Restriction map for
pF1KB9773
(Linear) (Six-base) MAPSORT of: KIBB9773.seq Check: 2271 from: 1 to: 1923
KIBB9773.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 300 1791 1923
Size: 300 1491 132
Fragments arranged by size:
1491 300 132
AflIII A'CryG_T
Cuts at: 0 617 881 1923
Size: 617 264 1042
Fragments arranged by size:
1042 617 264
AleI CACnn'nnGTG
Cuts at: 0 683 1923
Size: 683 1240
AlwNI CAG_nnn'CTG
Cuts at: 0 1044 1170 1923
Size: 1044 126 753
Fragments arranged by size:
1044 753 126
AvaI C'yCGr_G
Cuts at: 0 346 1306 1849 1923
Size: 346 960 543 74
Fragments arranged by size:
960 543 346 74
BanI G'GyrC_C
Cuts at: 0 34 607 1837 1923
Size: 34 573 1230 86
Fragments arranged by size:
1230 573 86 34
BanII G_rGCy'C
Cuts at: 0 500 527 947 1018 1673 1872 1923
Size: 500 27 420 71 655 199 51
Fragments arranged by size:
655 500 420 199 71 51 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1423 1457 1923
Size: 1423 34 466
Fragments arranged by size:
1423 466 34
BclI T'GATC_A
Cuts at: 0 281 428 1238 1923
Size: 281 147 810 685
Fragments arranged by size:
810 685 281 147
BglI GCCn_nnn'nGGC
Cuts at: 0 1114 1923
Size: 1114 809
BglII A'GATC_T
Cuts at: 0 353 1277 1923
Size: 353 924 646
Fragments arranged by size:
924 646 353
BlpI GC'TnA_GC
Cuts at: 0 1381 1923
Size: 1381 542
Bme1580I G_kGCm'C
Cuts at: 0 37 610 1415 1923
Size: 37 573 805 508
Fragments arranged by size:
805 573 508 37
BmgBI CAC'GTC
Cuts at: 0 618 884 1185 1923
Size: 618 266 301 738
Fragments arranged by size:
738 618 301 266
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 372 870 933 1749 1923
Size: 372 498 63 816 174
Fragments arranged by size:
816 498 372 174 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 865 961 1228 1645 1816 1923
Size: 865 96 267 417 171 107
Fragments arranged by size:
865 417 267 171 107 96
Bpu10I CC'TnA_GC
Cuts at: 0 1528 1871 1923
Size: 1528 343 52
Fragments arranged by size:
1528 343 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1657 1923
Size: 1657 266
BsaAI yAC'GTr
Cuts at: 0 123 1923
Size: 123 1800
BsaBI GATnn'nnATC
Cuts at: 0 22 1504 1923
Size: 22 1482 419
Fragments arranged by size:
1482 419 22
BsaHI Gr'CG_yC
Cuts at: 0 968 1745 1923
Size: 968 777 178
Fragments arranged by size:
968 777 178
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 343 373 1789 1819 1923
Size: 343 30 1416 30 104
Fragments arranged by size:
1416 343 104 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 364 742 922 1552 1923
Size: 364 378 180 630 371
Fragments arranged by size:
630 378 371 364 180
BseYI C'CCAG_C
Cuts at: 0 115 1200 1923
Size: 115 1085 723
Fragments arranged by size:
1085 723 115
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 328 1057 1144 1183 1576 1612 1923
Size: 268 60 729 87 39 393 36 311
Fragments arranged by size:
729 393 311 268 87 60 39 36
BsiEI CG_ry'CG
Cuts at: 0 20 225 533 635 668 1217 1923
Size: 20 205 308 102 33 549 706
Fragments arranged by size:
706 549 308 205 102 33 20
BsiHKAI G_wGCw'C
Cuts at: 0 587 919 1483 1781 1923
Size: 587 332 564 298 142
Fragments arranged by size:
587 564 332 298 142
BsmI GAATG_Cn'
Cuts at: 0 337 1923
Size: 337 1586
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1204 1923
Size: 1204 719
Bsp1286I G_dGCh'C
Cuts at: 0 37 500 527 587 610 919 947 1018
Size: 37 463 27 60 23 309 28 71
Cuts at: 1018 1415 1483 1673 1781 1872 1923
Size: 397 68 190 108 91 51
Fragments arranged by size:
463 397 309 190 108 91 71 68
60 51 37 28 27 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 300 1178 1791 1923
Size: 300 878 613 132
Fragments arranged by size:
878 613 300 132
BsrBI CCG'CTC
Cuts at: 0 144 1385 1923
Size: 144 1241 538
Fragments arranged by size:
1241 538 144
BsrFI r'CCGG_y
Cuts at: 0 438 1483 1840 1923
Size: 438 1045 357 83
Fragments arranged by size:
1045 438 357 83
BssSI C'ACGA_G
Cuts at: 0 1734 1923
Size: 1734 189
BstAPI GCAn_nnn'nTGC
Cuts at: 0 797 1044 1170 1923
Size: 797 247 126 753
Fragments arranged by size:
797 753 247 126
BstEII G'GTnAC_C
Cuts at: 0 414 1425 1923
Size: 414 1011 498
Fragments arranged by size:
1011 498 414
BstXI CCAn_nnnn'nTGG
Cuts at: 0 123 685 1140 1755 1923
Size: 123 562 455 615 168
Fragments arranged by size:
615 562 455 168 123
BstYI r'GATC_y
Cuts at: 0 353 1277 1923
Size: 353 924 646
Fragments arranged by size:
924 646 353
BtgI C'CryG_G
Cuts at: 0 11 362 527 662 947 1469 1655 1923
Size: 11 351 165 135 285 522 186 268
Fragments arranged by size:
522 351 285 268 186 165 135 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 69 525 1923
Size: 69 456 1398
Fragments arranged by size:
1398 456 69
ClaI AT'CG_AT
Cuts at: 0 1439 1923
Size: 1439 484
DraIII CAC_nnn'GTG
Cuts at: 0 723 1923
Size: 723 1200
DrdI GACnn_nn'nnGTC
Cuts at: 0 822 1923
Size: 822 1101
EaeI y'GGCC_r
Cuts at: 0 2 161 222 530 665 1130 1214 1466
Size: 2 159 61 308 135 465 84 252
Cuts at: 1466 1757 1923
Size: 291 166
Fragments arranged by size:
465 308 291 252 166 159 135 84
61 2
EagI C'GGCC_G
Cuts at: 0 222 530 665 1214 1923
Size: 222 308 135 549 709
Fragments arranged by size:
709 549 308 222 135
EarI CTCTTCn'nnn_
Cuts at: 0 1642 1923
Size: 1642 281
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1579 1923
Size: 1579 344
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 865 961 1228 1645 1816 1923
Size: 865 96 267 417 171 107
Fragments arranged by size:
865 417 267 171 107 96
EcoNI CCTnn'n_nnAGG
Cuts at: 0 801 862 1531 1923
Size: 801 61 669 392
Fragments arranged by size:
801 669 392 61
EcoO109I rG'GnC_Cy
Cuts at: 0 815 1167 1876 1900 1923
Size: 815 352 709 24 23
Fragments arranged by size:
815 709 352 24 23
FspI TGC'GCA
Cuts at: 0 790 1923
Size: 790 1133
HaeII r_GCGC'y
Cuts at: 0 181 465 1374 1655 1923
Size: 181 284 909 281 268
Fragments arranged by size:
909 284 281 268 181
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 343 375 1591 1623 1923
Size: 343 32 1216 32 300
Fragments arranged by size:
1216 343 300 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 233 944 1319 1923
Size: 87 146 711 375 604
Fragments arranged by size:
711 604 375 146 87
MscI TGG'CCA
Cuts at: 0 4 1923
Size: 4 1919
MslI CAynn'nnrTG
Cuts at: 0 683 1138 1923
Size: 683 455 785
Fragments arranged by size:
785 683 455
MspA1I CmG'CkG
Cuts at: 0 13 1823 1923
Size: 13 1810 100
Fragments arranged by size:
1810 100 13
NaeI GCC'GGC
Cuts at: 0 440 1923
Size: 440 1483
NcoI C'CATG_G
Cuts at: 0 362 1923
Size: 362 1561
NgoMIV G'CCGG_C
Cuts at: 0 438 1923
Size: 438 1485
PasI CC'CwG_GG
Cuts at: 0 1403 1923
Size: 1403 520
PflMI CCAn_nnn'nTGG
Cuts at: 0 723 1923
Size: 723 1200
PpuMI rG'GwC_Cy
Cuts at: 0 815 1167 1876 1923
Size: 815 352 709 47
Fragments arranged by size:
815 709 352 47
PshAI GACnn'nnGTC
Cuts at: 0 1718 1923
Size: 1718 205
PstI C_TGCA'G
Cuts at: 0 1052 1923
Size: 1052 871
PvuI CG_AT'CG
Cuts at: 0 20 635 1923
Size: 20 615 1288
Fragments arranged by size:
1288 615 20
SacII CC_GC'GG
Cuts at: 0 14 1923
Size: 14 1909
SanDI GG'GwC_CC
Cuts at: 0 1876 1923
Size: 1876 47
SapI GCTCTTCn'nnn_
Cuts at: 0 1642 1923
Size: 1642 281
SexAI A'CCwGG_T
Cuts at: 0 170 998 1923
Size: 170 828 925
Fragments arranged by size:
925 828 170
SfcI C'TryA_G
Cuts at: 0 1048 1923
Size: 1048 875
SmaI CCC'GGG
Cuts at: 0 1308 1851 1923
Size: 1308 543 72
Fragments arranged by size:
1308 543 72
SmlI C'TyrA_G
Cuts at: 0 1672 1923
Size: 1672 251
StyI C'CwwG_G
Cuts at: 0 302 332 362 473 1031 1754 1880 1923
Size: 302 30 30 111 558 723 126 43
Fragments arranged by size:
723 558 302 126 111 43 30 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 673 691 847 1923
Size: 673 18 156 1076
Fragments arranged by size:
1076 673 156 18
TatI w'GTAC_w
Cuts at: 0 1571 1923
Size: 1571 352
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1789 1821 1923
Size: 1789 32 102
Fragments arranged by size:
1789 102 32
Tth111I GACn'n_nGTC
Cuts at: 0 819 1000 1923
Size: 819 181 923
Fragments arranged by size:
923 819 181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 171 1620 1840 1923
Size: 171 1449 220 83
Fragments arranged by size:
1449 220 171 83
XmaI C'CCGG_G
Cuts at: 0 1306 1849 1923
Size: 1306 543 74
Fragments arranged by size:
1306 543 74
XmnI GAAnn'nnTTC
Cuts at: 0 718 1923
Size: 718 1205
Enzymes that do cut:
AarI AflIII AleI AlwNI AvaI BanI BanII BcgI
BclI BglI BglII BlpI Bme1580I BmgBI BplI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI
ClaI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV PasI PflMI PpuMI PshAI
PstI PvuI SacII SanDI SapI SexAI SfcI SmaI
SmlI StyI TaqII TatI TstI Tth111I XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BciVI
BfrBI BmrI BmtI BsaI BsaWI BsiWI BspEI BspHI
BsrDI BsrGI BssHII BstBI BstZ17I Bsu36I BtsI CspCI
DraI EcoICRI EcoRI EcoRV FalI FseI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI NarI NdeI
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuII
RsrII SacI SalI SbfI ScaI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
XbaI XhoI ZraI