Restriction map for
pF1KB9764
(Linear) (Six-base) MAPSORT of: KIBB9764.seq Check: 4791 from: 1 to: 2517
KIBB9764.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AclI AA'CG_TT
Cuts at: 0 945 2517
Size: 945 1572
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 375 464 547 1632 2517
Size: 375 89 83 1085 885
Fragments arranged by size:
1085 885 375 89 83
AflIII A'CryG_T
Cuts at: 0 898 2462 2517
Size: 898 1564 55
Fragments arranged by size:
1564 898 55
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 118 150 2517
Size: 118 32 2367
Fragments arranged by size:
2367 118 32
ApoI r'AATT_y
Cuts at: 0 46 283 608 816 984 1010 1068 1094
Size: 46 237 325 208 168 26 58 26
Cuts at: 1094 1236 1262 1572 1598 1740 1766 1850 1992
Size: 142 26 310 26 142 26 84 142
Cuts at: 1992 2244 2270 2354 2511 2517
Size: 252 26 84 157 6
Fragments arranged by size:
325 310 252 237 208 168 157 142
142 142 84 84 58 46 26 26
26 26 26 26 6
BanII G_rGCy'C
Cuts at: 0 1152 2517
Size: 1152 1365
BbsI GAAGACnn'nnnn_
Cuts at: 0 1050 1218 1554 1722 1974 2226 2517
Size: 1050 168 336 168 252 252 291
Fragments arranged by size:
1050 336 291 252 252 168 168
BclI T'GATC_A
Cuts at: 0 263 2517
Size: 263 2254
BlpI GC'TnA_GC
Cuts at: 0 1161 2517
Size: 1161 1356
BmgBI CAC'GTC
Cuts at: 0 105 2517
Size: 105 2412
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1040 1124 1278 1292 1460 1544 1628 1712
Size: 1040 84 154 14 168 84 84 84
Cuts at: 1712 1796 1880 2048 2132 2300 2517
Size: 84 84 168 84 168 217
Fragments arranged by size:
1040 217 168 168 168 154 84 84
84 84 84 84 84 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 2517
Size: 442 2075
BsaWI w'CCGG_w
Cuts at: 0 1354 2362 2517
Size: 1354 1008 155
Fragments arranged by size:
1354 1008 155
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 118 148 2517
Size: 118 30 2369
Fragments arranged by size:
2369 118 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 2517
Size: 73 2444
BsiHKAI G_wGCw'C
Cuts at: 0 1152 1488 2517
Size: 1152 336 1029
Fragments arranged by size:
1152 1029 336
BsmI GAATG_Cn'
Cuts at: 0 1387 2517
Size: 1387 1130
Bsp1286I G_dGCh'C
Cuts at: 0 1152 1488 2517
Size: 1152 336 1029
Fragments arranged by size:
1152 1029 336
BspHI T'CATG_A
Cuts at: 0 1377 2517
Size: 1377 1140
BsrBI CCG'CTC
Cuts at: 0 82 2517
Size: 82 2435
BsrDI GCAATG_nn'
Cuts at: 0 2223 2517
Size: 2223 294
BsrGI T'GTAC_A
Cuts at: 0 95 2517
Size: 95 2422
BstXI CCAn_nnnn'nTGG
Cuts at: 0 242 2440 2517
Size: 242 2198 77
Fragments arranged by size:
2198 242 77
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2205 2240 2517
Size: 2205 35 277
Fragments arranged by size:
2205 277 35
EarI CTCTTCn'nnn_
Cuts at: 0 282 310 492 735 2517
Size: 282 28 182 243 1782
Fragments arranged by size:
1782 282 243 182 28
EcoICRI GAG'CTC
Cuts at: 0 1150 2517
Size: 1150 1367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 375 464 547 1040 1124 1278 1292 1460
Size: 375 89 83 493 84 154 14 168
Cuts at: 1460 1544 1628 1632 1712 1796 1880 2048 2132
Size: 84 84 4 80 84 84 168 84
Cuts at: 2132 2300 2517
Size: 168 217
Fragments arranged by size:
493 375 217 168 168 168 154 89
84 84 84 84 84 84 83 80
14 4
EcoRI G'AATT_C
Cuts at: 0 46 1068 1094 1262 1598 1740 1766 1850
Size: 46 1022 26 168 336 142 26 84
Cuts at: 1850 1992 2244 2270 2354 2511 2517
Size: 142 252 26 84 157 6
Fragments arranged by size:
1022 336 252 168 157 142 142 84
84 46 26 26 26 6
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 481 2517
Size: 481 2036
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 255 287 712 744 2517
Size: 255 32 425 32 1773
Fragments arranged by size:
1773 425 255 32 32
HindIII A'AGCT_T
Cuts at: 0 532 1158 2083 2517
Size: 532 626 925 434
Fragments arranged by size:
925 626 532 434
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 158 332 2517
Size: 92 66 174 2185
Fragments arranged by size:
2185 174 92 66
MslI CAynn'nnrTG
Cuts at: 0 1943 2127 2379 2438 2517
Size: 1943 184 252 59 79
Fragments arranged by size:
1943 252 184 79 59
NdeI CA'TA_TG
Cuts at: 0 2124 2376 2517
Size: 2124 252 141
Fragments arranged by size:
2124 252 141
NspI r_CATG'y
Cuts at: 0 71 2517
Size: 71 2446
PasI CC'CwG_GG
Cuts at: 0 12 138 2517
Size: 12 126 2379
Fragments arranged by size:
2379 126 12
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 118 150 2517
Size: 118 32 2367
Fragments arranged by size:
2367 118 32
PsiI TTA'TAA
Cuts at: 0 683 1034 1454 1790 2517
Size: 683 351 420 336 727
Fragments arranged by size:
727 683 420 351 336
PstI C_TGCA'G
Cuts at: 0 587 2517
Size: 587 1930
SacI G_AGCT'C
Cuts at: 0 1152 2517
Size: 1152 1365
SfcI C'TryA_G
Cuts at: 0 41 583 2517
Size: 41 542 1934
Fragments arranged by size:
1934 542 41
SmlI C'TyrA_G
Cuts at: 0 421 2517
Size: 421 2096
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 25 2517
Size: 25 2492
TatI w'GTAC_w
Cuts at: 0 95 216 2517
Size: 95 121 2301
Fragments arranged by size:
2301 121 95
XmnI GAAnn'nnTTC
Cuts at: 0 637 900 2233 2517
Size: 637 263 1333 284
Fragments arranged by size:
1333 637 284 263
Enzymes that do cut:
AclI AcuI AflIII AloI ApoI BanII BbsI BclI
BlpI BmgBI BpmI BpuEI BsaWI BsaXI BseRI BsiHKAI
BsmI Bsp1286I BspHI BsrBI BsrDI BsrGI BstXI CspCI
EarI EcoICRI Eco57MI EcoRI FalI Hin4I HindIII MmeI
MslI NdeI NspI PasI PpiI PsiI PstI SacI
SfcI SmlI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BfrBI BglI BglII Bme1580I BmrI BmtI BplI
Bpu10I BsaI BsaAI BsaBI BsaHI BseYI BsgI BsiEI
BsiWI BsmBI BspEI BspMI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EciI EcoNI
EcoO109I EcoRV FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI MscI MspA1I NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI PacI PciI
PflMI PfoI PmeI PmlI PpuMI PshAI PspOMI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI