Restriction map for
pF1KB9791
(Linear) (Six-base) MAPSORT of: KIBB9791.seq Check: 9291 from: 1 to: 630
KIBB9791.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 202 448 630
Size: 202 246 182
Fragments arranged by size:
246 202 182
AlwNI CAG_nnn'CTG
Cuts at: 0 305 630
Size: 305 325
ApaI G_GGCC'C
Cuts at: 0 167 630
Size: 167 463
AvaI C'yCGr_G
Cuts at: 0 131 450 630
Size: 131 319 180
Fragments arranged by size:
319 180 131
BanI G'GyrC_C
Cuts at: 0 151 214 369 630
Size: 151 63 155 261
Fragments arranged by size:
261 155 151 63
BanII G_rGCy'C
Cuts at: 0 167 630
Size: 167 463
BbvCI CC'TCA_GC
Cuts at: 0 437 610 630
Size: 437 173 20
Fragments arranged by size:
437 173 20
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 355 389 630
Size: 355 34 241
Fragments arranged by size:
355 241 34
BglI GCCn_nnn'nGGC
Cuts at: 0 563 620 630
Size: 563 57 10
Fragments arranged by size:
563 57 10
Bme1580I G_kGCm'C
Cuts at: 0 156 167 219 630
Size: 156 11 52 411
Fragments arranged by size:
411 156 52 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 47 504 630
Size: 47 457 126
Fragments arranged by size:
457 126 47
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 630
Size: 205 425
Bpu10I CC'TnA_GC
Cuts at: 0 437 610 630
Size: 437 173 20
Fragments arranged by size:
437 173 20
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 109 139 630
Size: 109 30 491
Fragments arranged by size:
491 109 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 37 598 630
Size: 25 12 561 32
Fragments arranged by size:
561 32 25 12
BseYI C'CCAG_C
Cuts at: 0 160 190 305 630
Size: 160 30 115 325
Fragments arranged by size:
325 160 115 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 585 630
Size: 239 346 45
Fragments arranged by size:
346 239 45
Bsp1286I G_dGCh'C
Cuts at: 0 156 167 219 630
Size: 156 11 52 411
Fragments arranged by size:
411 156 52 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 202 448 630
Size: 202 246 182
Fragments arranged by size:
246 202 182
BsrDI GCAATG_nn'
Cuts at: 0 88 630
Size: 88 542
BsrFI r'CCGG_y
Cuts at: 0 233 630
Size: 233 397
BstBI TT'CG_AA
Cuts at: 0 579 630
Size: 579 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 305 585 630
Size: 305 280 45
Fragments arranged by size:
305 280 45
BtsI GCAGTG_nn'
Cuts at: 0 587 630
Size: 587 43
EaeI y'GGCC_r
Cuts at: 0 221 621 630
Size: 221 400 9
Fragments arranged by size:
400 221 9
EarI CTCTTCn'nnn_
Cuts at: 0 181 630
Size: 181 449
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 385 630
Size: 385 245
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 630
Size: 205 425
EcoNI CCTnn'n_nnAGG
Cuts at: 0 184 630
Size: 184 446
EcoO109I rG'GnC_Cy
Cuts at: 0 117 164 194 630
Size: 117 47 30 436
Fragments arranged by size:
436 117 47 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 109 141 630
Size: 109 32 489
Fragments arranged by size:
489 109 32
MfeI C'AATT_G
Cuts at: 0 62 630
Size: 62 568
MspA1I CmG'CkG
Cuts at: 0 328 379 419 544 630
Size: 328 51 40 125 86
Fragments arranged by size:
328 125 86 51 40
NaeI GCC'GGC
Cuts at: 0 235 630
Size: 235 395
NgoMIV G'CCGG_C
Cuts at: 0 233 630
Size: 233 397
PasI CC'CwG_GG
Cuts at: 0 552 630
Size: 552 78
PpuMI rG'GwC_Cy
Cuts at: 0 117 630
Size: 117 513
PspOMI G'GGCC_C
Cuts at: 0 163 630
Size: 163 467
PstI C_TGCA'G
Cuts at: 0 378 630
Size: 378 252
PvuII CAG'CTG
Cuts at: 0 379 630
Size: 379 251
RsrII CG'GwC_CG
Cuts at: 0 323 630
Size: 323 307
SapI GCTCTTCn'nnn_
Cuts at: 0 181 630
Size: 181 449
ScaI AGT'ACT
Cuts at: 0 403 630
Size: 403 227
SfcI C'TryA_G
Cuts at: 0 374 630
Size: 374 256
SmaI CCC'GGG
Cuts at: 0 452 630
Size: 452 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 297 630
Size: 297 333
TatI w'GTAC_w
Cuts at: 0 401 630
Size: 401 229
XmaI C'CCGG_G
Cuts at: 0 450 630
Size: 450 180
Enzymes that do cut:
AarI AlwNI ApaI AvaI BanI BanII BbvCI BcgI
BglI Bme1580I BmrI BpmI Bpu10I BsaXI BseRI BseYI
BsgI Bsp1286I BspMI BsrDI BsrFI BstBI BtgZI BtsI
EaeI EarI EciI Eco57MI EcoNI EcoO109I Hin4I MfeI
MspA1I NaeI NgoMIV PasI PpuMI PspOMI PstI PvuII
RsrII SapI ScaI SfcI SmaI TaqII TatI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BciVI
BclI BfrBI BglII BlpI BmgBI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI
BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI ClaI
CspCI DraI DraIII DrdI EagI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MluI MmeI MscI MslI NarI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI SacI SacII SalI SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TstI Tth111I XbaI
XcmI XhoI XmnI ZraI