(Linear) (Six-base) MAPSORT of: KIBB9780.seq Check: 740 from: 1 to: 204 KIBB9780.orf With 182 enzymes: SgfI * March 3, 2008 11:11 .. BsaI GGTCTCn'nnnn_ Cuts at: 0 87 204 Size: 87 117 BsaHI Gr'CG_yC Cuts at: 0 94 204 Size: 94 110 BspHI T'CATG_A Cuts at: 0 104 204 Size: 104 100 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 129 204 Size: 129 75 NspI r_CATG'y Cuts at: 0 127 204 Size: 127 77 Enzymes that do cut: BsaI BsaHI BspHI MmeI NspI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI