(Linear) (Six-base) MAPSORT of: KIBB9799.seq Check: 7662 from: 1 to: 351 KIBB9799.orf With 182 enzymes: SgfI * March 3, 2008 11:12 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 236 351 Size: 236 115 BfrBI ATG'CAT Cuts at: 0 297 351 Size: 297 54 Bpu10I CC'TnA_GC Cuts at: 0 253 351 Size: 253 98 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 167 245 351 Size: 167 78 106 Fragments arranged by size: 167 106 78 DraI TTT'AAA Cuts at: 0 183 351 Size: 183 168 EaeI y'GGCC_r Cuts at: 0 171 351 Size: 171 180 EarI CTCTTCn'nnn_ Cuts at: 0 254 264 351 Size: 254 10 87 Fragments arranged by size: 254 87 10 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 236 351 Size: 236 115 MscI TGG'CCA Cuts at: 0 173 351 Size: 173 178 NsiI A_TGCA'T Cuts at: 0 299 351 Size: 299 52 NspI r_CATG'y Cuts at: 0 45 351 Size: 45 306 StuI AGG'CCT Cuts at: 0 348 351 Size: 348 3 Enzymes that do cut: AcuI BfrBI Bpu10I BseRI DraI EaeI EarI Eco57MI MscI NsiI NspI StuI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI