Restriction map for
pF1KB9749
(Linear) (Six-base) MAPSORT of: KIBB9749.seq Check: 4357 from: 1 to: 1677
KIBB9749.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 356 1677
Size: 356 1321
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1299 1677
Size: 1299 378
AflIII A'CryG_T
Cuts at: 0 189 1677
Size: 189 1488
AhdI GACnn_n'nnGTC
Cuts at: 0 450 1227 1257 1677
Size: 450 777 30 420
Fragments arranged by size:
777 450 420 30
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1558 1677
Size: 1558 119
ApaI G_GGCC'C
Cuts at: 0 815 1677
Size: 815 862
ApaLI G'TGCA_C
Cuts at: 0 12 259 1677
Size: 12 247 1418
Fragments arranged by size:
1418 247 12
AvaI C'yCGr_G
Cuts at: 0 115 839 1040 1290 1426 1677
Size: 115 724 201 250 136 251
Fragments arranged by size:
724 251 250 201 136 115
BanI G'GyrC_C
Cuts at: 0 729 937 1465 1677
Size: 729 208 528 212
Fragments arranged by size:
729 528 212 208
BanII G_rGCy'C
Cuts at: 0 206 640 815 1040 1370 1426 1677
Size: 206 434 175 225 330 56 251
Fragments arranged by size:
434 330 251 225 206 175 56
BbeI G_GCGC'C
Cuts at: 0 733 1677
Size: 733 944
BbsI GAAGACnn'nnnn_
Cuts at: 0 147 526 1677
Size: 147 379 1151
Fragments arranged by size:
1151 379 147
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1255 1289 1677
Size: 1255 34 388
Fragments arranged by size:
1255 388 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1054 1677
Size: 1054 623
Bme1580I G_kGCm'C
Cuts at: 0 16 263 815 1468 1677
Size: 16 247 552 653 209
Fragments arranged by size:
653 552 247 209 16
BmgBI CAC'GTC
Cuts at: 0 797 1677
Size: 797 880
BmrI ACTGGGnnnn_n'
Cuts at: 0 455 1110 1677
Size: 455 655 567
Fragments arranged by size:
655 567 455
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1095 1269 1677
Size: 1095 174 408
Fragments arranged by size:
1095 408 174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 917 1081 1119 1125 1137 1287 1677
Size: 673 244 164 38 6 12 150 390
Fragments arranged by size:
673 390 244 164 150 38 12 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1441 1677
Size: 1441 236
BsaI GGTCTCn'nnnn_
Cuts at: 0 772 1624 1677
Size: 772 852 53
Fragments arranged by size:
852 772 53
BsaHI Gr'CG_yC
Cuts at: 0 548 730 1677
Size: 548 182 947
Fragments arranged by size:
947 548 182
BsaWI w'CCGG_w
Cuts at: 0 392 1508 1677
Size: 392 1116 169
Fragments arranged by size:
1116 392 169
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 214 244 379 409 1078 1108 1123 1153
Size: 214 30 135 30 669 30 15 30
Cuts at: 1153 1603 1633 1677
Size: 450 30 44
Fragments arranged by size:
669 450 214 135 44 30 30 30
30 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 235 292 630 736 945 948 951 954
Size: 235 57 338 106 209 3 3 3
Cuts at: 954 1084 1249 1282 1338 1434 1582 1677
Size: 130 165 33 56 96 148 95
Fragments arranged by size:
338 235 209 165 148 130 106 96
95 57 56 33 3 3 3
BseYI C'CCAG_C
Cuts at: 0 36 696 883 1357 1677
Size: 36 660 187 474 320
Fragments arranged by size:
660 474 320 187 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 1677
Size: 148 1529
BsiEI CG_ry'CG
Cuts at: 0 859 1677
Size: 859 818
BsiHKAI G_wGCw'C
Cuts at: 0 16 263 602 640 933 1677
Size: 16 247 339 38 293 744
Fragments arranged by size:
744 339 293 247 38 16
Bsp1286I G_dGCh'C
Cuts at: 0 16 206 263 602 640 815 933 1040
Size: 16 190 57 339 38 175 118 107
Cuts at: 1040 1370 1426 1468 1677
Size: 330 56 42 209
Fragments arranged by size:
339 330 209 190 175 118 107 57
56 42 38 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 356 1232 1677
Size: 356 876 445
Fragments arranged by size:
876 445 356
BsrBI CCG'CTC
Cuts at: 0 609 1677
Size: 609 1068
BsrDI GCAATG_nn'
Cuts at: 0 127 1677
Size: 127 1550
BsrFI r'CCGG_y
Cuts at: 0 412 707 858 984 1276 1677
Size: 412 295 151 126 292 401
Fragments arranged by size:
412 401 295 292 151 126
BstEII G'GTnAC_C
Cuts at: 0 588 1677
Size: 588 1089
BstXI CCAn_nnnn'nTGG
Cuts at: 0 651 1677
Size: 651 1026
BstYI r'GATC_y
Cuts at: 0 1451 1677
Size: 1451 226
BtgI C'CryG_G
Cuts at: 0 71 108 383 534 1211 1667 1677
Size: 71 37 275 151 677 456 10
Fragments arranged by size:
677 456 275 151 71 37 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 28 1677
Size: 28 1649
DraIII CAC_nnn'GTG
Cuts at: 0 141 588 1677
Size: 141 447 1089
Fragments arranged by size:
1089 447 141
EaeI y'GGCC_r
Cuts at: 0 856 860 986 1575 1670 1677
Size: 856 4 126 589 95 7
Fragments arranged by size:
856 589 126 95 7 4
EagI C'GGCC_G
Cuts at: 0 856 1677
Size: 856 821
EarI CTCTTCn'nnn_
Cuts at: 0 898 1260 1621 1677
Size: 898 362 361 56
Fragments arranged by size:
898 362 361 56
EcoICRI GAG'CTC
Cuts at: 0 638 1677
Size: 638 1039
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 917 1081 1119 1125 1137 1287 1299
Size: 673 244 164 38 6 12 150 12
Cuts at: 1299 1677
Size: 378
Fragments arranged by size:
673 378 244 164 150 38 12 12
6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1459 1677
Size: 1459 218
EcoO109I rG'GnC_Cy
Cuts at: 0 812 920 1056 1198 1221 1677
Size: 812 108 136 142 23 456
Fragments arranged by size:
812 456 142 136 108 23
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 171 312 384 765 1677
Size: 171 141 72 381 912
Fragments arranged by size:
912 381 171 141 72
FseI GG_CCGG'CC
Cuts at: 0 862 1677
Size: 862 815
FspI TGC'GCA
Cuts at: 0 58 1642 1677
Size: 58 1584 35
Fragments arranged by size:
1584 58 35
HaeII r_GCGC'y
Cuts at: 0 733 890 1343 1525 1677
Size: 733 157 453 182 152
Fragments arranged by size:
733 453 182 157 152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 436 468 1078 1110 1141 1173 1213 1243
Size: 436 32 610 32 31 32 40 30
Cuts at: 1243 1245 1275 1542 1574 1603 1635 1677
Size: 2 30 267 32 29 32 42
Fragments arranged by size:
610 436 267 42 40 32 32 32
32 32 31 30 30 29 2
KasI G'GCGC_C
Cuts at: 0 729 1677
Size: 729 948
MluI A'CGCG_T
Cuts at: 0 189 1677
Size: 189 1488
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1100 1154 1265 1677
Size: 1100 54 111 412
Fragments arranged by size:
1100 412 111 54
MscI TGG'CCA
Cuts at: 0 1577 1672 1677
Size: 1577 95 5
Fragments arranged by size:
1577 95 5
MslI CAynn'nnrTG
Cuts at: 0 256 1583 1677
Size: 256 1327 94
Fragments arranged by size:
1327 256 94
MspA1I CmG'CkG
Cuts at: 0 110 1677
Size: 110 1567
NaeI GCC'GGC
Cuts at: 0 414 709 860 986 1278 1677
Size: 414 295 151 126 292 399
Fragments arranged by size:
414 399 295 292 151 126
NarI GG'CG_CC
Cuts at: 0 730 1677
Size: 730 947
NcoI C'CATG_G
Cuts at: 0 1211 1677
Size: 1211 466
NgoMIV G'CCGG_C
Cuts at: 0 412 707 858 984 1276 1677
Size: 412 295 151 126 292 401
Fragments arranged by size:
412 401 295 292 151 126
PfoI T'CCnGG_A
Cuts at: 0 922 1677
Size: 922 755
PpuMI rG'GwC_Cy
Cuts at: 0 920 1198 1221 1677
Size: 920 278 23 456
Fragments arranged by size:
920 456 278 23
PspOMI G'GGCC_C
Cuts at: 0 811 1677
Size: 811 866
PstI C_TGCA'G
Cuts at: 0 1229 1438 1547 1652 1677
Size: 1229 209 109 105 25
Fragments arranged by size:
1229 209 109 105 25
SacI G_AGCT'C
Cuts at: 0 640 1677
Size: 640 1037
SacII CC_GC'GG
Cuts at: 0 111 1677
Size: 111 1566
SanDI GG'GwC_CC
Cuts at: 0 920 1677
Size: 920 757
SapI GCTCTTCn'nnn_
Cuts at: 0 898 1677
Size: 898 779
SfcI C'TryA_G
Cuts at: 0 1225 1434 1543 1648 1677
Size: 1225 209 109 105 29
Fragments arranged by size:
1225 209 109 105 29
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1054 1677
Size: 1054 623
SfoI GGC'GCC
Cuts at: 0 731 1677
Size: 731 946
SmaI CCC'GGG
Cuts at: 0 841 1042 1292 1677
Size: 841 201 250 385
Fragments arranged by size:
841 385 250 201
SmlI C'TyrA_G
Cuts at: 0 1456 1677
Size: 1456 221
StuI AGG'CCT
Cuts at: 0 1093 1480 1677
Size: 1093 387 197
Fragments arranged by size:
1093 387 197
StyI C'CwwG_G
Cuts at: 0 542 593 644 677 716 740 803 989
Size: 542 51 51 33 39 24 63 186
Cuts at: 989 1194 1211 1677
Size: 205 17 466
Fragments arranged by size:
542 466 205 186 63 51 51 39
33 24 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 1677
Size: 27 1650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1056 1677
Size: 1056 621
XmaI C'CCGG_G
Cuts at: 0 839 1040 1290 1677
Size: 839 201 250 387
Fragments arranged by size:
839 387 250 201
Enzymes that do cut:
AarI AcuI AflIII AhdI AlfI ApaI ApaLI AvaI
BanI BanII BbeI BbsI BcgI BglI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrDI BsrFI BstEII BstXI BstYI BtgI BtgZI DraIII
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
FseI FspI HaeII Hin4I KasI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV PfoI PpuMI
PspOMI PstI SacI SacII SanDI SapI SfcI SfiI
SfoI SmaI SmlI StuI StyI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AleI
AloI AlwNI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BciVI BclI BfrBI BglII BlpI BmtI
Bpu10I BsaAI BsaBI BsiWI BsmI BsmBI BspEI BspHI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtsI
ClaI CspCI DraI DrdI EciI EcoRI EcoRV FspAI
HincII HindIII HpaI KpnI MfeI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SalI SbfI ScaI SexAI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TatI Tth111I
XbaI XhoI XmnI ZraI