Restriction map for
pF1KB9787
(Linear) (Six-base) MAPSORT of: KIBB9787.seq Check: 8191 from: 1 to: 573
KIBB9787.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 471 573
Size: 471 102
AatII G_ACGT'C
Cuts at: 0 134 573
Size: 134 439
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 573
Size: 177 396
AleI CACnn'nnGTG
Cuts at: 0 124 573
Size: 124 449
ApaI G_GGCC'C
Cuts at: 0 374 573
Size: 374 199
BanII G_rGCy'C
Cuts at: 0 374 573
Size: 374 199
BbsI GAAGACnn'nnnn_
Cuts at: 0 126 573
Size: 126 447
BglII A'GATC_T
Cuts at: 0 566 573
Size: 566 7
BlpI GC'TnA_GC
Cuts at: 0 453 573
Size: 453 120
Bme1580I G_kGCm'C
Cuts at: 0 374 573
Size: 374 199
BmgBI CAC'GTC
Cuts at: 0 231 573
Size: 231 342
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 573
Size: 466 107
Bpu10I CC'TnA_GC
Cuts at: 0 460 573
Size: 460 113
BsaI GGTCTCn'nnnn_
Cuts at: 0 73 573
Size: 73 500
BsaHI Gr'CG_yC
Cuts at: 0 131 188 573
Size: 131 57 385
Fragments arranged by size:
385 131 57
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 367 397 573
Size: 367 30 176
Fragments arranged by size:
367 176 30
BsiHKAI G_wGCw'C
Cuts at: 0 454 573
Size: 454 119
BsmBI CGTCTCn'nnnn_
Cuts at: 0 519 573
Size: 519 54
Bsp1286I G_dGCh'C
Cuts at: 0 374 454 573
Size: 374 80 119
Fragments arranged by size:
374 119 80
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 471 573
Size: 471 102
BsrDI GCAATG_nn'
Cuts at: 0 189 573
Size: 189 384
BsrFI r'CCGG_y
Cuts at: 0 178 573
Size: 178 395
BstYI r'GATC_y
Cuts at: 0 566 573
Size: 566 7
BtgI C'CryG_G
Cuts at: 0 398 573
Size: 398 175
EaeI y'GGCC_r
Cuts at: 0 254 341 573
Size: 254 87 232
Fragments arranged by size:
254 232 87
EarI CTCTTCn'nnn_
Cuts at: 0 550 573
Size: 550 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 466 573
Size: 177 289 107
Fragments arranged by size:
289 177 107
EcoO109I rG'GnC_Cy
Cuts at: 0 370 371 380 573
Size: 370 1 9 193
Fragments arranged by size:
370 193 9 1
HincII GTy'rAC
Cuts at: 0 513 573
Size: 513 60
MscI TGG'CCA
Cuts at: 0 256 343 573
Size: 256 87 230
Fragments arranged by size:
256 230 87
MslI CAynn'nnrTG
Cuts at: 0 124 573
Size: 124 449
MspA1I CmG'CkG
Cuts at: 0 360 573
Size: 360 213
NaeI GCC'GGC
Cuts at: 0 180 573
Size: 180 393
NcoI C'CATG_G
Cuts at: 0 398 573
Size: 398 175
NgoMIV G'CCGG_C
Cuts at: 0 178 573
Size: 178 395
PfoI T'CCnGG_A
Cuts at: 0 86 573
Size: 86 487
PspOMI G'GGCC_C
Cuts at: 0 370 573
Size: 370 203
SapI GCTCTTCn'nnn_
Cuts at: 0 550 573
Size: 550 23
StuI AGG'CCT
Cuts at: 0 94 573
Size: 94 479
StyI C'CwwG_G
Cuts at: 0 398 434 573
Size: 398 36 139
Fragments arranged by size:
398 139 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 361 573
Size: 361 212
TatI w'GTAC_w
Cuts at: 0 474 573
Size: 474 99
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 367 399 573
Size: 367 32 174
Fragments arranged by size:
367 174 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 441 573
Size: 441 132
ZraI GAC'GTC
Cuts at: 0 132 573
Size: 132 441
Enzymes that do cut:
AarI AatII AcuI AleI ApaI BanII BbsI BglII
BlpI Bme1580I BmgBI BpmI Bpu10I BsaI BsaHI BsaXI
BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BstYI BtgI
EaeI EarI Eco57MI EcoO109I HincII MscI MslI MspA1I
NaeI NcoI NgoMIV PfoI PspOMI SapI StuI StyI
TaqII TatI TstI XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BmrI BmtI BplI BpuEI
BsaAI BsaBI BsaWI BseRI BseYI BsgI BsiEI BsiWI
BsmI BspEI BspHI BsrBI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HindIII HpaI KasI KpnI MfeI MluI MmeI NarI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XbaI XhoI XmaI XmnI