Restriction map for
pF1KB9805
(Linear) (Six-base) MAPSORT of: KIBB9805.seq Check: 3315 from: 1 to: 675
KIBB9805.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AflIII A'CryG_T
Cuts at: 0 29 675
Size: 29 646
AlwNI CAG_nnn'CTG
Cuts at: 0 471 519 675
Size: 471 48 156
Fragments arranged by size:
471 156 48
ApaI G_GGCC'C
Cuts at: 0 133 605 675
Size: 133 472 70
Fragments arranged by size:
472 133 70
AvaI C'yCGr_G
Cuts at: 0 132 675
Size: 132 543
BanI G'GyrC_C
Cuts at: 0 153 485 589 675
Size: 153 332 104 86
Fragments arranged by size:
332 153 104 86
BanII G_rGCy'C
Cuts at: 0 11 133 605 675
Size: 11 122 472 70
Fragments arranged by size:
472 122 70 11
BbeI G_GCGC'C
Cuts at: 0 157 489 593 675
Size: 157 332 104 82
Fragments arranged by size:
332 157 104 82
BglI GCCn_nnn'nGGC
Cuts at: 0 85 137 675
Size: 85 52 538
Fragments arranged by size:
538 85 52
BlpI GC'TnA_GC
Cuts at: 0 307 675
Size: 307 368
Bme1580I G_kGCm'C
Cuts at: 0 133 577 605 675
Size: 133 444 28 70
Fragments arranged by size:
444 133 70 28
Bpu10I CC'TnA_GC
Cuts at: 0 176 675
Size: 176 499
BsaI GGTCTCn'nnnn_
Cuts at: 0 79 302 675
Size: 79 223 373
Fragments arranged by size:
373 223 79
BsaHI Gr'CG_yC
Cuts at: 0 154 221 486 550 590 637 675
Size: 154 67 265 64 40 47 38
Fragments arranged by size:
265 154 67 64 47 40 38
BseYI C'CCAG_C
Cuts at: 0 171 675
Size: 171 504
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 675
Size: 143 532
BsiEI CG_ry'CG
Cuts at: 0 283 675
Size: 283 392
BsiHKAI G_wGCw'C
Cuts at: 0 11 675
Size: 11 664
Bsp1286I G_dGCh'C
Cuts at: 0 11 133 577 605 675
Size: 11 122 444 28 70
Fragments arranged by size:
444 122 70 28 11
BsrBI CCG'CTC
Cuts at: 0 206 327 675
Size: 206 121 348
Fragments arranged by size:
348 206 121
BssHII G'CGCG_C
Cuts at: 0 319 675
Size: 319 356
BssSI C'ACGA_G
Cuts at: 0 664 675
Size: 664 11
BtgI C'CryG_G
Cuts at: 0 48 111 149 675
Size: 48 63 38 526
Fragments arranged by size:
526 63 48 38
DraIII CAC_nnn'GTG
Cuts at: 0 572 675
Size: 572 103
EaeI y'GGCC_r
Cuts at: 0 2 280 521 558 675
Size: 2 278 241 37 117
Fragments arranged by size:
278 241 117 37 2
EagI C'GGCC_G
Cuts at: 0 280 675
Size: 280 395
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 199 280 675
Size: 199 81 395
Fragments arranged by size:
395 199 81
EcoICRI GAG'CTC
Cuts at: 0 9 675
Size: 9 666
EcoNI CCTnn'n_nnAGG
Cuts at: 0 457 505 675
Size: 457 48 170
Fragments arranged by size:
457 170 48
EcoO109I rG'GnC_Cy
Cuts at: 0 468 516 601 602 675
Size: 468 48 85 1 73
Fragments arranged by size:
468 85 73 48 1
FspI TGC'GCA
Cuts at: 0 406 675
Size: 406 269
HaeII r_GCGC'y
Cuts at: 0 157 489 593 675
Size: 157 332 104 82
Fragments arranged by size:
332 157 104 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 280 312 362 394 675
Size: 30 250 32 50 32 281
Fragments arranged by size:
281 250 50 32 32 30
KasI G'GCGC_C
Cuts at: 0 153 485 589 675
Size: 153 332 104 86
Fragments arranged by size:
332 153 104 86
MluI A'CGCG_T
Cuts at: 0 29 675
Size: 29 646
MspA1I CmG'CkG
Cuts at: 0 113 151 194 234 294 675
Size: 113 38 43 40 60 381
Fragments arranged by size:
381 113 60 43 40 38
NarI GG'CG_CC
Cuts at: 0 154 486 590 675
Size: 154 332 104 85
Fragments arranged by size:
332 154 104 85
NotI GC'GGCC_GC
Cuts at: 0 280 675
Size: 280 395
NspI r_CATG'y
Cuts at: 0 339 675
Size: 339 336
PasI CC'CwG_GG
Cuts at: 0 597 675
Size: 597 78
PflMI CCAn_nnn'nTGG
Cuts at: 0 519 675
Size: 519 156
PfoI T'CCnGG_A
Cuts at: 0 631 675
Size: 631 44
PspOMI G'GGCC_C
Cuts at: 0 129 601 675
Size: 129 472 74
Fragments arranged by size:
472 129 74
SacI G_AGCT'C
Cuts at: 0 11 675
Size: 11 664
SacII CC_GC'GG
Cuts at: 0 114 152 675
Size: 114 38 523
Fragments arranged by size:
523 114 38
SfoI GGC'GCC
Cuts at: 0 155 487 591 675
Size: 155 332 104 84
Fragments arranged by size:
332 155 104 84
SmaI CCC'GGG
Cuts at: 0 134 675
Size: 134 541
SmlI C'TyrA_G
Cuts at: 0 10 675
Size: 10 665
SphI G_CATG'C
Cuts at: 0 339 675
Size: 339 336
SrfI GCCC'GGGC
Cuts at: 0 134 675
Size: 134 541
XmaI C'CCGG_G
Cuts at: 0 132 675
Size: 132 543
Enzymes that do cut:
AflIII AlwNI ApaI AvaI BanI BanII BbeI BglI
BlpI Bme1580I Bpu10I BsaI BsaHI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BsrBI BssHII BssSI BtgI DraIII EaeI
EagI EciI EcoICRI EcoNI EcoO109I FspI HaeII Hin4I
KasI MluI MspA1I NarI NotI NspI PasI PflMI
PfoI PspOMI SacI SacII SfoI SmaI SmlI SphI
SrfI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BmgBI BmrI BmtI BplI
BpmI BpuEI BsaAI BsaBI BsaWI BsaXI BseRI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DrdI EarI Eco57MI EcoRI
EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI
MfeI MmeI MscI MslI NaeI NcoI NdeI NgoMIV
NheI NruI NsiI PacI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII
RsrII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SnaBI SpeI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI