Restriction map for
pF1KB9778
(Linear) (Six-base) MAPSORT of: KIBB9778.seq Check: 3850 from: 1 to: 2394
KIBB9778.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1457 2394
Size: 1457 937
AccI GT'mk_AC
Cuts at: 0 114 1491 2394
Size: 114 1377 903
Fragments arranged by size:
1377 903 114
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 522 1034 1357 2394
Size: 434 88 512 323 1037
Fragments arranged by size:
1037 512 434 323 88
AflIII A'CryG_T
Cuts at: 0 1885 2394
Size: 1885 509
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1253 1285 2394
Size: 1253 32 1109
Fragments arranged by size:
1253 1109 32
AlwNI CAG_nnn'CTG
Cuts at: 0 975 1012 2394
Size: 975 37 1382
Fragments arranged by size:
1382 975 37
ApaLI G'TGCA_C
Cuts at: 0 141 2394
Size: 141 2253
ApoI r'AATT_y
Cuts at: 0 3 147 400 729 1018 1637 1651 2293
Size: 3 144 253 329 289 619 14 642
Cuts at: 2293 2394
Size: 101
Fragments arranged by size:
642 619 329 289 253 144 101 14
3
BbsI GAAGACnn'nnnn_
Cuts at: 0 422 1535 2394
Size: 422 1113 859
Fragments arranged by size:
1113 859 422
BciVI GTATCCnnnnn_n'
Cuts at: 0 1776 2394
Size: 1776 618
BclI T'GATC_A
Cuts at: 0 32 1109 2394
Size: 32 1077 1285
Fragments arranged by size:
1285 1077 32
BfrBI ATG'CAT
Cuts at: 0 1120 2394
Size: 1120 1274
BglII A'GATC_T
Cuts at: 0 501 1814 2394
Size: 501 1313 580
Fragments arranged by size:
1313 580 501
BlpI GC'TnA_GC
Cuts at: 0 602 1922 2394
Size: 602 1320 472
Fragments arranged by size:
1320 602 472
Bme1580I G_kGCm'C
Cuts at: 0 145 2394
Size: 145 2249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 725 819 875 2394
Size: 212 513 94 56 1519
Fragments arranged by size:
1519 513 212 94 56
Bpu10I CC'TnA_GC
Cuts at: 0 607 2394
Size: 607 1787
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 2394
Size: 301 2093
BsaAI yAC'GTr
Cuts at: 0 2111 2394
Size: 2111 283
BsaBI GATnn'nnATC
Cuts at: 0 369 2394
Size: 369 2025
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1253 1283 1577 1607 2394
Size: 1253 30 294 30 787
Fragments arranged by size:
1253 787 294 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 534 2394
Size: 534 1860
BseYI C'CCAG_C
Cuts at: 0 2039 2394
Size: 2039 355
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 621 1485 1965 2394
Size: 167 454 864 480 429
Fragments arranged by size:
864 480 454 429 167
BsiHKAI G_wGCw'C
Cuts at: 0 145 668 2394
Size: 145 523 1726
Fragments arranged by size:
1726 523 145
BsmI GAATG_Cn'
Cuts at: 0 176 1399 1507 2319 2394
Size: 176 1223 108 812 75
Fragments arranged by size:
1223 812 176 108 75
Bsp1286I G_dGCh'C
Cuts at: 0 145 668 2394
Size: 145 523 1726
Fragments arranged by size:
1726 523 145
BspHI T'CATG_A
Cuts at: 0 1431 2271 2394
Size: 1431 840 123
Fragments arranged by size:
1431 840 123
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1457 1912 2394
Size: 1457 455 482
Fragments arranged by size:
1457 482 455
BsrDI GCAATG_nn'
Cuts at: 0 1101 1479 1827 2394
Size: 1101 378 348 567
Fragments arranged by size:
1101 567 378 348
BsrFI r'CCGG_y
Cuts at: 0 2043 2394
Size: 2043 351
BsrGI T'GTAC_A
Cuts at: 0 920 1858 2394
Size: 920 938 536
Fragments arranged by size:
938 920 536
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1655 2394
Size: 1655 739
BstXI CCAn_nnnn'nTGG
Cuts at: 0 20 2394
Size: 20 2374
BstYI r'GATC_y
Cuts at: 0 338 501 1684 1814 1863 2394
Size: 338 163 1183 130 49 531
Fragments arranged by size:
1183 531 338 163 130 49
BstZ17I GTA'TAC
Cuts at: 0 115 2394
Size: 115 2279
BtsI GCAGTG_nn'
Cuts at: 0 1767 2394
Size: 1767 627
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 84 119 335 370 2394
Size: 84 35 216 35 2024
Fragments arranged by size:
2024 216 84 35 35
DraI TTT'AAA
Cuts at: 0 83 1329 2394
Size: 83 1246 1065
Fragments arranged by size:
1246 1065 83
DraIII CAC_nnn'GTG
Cuts at: 0 190 2394
Size: 190 2204
EaeI y'GGCC_r
Cuts at: 0 1824 2394
Size: 1824 570
EarI CTCTTCn'nnn_
Cuts at: 0 1612 2394
Size: 1612 782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 434 522 725 819 875 1034 1357
Size: 212 222 88 203 94 56 159 323
Cuts at: 1357 2394
Size: 1037
Fragments arranged by size:
1037 323 222 212 203 159 94 88
56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1577 1609 2047 2079 2394
Size: 1577 32 438 32 315
Fragments arranged by size:
1577 438 315 32 32
HincII GTy'rAC
Cuts at: 0 1521 2088 2394
Size: 1521 567 306
Fragments arranged by size:
1521 567 306
HindIII A'AGCT_T
Cuts at: 0 2254 2394
Size: 2254 140
HpaI GTT'AAC
Cuts at: 0 1521 2394
Size: 1521 873
MfeI C'AATT_G
Cuts at: 0 2192 2394
Size: 2192 202
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 687 2073 2394
Size: 366 321 1386 321
Fragments arranged by size:
1386 366 321 321
MslI CAynn'nnrTG
Cuts at: 0 938 1452 1989 2394
Size: 938 514 537 405
Fragments arranged by size:
938 537 514 405
NsiI A_TGCA'T
Cuts at: 0 1122 2394
Size: 1122 1272
NspI r_CATG'y
Cuts at: 0 1769 1982 2394
Size: 1769 213 412
Fragments arranged by size:
1769 412 213
PflMI CCAn_nnn'nTGG
Cuts at: 0 133 748 892 2394
Size: 133 615 144 1502
Fragments arranged by size:
1502 615 144 133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1253 1285 2394
Size: 1253 32 1109
Fragments arranged by size:
1253 1109 32
PsiI TTA'TAA
Cuts at: 0 2348 2394
Size: 2348 46
PstI C_TGCA'G
Cuts at: 0 1549 1576 2394
Size: 1549 27 818
Fragments arranged by size:
1549 818 27
ScaI AGT'ACT
Cuts at: 0 1791 2394
Size: 1791 603
SfcI C'TryA_G
Cuts at: 0 1545 1572 2394
Size: 1545 27 822
Fragments arranged by size:
1545 822 27
SmlI C'TyrA_G
Cuts at: 0 316 2394
Size: 316 2078
SnaBI TAC'GTA
Cuts at: 0 2111 2394
Size: 2111 283
SpeI A'CTAG_T
Cuts at: 0 1786 2394
Size: 1786 608
SspI AAT'ATT
Cuts at: 0 990 1251 2394
Size: 990 261 1143
Fragments arranged by size:
1143 990 261
StuI AGG'CCT
Cuts at: 0 1338 2394
Size: 1338 1056
StyI C'CwwG_G
Cuts at: 0 1908 2012 2394
Size: 1908 104 382
Fragments arranged by size:
1908 382 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 125 2394
Size: 125 2269
TatI w'GTAC_w
Cuts at: 0 920 1376 1789 1858 2394
Size: 920 456 413 69 536
Fragments arranged by size:
920 536 456 413 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 855 887 2394
Size: 855 32 1507
Fragments arranged by size:
1507 855 32
Tth111I GACn'n_nGTC
Cuts at: 0 899 2394
Size: 899 1495
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 134 2394
Size: 134 2260
XmnI GAAnn'nnTTC
Cuts at: 0 1019 1242 1937 2394
Size: 1019 223 695 457
Fragments arranged by size:
1019 695 457 223
Enzymes that do cut:
AarI AccI AcuI AflIII AloI AlwNI ApaLI ApoI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BpmI
Bpu10I BpuEI BsaAI BsaBI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI
BstAPI BstXI BstYI BstZ17I BtsI CspCI DraI DraIII
EaeI EarI Eco57MI Hin4I HincII HindIII HpaI MfeI
MmeI MslI NsiI NspI PflMI PpiI PsiI PstI
ScaI SfcI SmlI SnaBI SpeI SspI StuI StyI
TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbvCI BcgI BglI BmgBI
BmrI BmtI BplI BsaI BsaHI BsaWI BsiEI BsiWI
BsmBI BspEI BsrBI BssHII BssSI BstBI BstEII Bsu36I
BtgI BtgZI ClaI DrdI EagI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
KasI KpnI MluI MscI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PfoI PmeI PmlI PpuMI PshAI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SphI
SrfI SwaI XbaI XhoI XmaI ZraI