Restriction map for
pF1KB9804
(Linear) (Six-base) MAPSORT of: KIBB9804.seq Check: 8435 from: 1 to: 669
KIBB9804.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AfeI AGC'GCT
Cuts at: 0 162 669
Size: 162 507
ApaI G_GGCC'C
Cuts at: 0 308 510 669
Size: 308 202 159
Fragments arranged by size:
308 202 159
ApoI r'AATT_y
Cuts at: 0 540 669
Size: 540 129
AvaI C'yCGr_G
Cuts at: 0 511 669
Size: 511 158
BanI G'GyrC_C
Cuts at: 0 416 574 669
Size: 416 158 95
Fragments arranged by size:
416 158 95
BanII G_rGCy'C
Cuts at: 0 259 308 510 669
Size: 259 49 202 159
Fragments arranged by size:
259 202 159 49
BbsI GAAGACnn'nnnn_
Cuts at: 0 301 669
Size: 301 368
BbvCI CC'TCA_GC
Cuts at: 0 502 669
Size: 502 167
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 136 170 669
Size: 136 34 499
Fragments arranged by size:
499 136 34
BglI GCCn_nnn'nGGC
Cuts at: 0 370 379 669
Size: 370 9 290
Fragments arranged by size:
370 290 9
Bme1580I G_kGCm'C
Cuts at: 0 308 510 669
Size: 308 202 159
Fragments arranged by size:
308 202 159
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 225 669
Size: 225 444
Bpu10I CC'TnA_GC
Cuts at: 0 502 669
Size: 502 167
BsaI GGTCTCn'nnnn_
Cuts at: 0 220 669
Size: 220 449
BsaAI yAC'GTr
Cuts at: 0 368 669
Size: 368 301
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 36 511 529 669
Size: 36 475 18 140
Fragments arranged by size:
475 140 36 18
BseYI C'CCAG_C
Cuts at: 0 551 669
Size: 551 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 372 669
Size: 250 122 297
Fragments arranged by size:
297 250 122
Bsp1286I G_dGCh'C
Cuts at: 0 259 308 510 669
Size: 259 49 202 159
Fragments arranged by size:
259 202 159 49
BsrBI CCG'CTC
Cuts at: 0 262 669
Size: 262 407
BsrDI GCAATG_nn'
Cuts at: 0 177 669
Size: 177 492
BssSI C'ACGA_G
Cuts at: 0 237 669
Size: 237 432
BstEII G'GTnAC_C
Cuts at: 0 466 669
Size: 466 203
BstXI CCAn_nnnn'nTGG
Cuts at: 0 337 669
Size: 337 332
BstYI r'GATC_y
Cuts at: 0 323 669
Size: 323 346
BtgI C'CryG_G
Cuts at: 0 212 621 669
Size: 212 409 48
Fragments arranged by size:
409 212 48
EaeI y'GGCC_r
Cuts at: 0 600 624 669
Size: 600 24 45
Fragments arranged by size:
600 45 24
EarI CTCTTCn'nnn_
Cuts at: 0 125 194 669
Size: 125 69 475
Fragments arranged by size:
475 125 69
EcoNI CCTnn'n_nnAGG
Cuts at: 0 514 669
Size: 514 155
EcoO109I rG'GnC_Cy
Cuts at: 0 305 506 507 669
Size: 305 201 1 162
Fragments arranged by size:
305 201 162 1
HaeII r_GCGC'y
Cuts at: 0 164 669
Size: 164 505
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 669
Size: 34 32 603
Fragments arranged by size:
603 34 32
MscI TGG'CCA
Cuts at: 0 602 626 669
Size: 602 24 43
Fragments arranged by size:
602 43 24
MspA1I CmG'CkG
Cuts at: 0 376 441 555 669
Size: 376 65 114 114
Fragments arranged by size:
376 114 114 65
NcoI C'CATG_G
Cuts at: 0 621 669
Size: 621 48
PasI CC'CwG_GG
Cuts at: 0 408 669
Size: 408 261
PmlI CAC'GTG
Cuts at: 0 368 669
Size: 368 301
PspOMI G'GGCC_C
Cuts at: 0 304 506 669
Size: 304 202 163
Fragments arranged by size:
304 202 163
PspXI vC'TCGA_Gb
Cuts at: 0 511 669
Size: 511 158
PstI C_TGCA'G
Cuts at: 0 440 669
Size: 440 229
SfcI C'TryA_G
Cuts at: 0 436 669
Size: 436 233
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 370 669
Size: 370 299
SmlI C'TyrA_G
Cuts at: 0 511 669
Size: 511 158
StyI C'CwwG_G
Cuts at: 0 392 479 621 669
Size: 392 87 142 48
Fragments arranged by size:
392 142 87 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 421 565 644 669
Size: 421 144 79 25
Fragments arranged by size:
421 144 79 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 259 291 460 492 669
Size: 259 32 169 32 177
Fragments arranged by size:
259 177 169 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 187 334 619 669
Size: 187 147 285 50
Fragments arranged by size:
285 187 147 50
XhoI C'TCGA_G
Cuts at: 0 511 669
Size: 511 158
Enzymes that do cut:
AfeI ApaI ApoI AvaI BanI BanII BbsI BbvCI
BcgI BglI Bme1580I BplI Bpu10I BsaI BsaAI BseRI
BseYI BsgI Bsp1286I BsrBI BsrDI BssSI BstEII BstXI
BstYI BtgI EaeI EarI EcoNI EcoO109I HaeII Hin4I
MscI MspA1I NcoI PasI PmlI PspOMI PspXI PstI
SfcI SfiI SmlI StyI TaqII TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BpmI BpuEI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrFI BsrGI BssHII BstAPI
BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI Eco57MI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MslI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI Tth111I
XbaI XmaI XmnI ZraI