Restriction map for
pF1KB9772
(Linear) (Six-base) MAPSORT of: KIBB9772.seq Check: 3816 from: 1 to: 1647
KIBB9772.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 0 338 606 1647
Size: 338 268 1041
Fragments arranged by size:
1041 338 268
AclI AA'CG_TT
Cuts at: 0 381 1647
Size: 381 1266
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 142 396 496 1485 1569 1647
Size: 101 41 254 100 989 84 78
Fragments arranged by size:
989 254 101 100 84 78 41
AflII C'TTAA_G
Cuts at: 0 277 846 1647
Size: 277 569 801
Fragments arranged by size:
801 569 277
AflIII A'CryG_T
Cuts at: 0 285 1393 1429 1647
Size: 285 1108 36 218
Fragments arranged by size:
1108 285 218 36
ApaLI G'TGCA_C
Cuts at: 0 447 1647
Size: 447 1200
ApoI r'AATT_y
Cuts at: 0 18 1002 1031 1283 1647
Size: 18 984 29 252 364
Fragments arranged by size:
984 364 252 29 18
AseI AT'TA_AT
Cuts at: 0 497 1647
Size: 497 1150
BamHI G'GATC_C
Cuts at: 0 963 1451 1647
Size: 963 488 196
Fragments arranged by size:
963 488 196
BclI T'GATC_A
Cuts at: 0 1020 1647
Size: 1020 627
BglII A'GATC_T
Cuts at: 0 919 1647
Size: 919 728
Bme1580I G_kGCm'C
Cuts at: 0 451 1647
Size: 451 1196
BmrI ACTGGGnnnn_n'
Cuts at: 0 47 1647
Size: 47 1600
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 201 562 1647
Size: 201 361 1085
Fragments arranged by size:
1085 361 201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 171 243 392 725 809 1229 1313
Size: 25 146 72 149 333 84 420 84
Cuts at: 1313 1329 1481 1565 1647
Size: 16 152 84 82
Fragments arranged by size:
420 333 152 149 146 84 84 84
82 72 25 16
BsaAI yAC'GTr
Cuts at: 0 1394 1430 1647
Size: 1394 36 217
Fragments arranged by size:
1394 217 36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 212 242 1647
Size: 212 30 1405
Fragments arranged by size:
1405 212 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 337 1647
Size: 115 222 1310
Fragments arranged by size:
1310 222 115
BsiHKAI G_wGCw'C
Cuts at: 0 451 1647
Size: 451 1196
BsmI GAATG_Cn'
Cuts at: 0 895 1338 1647
Size: 895 443 309
Fragments arranged by size:
895 443 309
Bsp1286I G_dGCh'C
Cuts at: 0 451 1647
Size: 451 1196
BspHI T'CATG_A
Cuts at: 0 1611 1647
Size: 1611 36
BsrDI GCAATG_nn'
Cuts at: 0 250 1647
Size: 250 1397
BstBI TT'CG_AA
Cuts at: 0 1029 1605 1647
Size: 1029 576 42
Fragments arranged by size:
1029 576 42
BstYI r'GATC_y
Cuts at: 0 919 963 1171 1451 1647
Size: 919 44 208 280 196
Fragments arranged by size:
919 280 208 196 44
BstZ17I GTA'TAC
Cuts at: 0 607 1647
Size: 607 1040
BtsI GCAGTG_nn'
Cuts at: 0 480 953 1647
Size: 480 473 694
Fragments arranged by size:
694 480 473
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 576 611 882 917 1647
Size: 576 35 271 35 730
Fragments arranged by size:
730 576 271 35 35
DraI TTT'AAA
Cuts at: 0 944 1647
Size: 944 703
EaeI y'GGCC_r
Cuts at: 0 23 1647
Size: 23 1624
EarI CTCTTCn'nnn_
Cuts at: 0 49 74 1647
Size: 49 25 1573
Fragments arranged by size:
1573 49 25
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 101 142 171 243 392 396 496
Size: 25 76 41 29 72 149 4 100
Cuts at: 496 725 809 1229 1313 1329 1481 1485 1565
Size: 229 84 420 84 16 152 4 80
Cuts at: 1565 1569 1647
Size: 4 78
Fragments arranged by size:
420 229 152 149 100 84 84 80
78 76 72 41 29 25 16 4
4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 160 747 1647
Size: 160 587 900
Fragments arranged by size:
900 587 160
EcoO109I rG'GnC_Cy
Cuts at: 0 1133 1647
Size: 1133 514
EcoRI G'AATT_C
Cuts at: 0 1031 1283 1647
Size: 1031 252 364
Fragments arranged by size:
1031 364 252
EcoRV GAT'ATC
Cuts at: 0 37 1647
Size: 37 1610
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 151 984 1647
Size: 151 833 663
Fragments arranged by size:
833 663 151
HaeII r_GCGC'y
Cuts at: 0 1379 1647
Size: 1379 268
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 58 212 244 1624 1647
Size: 26 32 154 32 1380 23
Fragments arranged by size:
1380 154 32 32 26 23
HincII GTy'rAC
Cuts at: 0 760 853 1437 1647
Size: 760 93 584 210
Fragments arranged by size:
760 584 210 93
HindIII A'AGCT_T
Cuts at: 0 113 461 1647
Size: 113 348 1186
Fragments arranged by size:
1186 348 113
HpaI GTT'AAC
Cuts at: 0 1437 1647
Size: 1437 210
MscI TGG'CCA
Cuts at: 0 25 1647
Size: 25 1622
MslI CAynn'nnrTG
Cuts at: 0 318 872 1392 1396 1628 1647
Size: 318 554 520 4 232 19
Fragments arranged by size:
554 520 318 232 19 4
NdeI CA'TA_TG
Cuts at: 0 857 1647
Size: 857 790
NspI r_CATG'y
Cuts at: 0 289 1111 1365 1449 1627 1647
Size: 289 822 254 84 178 20
Fragments arranged by size:
822 289 254 178 84 20
PciI A'CATG_T
Cuts at: 0 285 1647
Size: 285 1362
PmlI CAC'GTG
Cuts at: 0 1394 1430 1647
Size: 1394 36 217
Fragments arranged by size:
1394 217 36
PsiI TTA'TAA
Cuts at: 0 803 1647
Size: 803 844
SapI GCTCTTCn'nnn_
Cuts at: 0 49 1647
Size: 49 1598
SexAI A'CCwGG_T
Cuts at: 0 748 1647
Size: 748 899
SmlI C'TyrA_G
Cuts at: 0 277 846 1647
Size: 277 569 801
Fragments arranged by size:
801 569 277
SphI G_CATG'C
Cuts at: 0 1111 1627 1647
Size: 1111 516 20
Fragments arranged by size:
1111 516 20
SspI AAT'ATT
Cuts at: 0 684 1647
Size: 684 963
StuI AGG'CCT
Cuts at: 0 1162 1647
Size: 1162 485
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 862 1647
Size: 862 785
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 549 581 1647
Size: 549 32 1066
Fragments arranged by size:
1066 549 32
XmnI GAAnn'nnTTC
Cuts at: 0 490 742 1078 1498 1582 1647
Size: 490 252 336 420 84 65
Fragments arranged by size:
490 420 336 252 84 65
Enzymes that do cut:
AccI AclI AcuI AflII AflIII ApaLI ApoI AseI
BamHI BclI BglII Bme1580I BmrI BplI BpmI BsaAI
BsaXI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BstBI
BstYI BstZ17I BtsI CspCI DraI EaeI EarI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII
HindIII HpaI MscI MslI NdeI NspI PciI PmlI
PsiI SapI SexAI SmlI SphI SspI StuI TaqII
TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AgeI AhdI AleI AlfI
AloI AlwNI ApaI AscI AsiSI AvaI AvrII BaeI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BfrBI
BglI BlpI BmgBI BmtI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiWI BsmBI BspEI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstXI Bsu36I BtgI BtgZI ClaI DraIII DrdI EagI
EciI EcoICRI FseI FspI FspAI KasI KpnI MfeI
MluI MmeI MspA1I NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI StyI SwaI TatI Tth111I XbaI XcmI XhoI
XmaI ZraI