(Linear) (Six-base) MAPSORT of: KIBB9797.seq  Check: 5071  from: 1  to: 300



KIBB9797.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     300 
   Size:        109     191
 
AlwNI CAG_nnn'CTG

Cuts at:      0     120     300 
   Size:        120     180
 
ApoI r'AATT_y

Cuts at:      0      79     105     139     244     300 
   Size:         79      26      34     105      56

  Fragments arranged by size: 

                 105      79      56      34      26
 
AvaI C'yCGr_G

Cuts at:      0     278     300 
   Size:        278      22
 
BanII G_rGCy'C

Cuts at:      0     255     300 
   Size:        255      45
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     300 
   Size:         60     240
 
BsmI GAATG_Cn'

Cuts at:      0     258     273     300 
   Size:        258      15      27

  Fragments arranged by size: 

                 258      27      15
 
Bsp1286I G_dGCh'C

Cuts at:      0     255     300 
   Size:        255      45
 
DraI TTT'AAA

Cuts at:      0     144     171     300 
   Size:        144      27     129

  Fragments arranged by size: 

                 144     129      27
 
EarI CTCTTCn'nnn_

Cuts at:      0     215     300 
   Size:        215      85
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     300 
   Size:        109     191
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     216     248     300 
   Size:        216      32      52

  Fragments arranged by size: 

                 216      52      32
 
SfcI C'TryA_G

Cuts at:      0     159     300 
   Size:        159     141
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     AvaI    BanII     BsgI     BsmI Bsp1286I
     DraI     EarI  Eco57MI    Hin4I     SfcI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI