Restriction map for
pF1KB9810
(Linear) (Six-base) MAPSORT of: KIBB9810.seq Check: 9901 from: 1 to: 741
KIBB9810.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 741
Size: 550 191
AflIII A'CryG_T
Cuts at: 0 192 269 362 473 741
Size: 192 77 93 111 268
Fragments arranged by size:
268 192 111 93 77
ApaI G_GGCC'C
Cuts at: 0 146 651 741
Size: 146 505 90
Fragments arranged by size:
505 146 90
BanI G'GyrC_C
Cuts at: 0 380 741
Size: 380 361
BanII G_rGCy'C
Cuts at: 0 146 651 741
Size: 146 505 90
Fragments arranged by size:
505 146 90
BbeI G_GCGC'C
Cuts at: 0 384 741
Size: 384 357
BlpI GC'TnA_GC
Cuts at: 0 412 741
Size: 412 329
Bme1580I G_kGCm'C
Cuts at: 0 146 651 741
Size: 146 505 90
Fragments arranged by size:
505 146 90
BmgBI CAC'GTC
Cuts at: 0 272 741
Size: 272 469
BmrI ACTGGGnnnn_n'
Cuts at: 0 612 627 741
Size: 612 15 114
Fragments arranged by size:
612 114 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 349 741
Size: 148 201 392
Fragments arranged by size:
392 201 148
BsaI GGTCTCn'nnnn_
Cuts at: 0 541 741
Size: 541 200
BsaAI yAC'GTr
Cuts at: 0 195 642 741
Size: 195 447 99
Fragments arranged by size:
447 195 99
BsaHI Gr'CG_yC
Cuts at: 0 381 741
Size: 381 360
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 302 741
Size: 302 439
BsiWI C'GTAC_G
Cuts at: 0 638 741
Size: 638 103
Bsp1286I G_dGCh'C
Cuts at: 0 146 651 741
Size: 146 505 90
Fragments arranged by size:
505 146 90
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 142 741
Size: 142 599
BsrGI T'GTAC_A
Cuts at: 0 266 715 741
Size: 266 449 26
Fragments arranged by size:
449 266 26
BstBI TT'CG_AA
Cuts at: 0 188 741
Size: 188 553
BtgI C'CryG_G
Cuts at: 0 117 563 656 741
Size: 117 446 93 85
Fragments arranged by size:
446 117 93 85
BtsI GCAGTG_nn'
Cuts at: 0 473 741
Size: 473 268
EarI CTCTTCn'nnn_
Cuts at: 0 197 320 741
Size: 197 123 421
Fragments arranged by size:
421 197 123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 349 550 741
Size: 148 201 201 191
Fragments arranged by size:
201 201 191 148
EcoNI CCTnn'n_nnAGG
Cuts at: 0 502 741
Size: 502 239
EcoO109I rG'GnC_Cy
Cuts at: 0 507 648 741
Size: 507 141 93
Fragments arranged by size:
507 141 93
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 555 741
Size: 555 186
HaeII r_GCGC'y
Cuts at: 0 384 741
Size: 384 357
KasI G'GCGC_C
Cuts at: 0 380 741
Size: 380 361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 741
Size: 191 550
MslI CAynn'nnrTG
Cuts at: 0 367 741
Size: 367 374
NarI GG'CG_CC
Cuts at: 0 381 741
Size: 381 360
NcoI C'CATG_G
Cuts at: 0 117 563 656 741
Size: 117 446 93 85
Fragments arranged by size:
446 117 93 85
NspI r_CATG'y
Cuts at: 0 366 477 741
Size: 366 111 264
Fragments arranged by size:
366 264 111
PciI A'CATG_T
Cuts at: 0 362 473 741
Size: 362 111 268
Fragments arranged by size:
362 268 111
PmlI CAC'GTG
Cuts at: 0 195 741
Size: 195 546
PpuMI rG'GwC_Cy
Cuts at: 0 507 741
Size: 507 234
PspOMI G'GGCC_C
Cuts at: 0 142 647 741
Size: 142 505 94
Fragments arranged by size:
505 142 94
PstI C_TGCA'G
Cuts at: 0 468 589 741
Size: 468 121 152
Fragments arranged by size:
468 152 121
SapI GCTCTTCn'nnn_
Cuts at: 0 197 741
Size: 197 544
SfcI C'TryA_G
Cuts at: 0 464 585 741
Size: 464 121 156
Fragments arranged by size:
464 156 121
SfoI GGC'GCC
Cuts at: 0 382 741
Size: 382 359
StyI C'CwwG_G
Cuts at: 0 117 146 563 656 741
Size: 117 29 417 93 85
Fragments arranged by size:
417 117 93 85 29
TatI w'GTAC_w
Cuts at: 0 266 715 741
Size: 266 449 26
Fragments arranged by size:
449 266 26
Enzymes that do cut:
AcuI AflIII ApaI BanI BanII BbeI BlpI Bme1580I
BmgBI BmrI BpmI BsaI BsaAI BsaHI BseRI BsiWI
Bsp1286I BspMI BsrGI BstBI BtgI BtsI EarI Eco57MI
EcoNI EcoO109I FalI HaeII KasI MmeI MslI NarI
NcoI NspI PciI PmlI PpuMI PspOMI PstI SapI
SfcI SfoI StyI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BmtI BplI
Bpu10I BpuEI BsaBI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoICRI EcoRI EcoRV FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI MscI MspA1I
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI