(Linear) (Six-base) MAPSORT of: KIBB9803.seq  Check: 5612  from: 1  to: 600



KIBB9803.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     326     600 
   Size:        326     274
 
BamHI G'GATC_C

Cuts at:      0     510     600 
   Size:        510      90
 
BanII G_rGCy'C

Cuts at:      0     447     600 
   Size:        447     153
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     127     600 
   Size:        127     473
 
BglI GCCn_nnn'nGGC

Cuts at:      0     259     575     600 
   Size:        259     316      25

  Fragments arranged by size: 

                 316     259      25
 
Bme1580I G_kGCm'C

Cuts at:      0     320     600 
   Size:        320     280
 
BmgBI CAC'GTC

Cuts at:      0     111     600 
   Size:        111     489
 
Bpu10I CC'TnA_GC

Cuts at:      0     457     600 
   Size:        457     143
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     431     600 
   Size:        431     169
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     424     466     600 
   Size:        424      42     134

  Fragments arranged by size: 

                 424     134      42
 
BsmI GAATG_Cn'

Cuts at:      0     133     600 
   Size:        133     467
 
Bsp1286I G_dGCh'C

Cuts at:      0     320     447     600 
   Size:        320     127     153

  Fragments arranged by size: 

                 320     153     127
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     200     600 
   Size:        200     400
 
BsrGI T'GTAC_A

Cuts at:      0     504     600 
   Size:        504      96
 
BstYI r'GATC_y

Cuts at:      0     510     600 
   Size:        510      90
 
BtgI C'CryG_G

Cuts at:      0     280     600 
   Size:        280     320
 
BtsI GCAGTG_nn'

Cuts at:      0     411     600 
   Size:        411     189
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     326     600 
   Size:        326     274
 
EcoRV GAT'ATC

Cuts at:      0     588     600 
   Size:        588      12
 
HaeII r_GCGC'y

Cuts at:      0     300     600 
   Size:        300     300
 
MspA1I CmG'CkG

Cuts at:      0     282     600 
   Size:        282     318
 
SacII CC_GC'GG

Cuts at:      0     283     600 
   Size:        283     317
 
SmlI C'TyrA_G

Cuts at:      0     446     600 
   Size:        446     154
 
StyI C'CwwG_G

Cuts at:      0     365     523     600 
   Size:        365     158      77

  Fragments arranged by size: 

                 365     158      77
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     466     600 
   Size:        466     134
 
TatI w'GTAC_w

Cuts at:      0     504     600 
   Size:        504      96
 

 Enzymes that do cut:

     AcuI    BamHI    BanII     BbsI     BglI Bme1580I    BmgBI   Bpu10I
    BpuEI    BseRI     BsmI Bsp1286I    BspMI    BsrGI    BstYI     BtgI
     BtsI  Eco57MI    EcoRV    HaeII   MspA1I    SacII     SmlI     StyI
    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
     BmrI     BmtI     BplI     BpmI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI