Restriction map for
pF1KB9774
(Linear) (Six-base) MAPSORT of: KIBB9774.seq Check: 5355 from: 1 to: 2013
KIBB9774.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1772 1952 2013
Size: 1772 180 61
Fragments arranged by size:
1772 180 61
AccI GT'mk_AC
Cuts at: 0 602 2013
Size: 602 1411
AflIII A'CryG_T
Cuts at: 0 1889 2013
Size: 1889 124
AhdI GACnn_n'nnGTC
Cuts at: 0 978 1092 2013
Size: 978 114 921
Fragments arranged by size:
978 921 114
AlwNI CAG_nnn'CTG
Cuts at: 0 36 51 90 357 1287 1362 1496 1782
Size: 36 15 39 267 930 75 134 286
Cuts at: 1782 1848 2013
Size: 66 165
Fragments arranged by size:
930 286 267 165 134 75 66 39
36 15
ApaI G_GGCC'C
Cuts at: 0 84 1972 2013
Size: 84 1888 41
Fragments arranged by size:
1888 84 41
ApaLI G'TGCA_C
Cuts at: 0 1811 1951 2013
Size: 1811 140 62
Fragments arranged by size:
1811 140 62
ApoI r'AATT_y
Cuts at: 0 424 2013
Size: 424 1589
AvaI C'yCGr_G
Cuts at: 0 558 1314 1326 1366 1561 1674 1965 2013
Size: 558 756 12 40 195 113 291 48
Fragments arranged by size:
756 558 291 195 113 48 40 12
BamHI G'GATC_C
Cuts at: 0 819 1053 2013
Size: 819 234 960
Fragments arranged by size:
960 819 234
BanI G'GyrC_C
Cuts at: 0 231 846 1389 1534 1975 2013
Size: 231 615 543 145 441 38
Fragments arranged by size:
615 543 441 231 145 38
BanII G_rGCy'C
Cuts at: 0 84 266 724 761 842 1972 2013
Size: 84 182 458 37 81 1130 41
Fragments arranged by size:
1130 458 182 84 81 41 37
BbeI G_GCGC'C
Cuts at: 0 1393 1538 1979 2013
Size: 1393 145 441 34
Fragments arranged by size:
1393 441 145 34
BbsI GAAGACnn'nnnn_
Cuts at: 0 231 632 1747 2013
Size: 231 401 1115 266
Fragments arranged by size:
1115 401 266 231
BbvCI CC'TCA_GC
Cuts at: 0 29 2013
Size: 29 1984
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1560 1594 2013
Size: 1560 34 419
Fragments arranged by size:
1560 419 34
BglI GCCn_nnn'nGGC
Cuts at: 0 316 1676 2013
Size: 316 1360 337
Fragments arranged by size:
1360 337 316
BglII A'GATC_T
Cuts at: 0 284 2013
Size: 284 1729
BlpI GC'TnA_GC
Cuts at: 0 755 2013
Size: 755 1258
Bme1580I G_kGCm'C
Cuts at: 0 84 234 1561 1815 1955 1972 2013
Size: 84 150 1327 254 140 17 41
Fragments arranged by size:
1327 254 150 140 84 41 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 261 2013
Size: 261 1752
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1395 2013
Size: 1395 618
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 385 523 728 937 2013
Size: 93 292 138 205 209 1076
Fragments arranged by size:
1076 292 209 205 138 93
Bpu10I CC'TnA_GC
Cuts at: 0 29 2003 2013
Size: 29 1974 10
Fragments arranged by size:
1974 29 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 1837 1918 2013
Size: 152 1685 81 95
Fragments arranged by size:
1685 152 95 81
BsaAI yAC'GTr
Cuts at: 0 1892 2013
Size: 1892 121
BsaHI Gr'CG_yC
Cuts at: 0 185 1390 1535 1976 2013
Size: 185 1205 145 441 37
Fragments arranged by size:
1205 441 185 145 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 433 463 991 1021 1973 2003 2013
Size: 433 30 528 30 952 30 10
Fragments arranged by size:
952 528 433 30 30 30 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 1012 1126 1513 2013
Size: 115 897 114 387 500
Fragments arranged by size:
897 500 387 115 114
BseYI C'CCAG_C
Cuts at: 0 36 77 459 1229 1235 1241 1274 2013
Size: 36 41 382 770 6 6 33 739
Fragments arranged by size:
770 739 382 41 36 33 6 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1513 1768 2013
Size: 1513 255 245
Fragments arranged by size:
1513 255 245
BsiEI CG_ry'CG
Cuts at: 0 22 249 419 2013
Size: 22 227 170 1594
Fragments arranged by size:
1594 227 170 22
BsiHKAI G_wGCw'C
Cuts at: 0 1729 1763 1815 1955 2013
Size: 1729 34 52 140 58
Fragments arranged by size:
1729 140 58 52 34
BsmI GAATG_Cn'
Cuts at: 0 155 2013
Size: 155 1858
Bsp1286I G_dGCh'C
Cuts at: 0 84 234 266 724 761 842 1561 1729
Size: 84 150 32 458 37 81 719 168
Cuts at: 1729 1763 1815 1955 1972 2013
Size: 34 52 140 17 41
Fragments arranged by size:
719 458 168 150 140 84 81 52
41 37 34 32 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1772 1856 1952 2013
Size: 1772 84 96 61
Fragments arranged by size:
1772 96 84 61
BsrBI CCG'CTC
Cuts at: 0 1629 1713 1797 2013
Size: 1629 84 84 216
Fragments arranged by size:
1629 216 84 84
BsrDI GCAATG_nn'
Cuts at: 0 605 2013
Size: 605 1408
BsrFI r'CCGG_y
Cuts at: 0 120 2013
Size: 120 1893
BssSI C'ACGA_G
Cuts at: 0 1554 2013
Size: 1554 459
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1496 1848 2013
Size: 1496 352 165
Fragments arranged by size:
1496 352 165
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1127 1302 2013
Size: 1127 175 711
Fragments arranged by size:
1127 711 175
BstYI r'GATC_y
Cuts at: 0 284 819 1053 2013
Size: 284 535 234 960
Fragments arranged by size:
960 535 284 234
BtgI C'CryG_G
Cuts at: 0 419 1049 1126 1704 1981 2013
Size: 419 630 77 578 277 32
Fragments arranged by size:
630 578 419 277 77 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1281 1445 2013
Size: 1281 164 568
Fragments arranged by size:
1281 568 164
DraIII CAC_nnn'GTG
Cuts at: 0 893 2013
Size: 893 1120
EaeI y'GGCC_r
Cuts at: 0 19 140 193 398 416 1304 2013
Size: 19 121 53 205 18 888 709
Fragments arranged by size:
888 709 205 121 53 19 18
EagI C'GGCC_G
Cuts at: 0 19 416 2013
Size: 19 397 1597
Fragments arranged by size:
1597 397 19
EarI CTCTTCn'nnn_
Cuts at: 0 794 1651 2013
Size: 794 857 362
Fragments arranged by size:
857 794 362
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1530 1675 2013
Size: 1530 145 338
Fragments arranged by size:
1530 338 145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 385 523 728 937 2013
Size: 93 292 138 205 209 1076
Fragments arranged by size:
1076 292 209 205 138 93
EcoNI CCTnn'n_nnAGG
Cuts at: 0 472 679 2013
Size: 472 207 1334
Fragments arranged by size:
1334 472 207
EcoO109I rG'GnC_Cy
Cuts at: 0 48 464 543 576 912 972 1969 2013
Size: 48 416 79 33 336 60 997 44
Fragments arranged by size:
997 416 336 79 60 48 44 33
FspI TGC'GCA
Cuts at: 0 526 854 1588 2013
Size: 526 328 734 425
Fragments arranged by size:
734 526 425 328
HaeII r_GCGC'y
Cuts at: 0 127 792 1393 1538 1884 1979 2013
Size: 127 665 601 145 346 95 34
Fragments arranged by size:
665 601 346 145 127 95 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 850 882 964 996 1078 1110 2013
Size: 850 32 82 32 82 32 903
Fragments arranged by size:
903 850 82 82 32 32 32
HincII GTy'rAC
Cuts at: 0 14 603 2013
Size: 14 589 1410
Fragments arranged by size:
1410 589 14
KasI G'GCGC_C
Cuts at: 0 1389 1534 1975 2013
Size: 1389 145 441 38
Fragments arranged by size:
1389 441 145 38
MslI CAynn'nnrTG
Cuts at: 0 610 1125 1300 2013
Size: 610 515 175 713
Fragments arranged by size:
713 610 515 175
MspA1I CmG'CkG
Cuts at: 0 36 65 354 909 1260 1493 1706 1779
Size: 36 29 289 555 351 233 213 73
Cuts at: 1779 1958 2013
Size: 179 55
Fragments arranged by size:
555 351 289 233 213 179 73 55
36 29
NaeI GCC'GGC
Cuts at: 0 122 2013
Size: 122 1891
NarI GG'CG_CC
Cuts at: 0 1390 1535 1976 2013
Size: 1390 145 441 37
Fragments arranged by size:
1390 441 145 37
NcoI C'CATG_G
Cuts at: 0 1126 2013
Size: 1126 887
NgoMIV G'CCGG_C
Cuts at: 0 120 2013
Size: 120 1893
PasI CC'CwG_GG
Cuts at: 0 761 2013
Size: 761 1252
PflMI CCAn_nnn'nTGG
Cuts at: 0 51 2013
Size: 51 1962
PfoI T'CCnGG_A
Cuts at: 0 982 2013
Size: 982 1031
PmlI CAC'GTG
Cuts at: 0 1892 2013
Size: 1892 121
PpuMI rG'GwC_Cy
Cuts at: 0 464 543 972 2013
Size: 464 79 429 1041
Fragments arranged by size:
1041 464 429 79
PshAI GACnn'nnGTC
Cuts at: 0 598 2013
Size: 598 1415
PsiI TTA'TAA
Cuts at: 0 668 2013
Size: 668 1345
PspOMI G'GGCC_C
Cuts at: 0 80 1968 2013
Size: 80 1888 45
Fragments arranged by size:
1888 80 45
PstI C_TGCA'G
Cuts at: 0 476 494 1265 1769 2013
Size: 476 18 771 504 244
Fragments arranged by size:
771 504 476 244 18
PvuII CAG'CTG
Cuts at: 0 36 354 1260 1493 2013
Size: 36 318 906 233 520
Fragments arranged by size:
906 520 318 233 36
SacII CC_GC'GG
Cuts at: 0 1707 2013
Size: 1707 306
SalI G'TCGA_C
Cuts at: 0 601 2013
Size: 601 1412
SanDI GG'GwC_CC
Cuts at: 0 464 2013
Size: 464 1549
SapI GCTCTTCn'nnn_
Cuts at: 0 1651 2013
Size: 1651 362
SfcI C'TryA_G
Cuts at: 0 472 490 1261 1746 1765 2013
Size: 472 18 771 485 19 248
Fragments arranged by size:
771 485 472 248 19 18
SfoI GGC'GCC
Cuts at: 0 1391 1536 1977 2013
Size: 1391 145 441 36
Fragments arranged by size:
1391 441 145 36
SmaI CCC'GGG
Cuts at: 0 560 1316 1368 1967 2013
Size: 560 756 52 599 46
Fragments arranged by size:
756 599 560 52 46
SmlI C'TyrA_G
Cuts at: 0 167 1852 1933 2013
Size: 167 1685 81 80
Fragments arranged by size:
1685 167 81 80
SrfI GCCC'GGGC
Cuts at: 0 1967 2013
Size: 1967 46
StyI C'CwwG_G
Cuts at: 0 581 1126 1622 2013
Size: 581 545 496 391
Fragments arranged by size:
581 545 496 391
TatI w'GTAC_w
Cuts at: 0 614 2013
Size: 614 1399
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 224 256 433 465 877 909 991 1023
Size: 224 32 177 32 412 32 82 32
Cuts at: 1023 1973 2005 2013
Size: 950 32 8
Fragments arranged by size:
950 412 224 177 82 32 32 32
32 32 8
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 318 2013
Size: 318 1695
XmaI C'CCGG_G
Cuts at: 0 558 1314 1366 1965 2013
Size: 558 756 52 599 48
Fragments arranged by size:
756 599 558 52 48
XmnI GAAnn'nnTTC
Cuts at: 0 1660 2013
Size: 1660 353
Enzymes that do cut:
AarI AccI AflIII AhdI AlwNI ApaI ApaLI ApoI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstXI BstYI BtgI BtgZI DraIII EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I
HincII KasI MslI MspA1I NaeI NarI NcoI NgoMIV
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PstI PvuII SacII SalI SanDI SapI SfcI SfoI
SmaI SmlI SrfI StyI TatI TstI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AleI
AlfI AloI AscI AseI AsiSI AvrII BaeI BciVI
BclI BfrBI BmgBI BmtI BsaI BsaBI BsaWI BsiWI
BsmBI BspEI BspHI BsrGI BssHII BstBI BstEII BstZ17I
Bsu36I BtsI ClaI CspCI DraI DrdI EcoICRI EcoRI
EcoRV FalI FseI FspAI HindIII HpaI KpnI MfeI
MluI MmeI MscI NdeI NheI NotI NruI NsiI
NspI PacI PciI PmeI PpiI PspXI PsrI PvuI
RsrII SacI SbfI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SphI SspI StuI SwaI TaqII Tth111I
XbaI XhoI ZraI