Restriction map for
pF1KB9760
(Linear) (Six-base) MAPSORT of: KIBB9760.seq Check: 201 from: 1 to: 2217
KIBB9760.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 258 2217
Size: 258 1959
AccI GT'mk_AC
Cuts at: 0 614 2217
Size: 614 1603
Acc65I G'GTAC_C
Cuts at: 0 1359 2217
Size: 1359 858
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1780 2217
Size: 1780 437
AfeI AGC'GCT
Cuts at: 0 631 644 2217
Size: 631 13 1573
Fragments arranged by size:
1573 631 13
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1915 2217
Size: 1915 302
AlwNI CAG_nnn'CTG
Cuts at: 0 172 740 1336 1537 1728 2217
Size: 172 568 596 201 191 489
Fragments arranged by size:
596 568 489 201 191 172
ApaI G_GGCC'C
Cuts at: 0 190 393 525 1024 1072 1224 1271 1478
Size: 190 203 132 499 48 152 47 207
Cuts at: 1478 2217
Size: 739
Fragments arranged by size:
739 499 207 203 190 152 132 48
47
ApaLI G'TGCA_C
Cuts at: 0 1546 2217
Size: 1546 671
AscI GG'CGCG_CC
Cuts at: 0 788 2217
Size: 788 1429
AvaI C'yCGr_G
Cuts at: 0 189 198 208 220 262 439 1323 1377
Size: 189 9 10 12 42 177 884 54
Cuts at: 1377 2217
Size: 840
Fragments arranged by size:
884 840 189 177 54 42 12 10
9
AvrII C'CTAG_G
Cuts at: 0 1839 2217
Size: 1839 378
BamHI G'GATC_C
Cuts at: 0 193 2217
Size: 193 2024
BanI G'GyrC_C
Cuts at: 0 1096 1284 1359 1843 1944 2190 2217
Size: 1096 188 75 484 101 246 27
Fragments arranged by size:
1096 484 246 188 101 75 27
BanII G_rGCy'C
Cuts at: 0 190 393 428 525 907 1024 1072 1224
Size: 190 203 35 97 382 117 48 152
Cuts at: 1224 1271 1402 1478 2207 2217
Size: 47 131 76 729 10
Fragments arranged by size:
729 382 203 190 152 131 117 97
76 48 47 35 10
BbeI G_GCGC'C
Cuts at: 0 1948 2217
Size: 1948 269
BbvCI CC'TCA_GC
Cuts at: 0 182 2080 2159 2217
Size: 182 1898 79 58
Fragments arranged by size:
1898 182 79 58
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 877 911 1629 1663 2217
Size: 877 34 718 34 554
Fragments arranged by size:
877 718 554 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1394 2217
Size: 1394 823
BglI GCCn_nnn'nGGC
Cuts at: 0 311 362 397 520 1228 1326 2217
Size: 311 51 35 123 708 98 891
Fragments arranged by size:
891 708 311 123 98 51 35
Bme1580I G_kGCm'C
Cuts at: 0 190 393 525 1024 1072 1101 1224 1271
Size: 190 203 132 499 48 29 123 47
Cuts at: 1271 1478 1550 2217
Size: 207 72 667
Fragments arranged by size:
667 499 207 203 190 132 123 72
48 47 29
BmrI ACTGGGnnnn_n'
Cuts at: 0 871 1094 2217
Size: 871 223 1123
Fragments arranged by size:
1123 871 223
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 880 1044 1204 1872 2167 2217
Size: 280 600 164 160 668 295 50
Fragments arranged by size:
668 600 295 280 164 160 50
Bpu10I CC'TnA_GC
Cuts at: 0 182 1396 2080 2159 2217
Size: 182 1214 684 79 58
Fragments arranged by size:
1214 684 182 79 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 339 631 2217
Size: 339 292 1586
Fragments arranged by size:
1586 339 292
BsaI GGTCTCn'nnnn_
Cuts at: 0 1129 1204 2217
Size: 1129 75 1013
Fragments arranged by size:
1129 1013 75
BsaAI yAC'GTr
Cuts at: 0 361 2217
Size: 361 1856
BsaBI GATnn'nnATC
Cuts at: 0 815 2217
Size: 815 1402
BsaHI Gr'CG_yC
Cuts at: 0 203 1945 2217
Size: 203 1742 272
Fragments arranged by size:
1742 272 203
BsaWI w'CCGG_w
Cuts at: 0 767 2217
Size: 767 1450
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 915 945 1180 1210 1244 1274 2217
Size: 915 30 235 30 34 30 943
Fragments arranged by size:
943 915 235 34 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 97 100 619 945 1000 1201 1270
Size: 94 3 3 519 326 55 201 69
Cuts at: 1270 2215 2217
Size: 945 2
Fragments arranged by size:
945 519 326 201 94 69 55 3
3 2
BseYI C'CCAG_C
Cuts at: 0 40 999 1114 1810 2052 2177 2217
Size: 40 959 115 696 242 125 40
Fragments arranged by size:
959 696 242 125 115 40 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1687 1838 2217
Size: 1687 151 379
Fragments arranged by size:
1687 379 151
BsiEI CG_ry'CG
Cuts at: 0 74 125 149 315 350 531 768 1982
Size: 74 51 24 166 35 181 237 1214
Cuts at: 1982 2217
Size: 235
Fragments arranged by size:
1214 237 235 181 166 74 51 35
24
BsiHKAI G_wGCw'C
Cuts at: 0 428 1010 1550 2217
Size: 428 582 540 667
Fragments arranged by size:
667 582 540 428
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1722 1762 2217
Size: 1722 40 455
Fragments arranged by size:
1722 455 40
Bsp1286I G_dGCh'C
Cuts at: 0 190 393 428 525 907 1010 1024 1072
Size: 190 203 35 97 382 103 14 48
Cuts at: 1072 1101 1224 1271 1402 1478 1550 2207 2217
Size: 29 123 47 131 76 72 657 10
Fragments arranged by size:
657 382 203 190 131 123 103 97
76 72 48 47 35 29 14 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 258 500 720 1430 2217
Size: 258 242 220 710 787
Fragments arranged by size:
787 710 258 242 220
BsrBI CCG'CTC
Cuts at: 0 519 1365 2217
Size: 519 846 852
Fragments arranged by size:
852 846 519
BsrFI r'CCGG_y
Cuts at: 0 33 310 431 712 752 925 2217
Size: 33 277 121 281 40 173 1292
Fragments arranged by size:
1292 281 277 173 121 40 33
BssHII G'CGCG_C
Cuts at: 0 94 381 383 495 497 499 533 788
Size: 94 287 2 112 2 2 34 255
Cuts at: 788 2217
Size: 1429
Fragments arranged by size:
1429 287 255 112 94 34 2 2
2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 114 172 1537 2217
Size: 114 58 1365 680
Fragments arranged by size:
1365 680 114 58
BstEII G'GTnAC_C
Cuts at: 0 1452 2217
Size: 1452 765
BstXI CCAn_nnnn'nTGG
Cuts at: 0 915 2217
Size: 915 1302
BstYI r'GATC_y
Cuts at: 0 193 2217
Size: 193 2024
Bsu36I CC'TnA_GG
Cuts at: 0 851 1121 1787 2102 2217
Size: 851 270 666 315 115
Fragments arranged by size:
851 666 315 270 115
BtgI C'CryG_G
Cuts at: 0 242 525 660 1388 1976 2217
Size: 242 283 135 728 588 241
Fragments arranged by size:
728 588 283 242 241 135
BtsI GCAGTG_nn'
Cuts at: 0 337 551 1010 1598 2217
Size: 337 214 459 588 619
Fragments arranged by size:
619 588 459 337 214
EaeI y'GGCC_r
Cuts at: 0 122 146 312 347 528 701 1595 1622
Size: 122 24 166 35 181 173 894 27
Cuts at: 1622 1661 1772 1979 2217
Size: 39 111 207 238
Fragments arranged by size:
894 238 207 181 173 166 122 111
39 35 27 24
EagI C'GGCC_G
Cuts at: 0 122 146 312 347 528 1979 2217
Size: 122 24 166 35 181 1451 238
Fragments arranged by size:
1451 238 181 166 122 35 24
EarI CTCTTCn'nnn_
Cuts at: 0 71 2217
Size: 71 2146
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 873 2217
Size: 873 1344
EcoICRI GAG'CTC
Cuts at: 0 426 2217
Size: 426 1791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 880 1044 1204 1780 1872 2167 2217
Size: 280 600 164 160 576 92 295 50
Fragments arranged by size:
600 576 295 280 164 160 92 50
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1051 1166 1252 1426 1462 1790 2217
Size: 1051 115 86 174 36 328 427
Fragments arranged by size:
1051 427 328 174 115 86 36
EcoO109I rG'GnC_Cy
Cuts at: 0 186 390 483 855 1020 1021 1203 1220
Size: 186 204 93 372 165 1 182 17
Cuts at: 1220 1260 1267 1268 1474 1475 1534 1725 2064
Size: 40 7 1 206 1 59 191 339
Cuts at: 2064 2217
Size: 153
Fragments arranged by size:
372 339 206 204 191 186 182 165
153 93 59 40 17 7 1 1
1
FspI TGC'GCA
Cuts at: 0 565 2217
Size: 565 1652
HaeII r_GCGC'y
Cuts at: 0 292 357 633 646 696 1948 2217
Size: 292 65 276 13 50 1252 269
Fragments arranged by size:
1252 292 276 269 65 50 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 820 852 915 947 1180 1212 1244 1276
Size: 820 32 63 32 233 32 32 32
Cuts at: 1276 2217
Size: 941
Fragments arranged by size:
941 820 233 63 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2142 2217
Size: 2142 75
KasI G'GCGC_C
Cuts at: 0 1944 2217
Size: 1944 273
KpnI G_GTAC'C
Cuts at: 0 1363 2217
Size: 1363 854
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1178 2132 2217
Size: 1178 954 85
Fragments arranged by size:
1178 954 85
MscI TGG'CCA
Cuts at: 0 703 1624 2217
Size: 703 921 593
Fragments arranged by size:
921 703 593
MslI CAynn'nnrTG
Cuts at: 0 964 1708 1780 2217
Size: 964 744 72 437
Fragments arranged by size:
964 744 437 72
MspA1I CmG'CkG
Cuts at: 0 169 408 527 579 662 895 992 1074
Size: 169 239 119 52 83 233 97 82
Cuts at: 1074 1485 1525 1587 1876 1978 2217
Size: 411 40 62 289 102 239
Fragments arranged by size:
411 289 239 239 233 169 119 102
97 83 82 62 52 40
NaeI GCC'GGC
Cuts at: 0 35 312 2217
Size: 35 277 1905
Fragments arranged by size:
1905 277 35
NarI GG'CG_CC
Cuts at: 0 1945 2217
Size: 1945 272
NgoMIV G'CCGG_C
Cuts at: 0 33 310 2217
Size: 33 277 1907
Fragments arranged by size:
1907 277 33
NotI GC'GGCC_GC
Cuts at: 0 528 2217
Size: 528 1689
NspI r_CATG'y
Cuts at: 0 1713 2217
Size: 1713 504
PasI CC'CwG_GG
Cuts at: 0 900 1047 2217
Size: 900 147 1170
Fragments arranged by size:
1170 900 147
PflMI CCAn_nnn'nTGG
Cuts at: 0 1249 1470 2217
Size: 1249 221 747
Fragments arranged by size:
1249 747 221
PfoI T'CCnGG_A
Cuts at: 0 1154 1236 2217
Size: 1154 82 981
Fragments arranged by size:
1154 981 82
PpuMI rG'GwC_Cy
Cuts at: 0 483 855 1260 1725 2217
Size: 483 372 405 465 492
Fragments arranged by size:
492 483 465 405 372
PspOMI G'GGCC_C
Cuts at: 0 186 389 521 1020 1068 1220 1267 1474
Size: 186 203 132 499 48 152 47 207
Cuts at: 1474 2217
Size: 743
Fragments arranged by size:
743 499 207 203 186 152 132 48
47
PstI C_TGCA'G
Cuts at: 0 482 1484 1530 1586 2217
Size: 482 1002 46 56 631
Fragments arranged by size:
1002 631 482 56 46
PvuII CAG'CTG
Cuts at: 0 1485 1525 1587 2217
Size: 1485 40 62 630
Fragments arranged by size:
1485 630 62 40
RsrII CG'GwC_CG
Cuts at: 0 770 2217
Size: 770 1447
SacI G_AGCT'C
Cuts at: 0 428 2217
Size: 428 1789
SacII CC_GC'GG
Cuts at: 0 528 663 1979 2217
Size: 528 135 1316 238
Fragments arranged by size:
1316 528 238 135
SbfI CC_TGCA'GG
Cuts at: 0 482 2217
Size: 482 1735
SfcI C'TryA_G
Cuts at: 0 478 1480 1526 1582 2217
Size: 478 1002 46 56 635
Fragments arranged by size:
1002 635 478 56 46
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 311 397 520 2217
Size: 311 86 123 1697
Fragments arranged by size:
1697 311 123 86
SfoI GGC'GCC
Cuts at: 0 1946 2217
Size: 1946 271
SmaI CCC'GGG
Cuts at: 0 191 200 210 1325 1379 2217
Size: 191 9 10 1115 54 838
Fragments arranged by size:
1115 838 191 54 10 9
SmlI C'TyrA_G
Cuts at: 0 318 439 646 2217
Size: 318 121 207 1571
Fragments arranged by size:
1571 318 207 121
SphI G_CATG'C
Cuts at: 0 1713 2217
Size: 1713 504
StuI AGG'CCT
Cuts at: 0 652 1057 1796 2217
Size: 652 405 739 421
Fragments arranged by size:
739 652 421 405
StyI C'CwwG_G
Cuts at: 0 1248 1839 2165 2207 2217
Size: 1248 591 326 42 10
Fragments arranged by size:
1248 591 326 42 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 88 2217
Size: 88 2129
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1146 1285 1446 2217
Size: 1146 139 161 771
Fragments arranged by size:
1146 771 161 139
XhoI C'TCGA_G
Cuts at: 0 439 2217
Size: 439 1778
XmaI C'CCGG_G
Cuts at: 0 189 198 208 1323 1377 2217
Size: 189 9 10 1115 54 840
Fragments arranged by size:
1115 840 189 54 10 9
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AlfI AlwNI ApaI
ApaLI AscI AvaI AvrII BamHI BanI BanII BbeI
BbvCI BcgI BciVI BglI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssHII BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I HincII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI
PasI PflMI PfoI PpuMI PspOMI PstI PvuII RsrII
SacI SacII SbfI SfcI SfiI SfoI SmaI SmlI
SphI StuI StyI TaqII XcmI XhoI XmaI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AhdI AleI AloI
ApoI AseI AsiSI BaeI BbsI BclI BfrBI BglII
BlpI BmgBI BmtI BplI BsiWI BsmI BspEI BspHI
BsrDI BsrGI BssSI BstBI BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EcoRI EcoRV FalI FseI FspAI
HindIII HpaI MfeI MluI NcoI NdeI NheI NruI
NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI SalI SanDI SapI ScaI SexAI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TatI
TstI Tth111I XbaI XmnI ZraI