Restriction map for
pF1KB9777
(Linear) (Six-base) MAPSORT of: KIBB9777.seq Check: 5529 from: 1 to: 2364
KIBB9777.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AccI GT'mk_AC
Cuts at: 0 2087 2364
Size: 2087 277
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 476 871 928 1501 2364
Size: 7 469 395 57 573 863
Fragments arranged by size:
863 573 469 395 57 7
AleI CACnn'nnGTG
Cuts at: 0 569 1278 2364
Size: 569 709 1086
Fragments arranged by size:
1086 709 569
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1723 1755 2364
Size: 1723 32 609
Fragments arranged by size:
1723 609 32
AlwNI CAG_nnn'CTG
Cuts at: 0 837 1326 2364
Size: 837 489 1038
Fragments arranged by size:
1038 837 489
ApaI G_GGCC'C
Cuts at: 0 1301 2364
Size: 1301 1063
ApoI r'AATT_y
Cuts at: 0 258 306 1123 2364
Size: 258 48 817 1241
Fragments arranged by size:
1241 817 258 48
AseI AT'TA_AT
Cuts at: 0 266 2364
Size: 266 2098
AvaI C'yCGr_G
Cuts at: 0 94 805 1304 1973 2364
Size: 94 711 499 669 391
Fragments arranged by size:
711 669 499 391 94
BamHI G'GATC_C
Cuts at: 0 2002 2364
Size: 2002 362
BanI G'GyrC_C
Cuts at: 0 1029 2364
Size: 1029 1335
BanII G_rGCy'C
Cuts at: 0 302 929 1301 2364
Size: 302 627 372 1063
Fragments arranged by size:
1063 627 372 302
BbeI G_GCGC'C
Cuts at: 0 1033 2364
Size: 1033 1331
BbsI GAAGACnn'nnnn_
Cuts at: 0 308 877 1276 1295 1489 2364
Size: 308 569 399 19 194 875
Fragments arranged by size:
875 569 399 308 194 19
BclI T'GATC_A
Cuts at: 0 828 2364
Size: 828 1536
BlpI GC'TnA_GC
Cuts at: 0 991 2364
Size: 991 1373
Bme1580I G_kGCm'C
Cuts at: 0 1301 2364
Size: 1301 1063
BmrI ACTGGGnnnn_n'
Cuts at: 0 741 1314 1529 2364
Size: 741 573 215 835
Fragments arranged by size:
835 741 573 215
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 709 877 1348 1562 1741 2364
Size: 227 482 168 471 214 179 623
Fragments arranged by size:
623 482 471 227 214 179 168
Bpu10I CC'TnA_GC
Cuts at: 0 480 2364
Size: 480 1884
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 2364
Size: 286 2078
BsaI GGTCTCn'nnnn_
Cuts at: 0 949 2024 2364
Size: 949 1075 340
Fragments arranged by size:
1075 949 340
BsaHI Gr'CG_yC
Cuts at: 0 1030 2364
Size: 1030 1334
BsaWI w'CCGG_w
Cuts at: 0 737 2320 2364
Size: 737 1583 44
Fragments arranged by size:
1583 737 44
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 200 230 1555 1585 1723 1753 2364
Size: 200 30 1325 30 138 30 611
Fragments arranged by size:
1325 611 200 138 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 430 1007 1828 2254 2364
Size: 430 577 821 426 110
Fragments arranged by size:
821 577 430 426 110
BseYI C'CCAG_C
Cuts at: 0 939 1033 1734 2364
Size: 939 94 701 630
Fragments arranged by size:
939 701 630 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 2364
Size: 799 1565
BsiHKAI G_wGCw'C
Cuts at: 0 302 929 1285 1576 1622 2364
Size: 302 627 356 291 46 742
Fragments arranged by size:
742 627 356 302 291 46
BsmI GAATG_Cn'
Cuts at: 0 950 1607 2364
Size: 950 657 757
Fragments arranged by size:
950 757 657
Bsp1286I G_dGCh'C
Cuts at: 0 302 929 1285 1301 1576 1622 2364
Size: 302 627 356 16 275 46 742
Fragments arranged by size:
742 627 356 302 275 46 16
BspEI T'CCGG_A
Cuts at: 0 2320 2364
Size: 2320 44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 639 1332 2364
Size: 639 693 1032
Fragments arranged by size:
1032 693 639
BsrDI GCAATG_nn'
Cuts at: 0 300 1836 2364
Size: 300 1536 528
Fragments arranged by size:
1536 528 300
BsrFI r'CCGG_y
Cuts at: 0 627 773 2364
Size: 627 146 1591
Fragments arranged by size:
1591 627 146
BssSI C'ACGA_G
Cuts at: 0 1615 2364
Size: 1615 749
BstEII G'GTnAC_C
Cuts at: 0 574 2364
Size: 574 1790
BstXI CCAn_nnnn'nTGG
Cuts at: 0 855 2364
Size: 855 1509
BstYI r'GATC_y
Cuts at: 0 1188 1476 2002 2317 2364
Size: 1188 288 526 315 47
Fragments arranged by size:
1188 526 315 288 47
Bsu36I CC'TnA_GG
Cuts at: 0 107 404 1430 2364
Size: 107 297 1026 934
Fragments arranged by size:
1026 934 297 107
BtgI C'CryG_G
Cuts at: 0 1025 2364
Size: 1025 1339
BtsI GCAGTG_nn'
Cuts at: 0 576 609 1907 2364
Size: 576 33 1298 457
Fragments arranged by size:
1298 576 457 33
DraI TTT'AAA
Cuts at: 0 2160 2364
Size: 2160 204
DrdI GACnn_nn'nnGTC
Cuts at: 0 738 2364
Size: 738 1626
EaeI y'GGCC_r
Cuts at: 0 1689 2064 2364
Size: 1689 375 300
Fragments arranged by size:
1689 375 300
EarI CTCTTCn'nnn_
Cuts at: 0 25 371 662 904 1439 2231 2364
Size: 25 346 291 242 535 792 133
Fragments arranged by size:
792 535 346 291 242 133 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1869 2364
Size: 1869 495
EcoICRI GAG'CTC
Cuts at: 0 300 927 2364
Size: 300 627 1437
Fragments arranged by size:
1437 627 300
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 227 476 709 871 877 928 1348
Size: 7 220 249 233 162 6 51 420
Cuts at: 1348 1501 1562 1741 2364
Size: 153 61 179 623
Fragments arranged by size:
623 420 249 233 220 179 162 153
61 51 7 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 332 2364
Size: 332 2032
EcoO109I rG'GnC_Cy
Cuts at: 0 80 408 789 1297 2364
Size: 80 328 381 508 1067
Fragments arranged by size:
1067 508 381 328 80
EcoRI G'AATT_C
Cuts at: 0 306 2364
Size: 306 2058
EcoRV GAT'ATC
Cuts at: 0 1866 2364
Size: 1866 498
HaeII r_GCGC'y
Cuts at: 0 528 1033 1720 2364
Size: 528 505 687 644
Fragments arranged by size:
687 644 528 505
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1560 1592 2251 2283 2302 2334 2364
Size: 1560 32 659 32 19 32 30
Fragments arranged by size:
1560 659 32 32 32 30 19
HincII GTy'rAC
Cuts at: 0 2088 2364
Size: 2088 276
KasI G'GCGC_C
Cuts at: 0 1029 2364
Size: 1029 1335
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 331 1454 2364
Size: 112 219 1123 910
Fragments arranged by size:
1123 910 219 112
MscI TGG'CCA
Cuts at: 0 1691 2066 2364
Size: 1691 375 298
Fragments arranged by size:
1691 375 298
MslI CAynn'nnrTG
Cuts at: 0 155 569 1278 2364
Size: 155 414 709 1086
Fragments arranged by size:
1086 709 414 155
MspA1I CmG'CkG
Cuts at: 0 855 939 1071 2019 2364
Size: 855 84 132 948 345
Fragments arranged by size:
948 855 345 132 84
NaeI GCC'GGC
Cuts at: 0 629 775 2364
Size: 629 146 1589
Fragments arranged by size:
1589 629 146
NarI GG'CG_CC
Cuts at: 0 1030 2364
Size: 1030 1334
NgoMIV G'CCGG_C
Cuts at: 0 627 773 2364
Size: 627 146 1591
Fragments arranged by size:
1591 627 146
PasI CC'CwG_GG
Cuts at: 0 247 2364
Size: 247 2117
PflMI CCAn_nnn'nTGG
Cuts at: 0 1326 1634 2364
Size: 1326 308 730
Fragments arranged by size:
1326 730 308
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1723 1755 2364
Size: 1723 32 609
Fragments arranged by size:
1723 609 32
PpuMI rG'GwC_Cy
Cuts at: 0 80 408 789 2364
Size: 80 328 381 1575
Fragments arranged by size:
1575 381 328 80
PspOMI G'GGCC_C
Cuts at: 0 1297 2364
Size: 1297 1067
PspXI vC'TCGA_Gb
Cuts at: 0 805 2364
Size: 805 1559
PstI C_TGCA'G
Cuts at: 0 168 650 1343 1712 2364
Size: 168 482 693 369 652
Fragments arranged by size:
693 652 482 369 168
PvuII CAG'CTG
Cuts at: 0 855 939 1071 2364
Size: 855 84 132 1293
Fragments arranged by size:
1293 855 132 84
SacI G_AGCT'C
Cuts at: 0 302 929 2364
Size: 302 627 1435
Fragments arranged by size:
1435 627 302
SalI G'TCGA_C
Cuts at: 0 2086 2364
Size: 2086 278
SanDI GG'GwC_CC
Cuts at: 0 789 2364
Size: 789 1575
SapI GCTCTTCn'nnn_
Cuts at: 0 662 904 2364
Size: 662 242 1460
Fragments arranged by size:
1460 662 242
SfcI C'TryA_G
Cuts at: 0 164 646 1099 1339 1708 2364
Size: 164 482 453 240 369 656
Fragments arranged by size:
656 482 453 369 240 164
SfoI GGC'GCC
Cuts at: 0 1031 2364
Size: 1031 1333
SmaI CCC'GGG
Cuts at: 0 96 1306 2364
Size: 96 1210 1058
Fragments arranged by size:
1210 1058 96
SmlI C'TyrA_G
Cuts at: 0 301 805 1973 2364
Size: 301 504 1168 391
Fragments arranged by size:
1168 504 391 301
SspI AAT'ATT
Cuts at: 0 43 2364
Size: 43 2321
StuI AGG'CCT
Cuts at: 0 1883 2364
Size: 1883 481
StyI C'CwwG_G
Cuts at: 0 968 1460 1628 2364
Size: 968 492 168 736
Fragments arranged by size:
968 736 492 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 167 2364
Size: 167 2197
TatI w'GTAC_w
Cuts at: 0 426 1549 2078 2228 2364
Size: 426 1123 529 150 136
Fragments arranged by size:
1123 529 426 150 136
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 200 232 1555 1587 1629 1661 1994 2026
Size: 200 32 1323 32 42 32 333 32
Cuts at: 2026 2364
Size: 338
Fragments arranged by size:
1323 338 333 200 42 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 61 976 2364
Size: 61 915 1388
Fragments arranged by size:
1388 915 61
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1068 2364
Size: 1068 1296
XhoI C'TCGA_G
Cuts at: 0 805 1973 2364
Size: 805 1168 391
Fragments arranged by size:
1168 805 391
XmaI C'CCGG_G
Cuts at: 0 94 1304 2364
Size: 94 1210 1060
Fragments arranged by size:
1210 1060 94
Enzymes that do cut:
AccI AcuI AleI AloI AlwNI ApaI ApoI AseI
AvaI BamHI BanI BanII BbeI BbsI BclI BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrDI BsrFI BssSI BstEII BstXI BstYI Bsu36I
BtgI BtsI DraI DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
PasI PflMI PpiI PpuMI PspOMI PspXI PstI PvuII
SacI SalI SanDI SapI SfcI SfoI SmaI SmlI
SspI StuI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI ApaLI AscI AsiSI AvrII BaeI BbvCI
BcgI BciVI BfrBI BglI BglII BmgBI BmtI BplI
BsaAI BsaBI BsiEI BsiWI BsmBI BspHI BsrBI BsrGI
BssHII BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraIII
EagI FalI FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PciI PfoI PmeI PmlI PshAI PsiI
PsrI PvuI RsrII SacII SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SwaI XbaI
XmnI ZraI