Restriction map for
pF1KB9794
(Linear) (Six-base) MAPSORT of: KIBB9794.seq Check: 5665 from: 1 to: 651
KIBB9794.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AfeI AGC'GCT
Cuts at: 0 29 651
Size: 29 622
AscI GG'CGCG_CC
Cuts at: 0 155 651
Size: 155 496
AvaI C'yCGr_G
Cuts at: 0 120 220 238 564 651
Size: 120 100 18 326 87
Fragments arranged by size:
326 120 100 87 18
BanI G'GyrC_C
Cuts at: 0 67 490 651
Size: 67 423 161
Fragments arranged by size:
423 161 67
BanII G_rGCy'C
Cuts at: 0 44 651
Size: 44 607
BbeI G_GCGC'C
Cuts at: 0 71 651
Size: 71 580
BbsI GAAGACnn'nnnn_
Cuts at: 0 620 651
Size: 620 31
BbvCI CC'TCA_GC
Cuts at: 0 644 651
Size: 644 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 516 550 651
Size: 516 34 101
Fragments arranged by size:
516 101 34
BglI GCCn_nnn'nGGC
Cuts at: 0 39 651
Size: 39 612
Bme1580I G_kGCm'C
Cuts at: 0 493 539 651
Size: 493 46 112
Fragments arranged by size:
493 112 46
BmgBI CAC'GTC
Cuts at: 0 336 651
Size: 336 315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 433 651
Size: 96 337 218
Fragments arranged by size:
337 218 96
Bpu10I CC'TnA_GC
Cuts at: 0 644 651
Size: 644 7
BsaHI Gr'CG_yC
Cuts at: 0 68 651
Size: 68 583
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 472 651
Size: 472 179
BseYI C'CCAG_C
Cuts at: 0 486 651
Size: 486 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 651
Size: 505 146
BsiEI CG_ry'CG
Cuts at: 0 408 651
Size: 408 243
Bsp1286I G_dGCh'C
Cuts at: 0 44 493 539 651
Size: 44 449 46 112
Fragments arranged by size:
449 112 46 44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 179 281 453 651
Size: 179 102 172 198
Fragments arranged by size:
198 179 172 102
BsrBI CCG'CTC
Cuts at: 0 80 651
Size: 80 571
BsrFI r'CCGG_y
Cuts at: 0 407 580 651
Size: 407 173 71
Fragments arranged by size:
407 173 71
BssHII G'CGCG_C
Cuts at: 0 155 651
Size: 155 496
BstEII G'GTnAC_C
Cuts at: 0 465 651
Size: 465 186
BtgI C'CryG_G
Cuts at: 0 127 651
Size: 127 524
BtsI GCAGTG_nn'
Cuts at: 0 639 651
Size: 639 12
EaeI y'GGCC_r
Cuts at: 0 446 651
Size: 446 205
EarI CTCTTCn'nnn_
Cuts at: 0 44 651
Size: 44 607
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 38 651
Size: 38 613
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 433 651
Size: 96 337 218
Fragments arranged by size:
337 218 96
EcoNI CCTnn'n_nnAGG
Cuts at: 0 423 498 651
Size: 423 75 153
Fragments arranged by size:
423 153 75
EcoO109I rG'GnC_Cy
Cuts at: 0 249 270 651
Size: 249 21 381
Fragments arranged by size:
381 249 21
HaeII r_GCGC'y
Cuts at: 0 31 71 119 651
Size: 31 40 48 532
Fragments arranged by size:
532 48 40 31
KasI G'GCGC_C
Cuts at: 0 67 651
Size: 67 584
MscI TGG'CCA
Cuts at: 0 448 651
Size: 448 203
MspA1I CmG'CkG
Cuts at: 0 177 193 651
Size: 177 16 458
Fragments arranged by size:
458 177 16
NaeI GCC'GGC
Cuts at: 0 582 651
Size: 582 69
NarI GG'CG_CC
Cuts at: 0 68 651
Size: 68 583
NgoMIV G'CCGG_C
Cuts at: 0 580 651
Size: 580 71
NspI r_CATG'y
Cuts at: 0 306 315 651
Size: 306 9 336
Fragments arranged by size:
336 306 9
PasI CC'CwG_GG
Cuts at: 0 500 651
Size: 500 151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 523 555 651
Size: 523 32 96
Fragments arranged by size:
523 96 32
PpuMI rG'GwC_Cy
Cuts at: 0 270 651
Size: 270 381
PspXI vC'TCGA_Gb
Cuts at: 0 564 651
Size: 564 87
RsrII CG'GwC_CG
Cuts at: 0 608 651
Size: 608 43
SfcI C'TryA_G
Cuts at: 0 393 651
Size: 393 258
SfoI GGC'GCC
Cuts at: 0 69 651
Size: 69 582
SmaI CCC'GGG
Cuts at: 0 222 651
Size: 222 429
SmlI C'TyrA_G
Cuts at: 0 564 651
Size: 564 87
SphI G_CATG'C
Cuts at: 0 306 651
Size: 306 345
StuI AGG'CCT
Cuts at: 0 418 642 651
Size: 418 224 9
Fragments arranged by size:
418 224 9
StyI C'CwwG_G
Cuts at: 0 470 651
Size: 470 181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 625 651
Size: 625 26
XhoI C'TCGA_G
Cuts at: 0 564 651
Size: 564 87
XmaI C'CCGG_G
Cuts at: 0 220 651
Size: 220 431
Enzymes that do cut:
AfeI AscI AvaI BanI BanII BbeI BbsI BbvCI
BcgI BglI Bme1580I BmgBI BpmI Bpu10I BsaHI BseRI
BseYI BsgI BsiEI Bsp1286I BspMI BsrBI BsrFI BssHII
BstEII BtgI BtsI EaeI EarI EciI Eco57MI EcoNI
EcoO109I HaeII KasI MscI MspA1I NaeI NarI NgoMIV
NspI PasI PpiI PpuMI PspXI RsrII SfcI SfoI
SmaI SmlI SphI StuI StyI TaqII XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvrII BaeI BamHI BciVI BclI
BfrBI BglII BlpI BmrI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BsrGI BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MslI NcoI NdeI NheI NotI NruI NsiI
PacI PciI PflMI PfoI PmeI PmlI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI TatI TstI Tth111I
XbaI XcmI XmnI ZraI