Restriction map for
pF1KB9806
(Linear) (Six-base) MAPSORT of: KIBB9806.seq Check: 7801 from: 1 to: 678
KIBB9806.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AflII C'TTAA_G
Cuts at: 0 193 678
Size: 193 485
AleI CACnn'nnGTG
Cuts at: 0 286 678
Size: 286 392
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 304 678
Size: 304 374
ApaI G_GGCC'C
Cuts at: 0 122 678
Size: 122 556
BanI G'GyrC_C
Cuts at: 0 36 88 280 379 678
Size: 36 52 192 99 299
Fragments arranged by size:
299 192 99 52 36
BanII G_rGCy'C
Cuts at: 0 122 520 678
Size: 122 398 158
Fragments arranged by size:
398 158 122
BbeI G_GCGC'C
Cuts at: 0 40 383 678
Size: 40 343 295
Fragments arranged by size:
343 295 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 466 678
Size: 466 212
BclI T'GATC_A
Cuts at: 0 137 678
Size: 137 541
BglI GCCn_nnn'nGGC
Cuts at: 0 34 208 373 430 649 658 678
Size: 34 174 165 57 219 9 20
Fragments arranged by size:
219 174 165 57 34 20 9
BlpI GC'TnA_GC
Cuts at: 0 101 617 678
Size: 101 516 61
Fragments arranged by size:
516 101 61
Bme1580I G_kGCm'C
Cuts at: 0 122 283 304 678
Size: 122 161 21 374
Fragments arranged by size:
374 161 122 21
BmgBI CAC'GTC
Cuts at: 0 225 678
Size: 225 453
BmrI ACTGGGnnnn_n'
Cuts at: 0 118 678
Size: 118 560
Bpu10I CC'TnA_GC
Cuts at: 0 204 678
Size: 204 474
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 678
Size: 244 434
BsaHI Gr'CG_yC
Cuts at: 0 37 380 497 678
Size: 37 343 117 181
Fragments arranged by size:
343 181 117 37
BseYI C'CCAG_C
Cuts at: 0 252 678
Size: 252 426
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 678
Size: 310 368
BsiEI CG_ry'CG
Cuts at: 0 212 678
Size: 212 466
Bsp1286I G_dGCh'C
Cuts at: 0 122 283 304 520 678
Size: 122 161 21 216 158
Fragments arranged by size:
216 161 158 122 21
BsrBI CCG'CTC
Cuts at: 0 13 678
Size: 13 665
BsrDI GCAATG_nn'
Cuts at: 0 177 678
Size: 177 501
BsrFI r'CCGG_y
Cuts at: 0 39 85 91 211 678
Size: 39 46 6 120 467
Fragments arranged by size:
467 120 46 39 6
BstXI CCAn_nnnn'nTGG
Cuts at: 0 150 678
Size: 150 528
BtgI C'CryG_G
Cuts at: 0 401 485 678
Size: 401 84 193
Fragments arranged by size:
401 193 84
BtsI GCAGTG_nn'
Cuts at: 0 458 678
Size: 458 220
DraI TTT'AAA
Cuts at: 0 324 678
Size: 324 354
EaeI y'GGCC_r
Cuts at: 0 209 678
Size: 209 469
EagI C'GGCC_G
Cuts at: 0 209 678
Size: 209 469
EarI CTCTTCn'nnn_
Cuts at: 0 508 678
Size: 508 170
EcoNI CCTnn'n_nnAGG
Cuts at: 0 196 678
Size: 196 482
EcoO109I rG'GnC_Cy
Cuts at: 0 118 119 678
Size: 118 1 559
Fragments arranged by size:
559 118 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 219 678
Size: 219 459
HaeII r_GCGC'y
Cuts at: 0 40 98 383 678
Size: 40 58 285 295
Fragments arranged by size:
295 285 58 40
KasI G'GCGC_C
Cuts at: 0 36 379 678
Size: 36 343 299
Fragments arranged by size:
343 299 36
MslI CAynn'nnrTG
Cuts at: 0 286 678
Size: 286 392
MspA1I CmG'CkG
Cuts at: 0 403 487 538 678
Size: 403 84 51 140
Fragments arranged by size:
403 140 84 51
NaeI GCC'GGC
Cuts at: 0 93 678
Size: 93 585
NarI GG'CG_CC
Cuts at: 0 37 380 678
Size: 37 343 298
Fragments arranged by size:
343 298 37
NgoMIV G'CCGG_C
Cuts at: 0 91 678
Size: 91 587
PspOMI G'GGCC_C
Cuts at: 0 118 678
Size: 118 560
PstI C_TGCA'G
Cuts at: 0 438 678
Size: 438 240
SacII CC_GC'GG
Cuts at: 0 404 488 678
Size: 404 84 190
Fragments arranged by size:
404 190 84
SapI GCTCTTCn'nnn_
Cuts at: 0 508 678
Size: 508 170
SfcI C'TryA_G
Cuts at: 0 434 678
Size: 434 244
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 208 678
Size: 208 470
SfoI GGC'GCC
Cuts at: 0 38 381 678
Size: 38 343 297
Fragments arranged by size:
343 297 38
SgrAI Cr'CCGG_yG
Cuts at: 0 39 678
Size: 39 639
SmlI C'TyrA_G
Cuts at: 0 193 259 678
Size: 193 66 419
Fragments arranged by size:
419 193 66
StuI AGG'CCT
Cuts at: 0 202 678
Size: 202 476
StyI C'CwwG_G
Cuts at: 0 143 296 530 578 678
Size: 143 153 234 48 100
Fragments arranged by size:
234 153 143 100 48
Enzymes that do cut:
AflII AleI AlfI ApaI BanI BanII BbeI BbsI
BclI BglI BlpI Bme1580I BmgBI BmrI Bpu10I BpuEI
BsaHI BseYI BsgI BsiEI Bsp1286I BsrBI BsrDI BsrFI
BstXI BtgI BtsI DraI EaeI EagI EarI EcoNI
EcoO109I FalI HaeII KasI MslI MspA1I NaeI NarI
NgoMIV PspOMI PstI SacII SapI SfcI SfiI SfoI
SgrAI SmlI StuI StyI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI
BfrBI BglII BmtI BplI BpmI BsaI BsaAI BsaBI
BsaWI BsaXI BseRI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI
EciI EcoICRI Eco57MI EcoRI EcoRV FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SalI SanDI SbfI ScaI SexAI SgfI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI