(Linear) (Six-base) MAPSORT of: KIBB9793.seq  Check: 4000  from: 1  to: 636



KIBB9793.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AleI CACnn'nnGTG

Cuts at:      0     154     636 
   Size:        154     482
 
ApaI G_GGCC'C

Cuts at:      0     441     636 
   Size:        441     195
 
BanII G_rGCy'C

Cuts at:      0      92     441     517     636 
   Size:         92     349      76     119

  Fragments arranged by size: 

                 349     119      92      76
 
BglI GCCn_nnn'nGGC

Cuts at:      0     224     636 
   Size:        224     412
 
Bme1580I G_kGCm'C

Cuts at:      0     441     636 
   Size:        441     195
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     469     636 
   Size:        469     167
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      43     636 
   Size:         43     593
 
BsaAI yAC'GTr

Cuts at:      0     120     636 
   Size:        120     516
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     441     471     636 
   Size:        441      30     165

  Fragments arranged by size: 

                 441     165      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      73     636 
   Size:         73     563
 
BseYI C'CCAG_C

Cuts at:      0     583     636 
   Size:        583      53
 
BsiHKAI G_wGCw'C

Cuts at:      0     517     636 
   Size:        517     119
 
BsmI GAATG_Cn'

Cuts at:      0     477     636 
   Size:        477     159
 
Bsp1286I G_dGCh'C

Cuts at:      0      92     441     517     636 
   Size:         92     349      76     119

  Fragments arranged by size: 

                 349     119      92      76
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     371     636 
   Size:        371     265
 
BtgI C'CryG_G

Cuts at:      0      23      53     170     290     431     557     636 
   Size:         23      30     117     120     141     126      79

  Fragments arranged by size: 

                 141     126     120     117      79      30      23
 
BtsI GCAGTG_nn'

Cuts at:      0     283     594     636 
   Size:        283     311      42

  Fragments arranged by size: 

                 311     283      42
 
EaeI y'GGCC_r

Cuts at:      0     270     636 
   Size:        270     366
 
EcoICRI GAG'CTC

Cuts at:      0     515     636 
   Size:        515     121
 
EcoO109I rG'GnC_Cy

Cuts at:      0       5      76     360     437     438     636 
   Size:          5      71     284      77       1     198

  Fragments arranged by size: 

                 284     198      77      71       5       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     551     636 
   Size:        551      85
 
MscI TGG'CCA

Cuts at:      0     272     636 
   Size:        272     364
 
MslI CAynn'nnrTG

Cuts at:      0     154     636 
   Size:        154     482
 
NcoI C'CATG_G

Cuts at:      0     290     431     557     636 
   Size:        290     141     126      79

  Fragments arranged by size: 

                 290     141     126      79
 
PasI CC'CwG_GG

Cuts at:      0     177     636 
   Size:        177     459
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     558     636 
   Size:        558      78
 
PfoI T'CCnGG_A

Cuts at:      0      78     636 
   Size:         78     558
 
PpuMI rG'GwC_Cy

Cuts at:      0       5      76     360     636 
   Size:          5      71     284     276

  Fragments arranged by size: 

                 284     276      71       5
 
PspOMI G'GGCC_C

Cuts at:      0     437     636 
   Size:        437     199
 
SacI G_AGCT'C

Cuts at:      0     517     636 
   Size:        517     119
 
StuI AGG'CCT

Cuts at:      0     214     636 
   Size:        214     422
 
StyI C'CwwG_G

Cuts at:      0     290     367     431     557     636 
   Size:        290      77      64     126      79

  Fragments arranged by size: 

                 290     126      79      77      64
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     441     473     636 
   Size:        441      32     163

  Fragments arranged by size: 

                 441     163      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     156     288     636 
   Size:        156     132     348

  Fragments arranged by size: 

                 348     156     132
 

 Enzymes that do cut:

     AleI     ApaI    BanII     BglI Bme1580I     BplI     BsaI    BsaAI
    BsaXI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BspMI     BtgI
     BtsI     EaeI  EcoICRI EcoO109I     FalI     MscI     MslI     NcoI
     PasI    PflMI     PfoI    PpuMI   PspOMI     SacI     StuI     StyI
     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI    BsaBI
    BsaHI    BsaWI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  Eco57MI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI