(Linear) (Six-base) MAPSORT of: KIBB9788.seq  Check: 4271  from: 1  to: 585



KIBB9788.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AccI GT'mk_AC

Cuts at:      0     455     585 
   Size:        455     130
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     159     246     585 
   Size:        159      87     339

  Fragments arranged by size: 

                 339     159      87
 
AfeI AGC'GCT

Cuts at:      0     283     585 
   Size:        283     302
 
AleI CACnn'nnGTG

Cuts at:      0     112     585 
   Size:        112     473
 
AlwNI CAG_nnn'CTG

Cuts at:      0     345     482     585 
   Size:        345     137     103

  Fragments arranged by size: 

                 345     137     103
 
BanII G_rGCy'C

Cuts at:      0     580     585 
   Size:        580       5
 
BglII A'GATC_T

Cuts at:      0     257     585 
   Size:        257     328
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     303     585 
   Size:        303     282
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     376     585 
   Size:        376     209
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     447     585 
   Size:        142     305     138

  Fragments arranged by size: 

                 305     142     138
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     352     563     585 
   Size:        352     211      22

  Fragments arranged by size: 

                 352     211      22
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     147     276     444     585 
   Size:        147     129     168     141

  Fragments arranged by size: 

                 168     147     141     129
 
BseYI C'CCAG_C

Cuts at:      0     397     585 
   Size:        397     188
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     401     585 
   Size:        106     295     184

  Fragments arranged by size: 

                 295     184     106
 
Bsp1286I G_dGCh'C

Cuts at:      0     580     585 
   Size:        580       5
 
BspHI T'CATG_A

Cuts at:      0     512     585 
   Size:        512      73
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     363     370     585 
   Size:        363       7     215

  Fragments arranged by size: 

                 363     215       7
 
BstYI r'GATC_y

Cuts at:      0      58     257     585 
   Size:         58     199     328

  Fragments arranged by size: 

                 328     199      58
 
BtgI C'CryG_G

Cuts at:      0      87     585 
   Size:         87     498
 
EarI CTCTTCn'nnn_

Cuts at:      0     162     274     585 
   Size:        162     112     311

  Fragments arranged by size: 

                 311     162     112
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     159     246     376     585 
   Size:        159      87     130     209

  Fragments arranged by size: 

                 209     159     130      87
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     429     585 
   Size:        429     156
 
HaeII r_GCGC'y

Cuts at:      0     285     585 
   Size:        285     300
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     562     585 
   Size:        562      23
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      36     585 
   Size:         36     549
 
MslI CAynn'nnrTG

Cuts at:      0     112     585 
   Size:        112     473
 
NcoI C'CATG_G

Cuts at:      0      87     585 
   Size:         87     498
 
NspI r_CATG'y

Cuts at:      0     543     585 
   Size:        543      42
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     192     585 
   Size:        192     393
 
PstI C_TGCA'G

Cuts at:      0     254     374     585 
   Size:        254     120     211

  Fragments arranged by size: 

                 254     211     120
 
SfcI C'TryA_G

Cuts at:      0      45     250     370     585 
   Size:         45     205     120     215

  Fragments arranged by size: 

                 215     205     120      45
 
SmlI C'TyrA_G

Cuts at:      0     157     426     585 
   Size:        157     269     159

  Fragments arranged by size: 

                 269     159     157
 
StyI C'CwwG_G

Cuts at:      0      87     110     585 
   Size:         87      23     475

  Fragments arranged by size: 

                 475      87      23
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     325     585 
   Size:        325     260
 

 Enzymes that do cut:

     AccI     AcuI     AfeI     AleI    AlwNI    BanII    BglII     BplI
     BpmI    BpuEI     BsaI    BseRI    BseYI     BsgI Bsp1286I    BspHI
    BspMI    BstYI     BtgI     EarI  Eco57MI    EcoNI    HaeII    Hin4I
     MmeI     MslI     NcoI     NspI    PflMI     PstI     SfcI     SmlI
     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BmtI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI