments arranged by size:
846 674 115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 972 1635
Size: 972 663
BsaI GGTCTCn'nnnn_
Cuts at: 0 501 655 1635
Size: 501 154 980
Fragments arranged by size:
980 501 154
BsaAI yAC'GTr
Cuts at: 0 651 1635
Size: 651 984
BsaBI GATnn'nnATC
Cuts at: 0 262 371 1635
Size: 262 109 1264
Fragments arranged by size:
1264 262 109
BsaHI Gr'CG_yC
Cuts at: 0 158 1635
Size: 158 1477
BsaWI w'CCGG_w
Cuts at: 0 861 1635
Size: 861 774
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 1635
Size: 46 1589
BseYI C'CCAG_C
Cuts at: 0 54 292 707 845 1388 1459 1635
Size: 54 238 415 138 543 71 176
Fragments arranged by size:
543 415 238 176 138 71 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 627 1312 1635
Size: 627 685 323
Fragments arranged by size:
685 627 323
BsiHKAI G_wGCw'C
Cuts at: 0 227 1387 1635
Size: 227 1160 248
Fragments arranged by size:
1160 248 227
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1013 1635
Size: 1013 622
Bsp1286I G_dGCh'C
Cuts at: 0 48 55 95 227 253 477 874 927
Size: 48 7 40 132 26 224 397 53
Cuts at: 927 1138 1196 1387 1460 1635
Size: 211 58 191 73 175
Fragments arranged by size:
397 224 211 191 175 132 73 58
53 48 40 26 7
BspHI T'CATG_A
Cuts at: 0 821 1635
Size: 821 814
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 776 962 1235 1635
Size: 776 186 273 400
Fragments arranged by size:
776 400 273 186
BsrBI CCG'CTC
Cuts at: 0 1378 1452 1635
Size: 1378 74 183
Fragments arranged by size:
1378 183 74
BssSI C'ACGA_G
Cuts at: 0 241 913 1635
Size: 241 672 722
Fragments arranged by size:
722 672 241
BstAPI GCAn_nnn'nTGC
Cuts at: 0 954 1635
Size: 954 681
BstEII G'GTnAC_C
Cuts at: 0 1155 1635
Size: 1155 480
BtgI C'CryG_G
Cuts at: 0 176 580 1357 1635
Size: 176 404 777 278
Fragments arranged by size:
777 404 278 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 358 1635
Size: 358 1277
BtsI GCAGTG_nn'
Cuts at: 0 632 759 1002 1155 1635
Size: 632 127 243 153 480
Fragments arranged by size:
632 480 243 153 127
DrdI GACnn_nn'nnGTC
Cuts at: 0 1266 1635
Size: 1266 369
EaeI y'GGCC_r
Cuts at: 0 543 1635
Size: 543 1092
EarI CTCTTCn'nnn_
Cuts at: 0 1000 1080 1635
Size: 1000 80 555
Fragments arranged by size:
1000 555 80
EcoICRI GAG'CTC
Cuts at: 0 225 1385 1635
Size: 225 1160 250
Fragments arranged by size:
1160 250 225
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 883 1247 1635
Size: 532 351 364 388
Fragments arranged by size:
532 388 364 351
EcoNI CCTnn'n_nnAGG
Cuts at: 0 467 1635
Size: 467 1168
EcoO109I rG'GnC_Cy
Cuts at: 0 15 45 145 871 951 1192 1456 1635
Size: 15 30 100 726 80 241 264 179
Fragments arranged by size:
726 264 241 179 100 80 30 15
HaeII r_GCGC'y
Cuts at: 0 61 109 1466 1635
Size: 61 48 1357 169
Fragments arranged by size:
1357 169 61 48
KpnI G_GTAC'C
Cuts at: 0 1028 1635
Size: 1028 607
MscI TGG'CCA
Cuts at: 0 545 1635
Size: 545 1090
MslI CAynn'nnrTG
Cuts at: 0 202 256 316 649 676 1348 1351 1412
Size: 202 54 60 333 27 672 3 61
Cuts at: 1412 1529 1635
Size: 117 106
Fragments arranged by size:
672 333 202 117 106 61 60 54
27 3
MspA1I CmG'CkG
Cuts at: 0 119 178 492 582 711 1242 1359 1635
Size: 119 59 314 90 129 531 117 276
Fragments arranged by size:
531 314 276 129 119 117 90 59
NspI r_CATG'y
Cuts at: 0 1175 1257 1635
Size: 1175 82 378
Fragments arranged by size:
1175 378 82
PciI A'CATG_T
Cuts at: 0 1253 1635
Size: 1253 382
PmlI CAC'GTG
Cuts at: 0 651 1635
Size: 651 984
PpuMI rG'GwC_Cy
Cuts at: 0 145 951 1635
Size: 145 806 684
Fragments arranged by size:
806 684 145
PspOMI G'GGCC_C
Cuts at: 0 44 51 870 1192 1456 1635
Size: 44 7 819 322 264 179
Fragments arranged by size:
819 322 264 179 44 7
PstI C_TGCA'G
Cuts at: 0 8 788 1150 1635
Size: 8 780 362 485
Fragments arranged by size:
780 485 362 8
PvuII CAG'CTG
Cuts at: 0 492 711 1635
Size: 492 219 924
Fragments arranged by size:
924 492 219
SacI G_AGCT'C
Cuts at: 0 227 1387 1635
Size: 227 1160 248
Fragments arranged by size:
1160 248 227
SacII CC_GC'GG
Cuts at: 0 179 583 1360 1635
Size: 179 404 777 275
Fragments arranged by size:
777 404 275 179
SanDI GG'GwC_CC
Cuts at: 0 145 1635
Size: 145 1490
SfcI C'TryA_G
Cuts at: 0 4 784 1146 1284 1635
Size: 4 780 362 138 351
Fragments arranged by size:
780 362 351 138 4
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 43 1635
Size: 43 1592
SmaI CCC'GGG
Cuts at: 0 50 143 150 1635
Size: 50 93 7 1485
Fragments arranged by size:
1485 93 50 7
SmlI C'TyrA_G
Cuts at: 0 987 1635
Size: 987 648
StuI AGG'CCT
Cuts at: 0 37 1635
Size: 37 1598
StyI C'CwwG_G
Cuts at: 0 737 1494 1635
Size: 737 757 141
Fragments arranged by size:
757 737 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 661 721 901 991 1635
Size: 661 60 180 90 644
Fragments arranged by size:
661 644 180 90 60
TatI w'GTAC_w
Cuts at: 0 1178 1635
Size: 1178 457
Tth111I GACn'n_nGTC
Cuts at: 0 160 355 1635
Size: 160 195 1280
Fragments arranged by size:
1280 195 160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 864 1635
Size: 864 771
XmaI C'CCGG_G
Cuts at: 0 48 141 148 1635
Size: 48 93 7 1487
Fragments arranged by size:
1487 93 48 7
XmnI GAAnn'nnTTC
Cuts at: 0 235 1635
Size: 235 1400
ZraI GAC'GTC
Cuts at: 0 159 1635
Size: 159 1476
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AflIII AleI AlwNI
ApaI AvaI BanI BanII BbvCI BclI BglI BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BssSI BstAPI BstEII BtgI
BtgZI BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I HaeII KpnI MscI MslI MspA1I NspI PciI
PmlI PpuMI PspOMI PstI PvuII SacI SacII SanDI
SfcI SfiI SmaI SmlI StuI StyI TaqII TatI
Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AclI AflII AgeI AhdI AlfI AloI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BcgI BciVI BfrBI BglII BmtI BplI BsaXI
BsiEI BsiWI BsmI BspEI BsrDI BsrFI BsrGI BssHII
BstBI BstXI BstYI BstZ17I Bsu36I ClaI CspCI DraI
DraIII EagI EciI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KasI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI
SbfI ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TstI XbaI XhoI