Restriction map for
pF1KB9812
(Linear) (Six-base) MAPSORT of: KIBB9812.seq Check: 7531 from: 1 to: 771
KIBB9812.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 771
Size: 668 103
AflIII A'CryG_T
Cuts at: 0 728 771
Size: 728 43
AhdI GACnn_n'nnGTC
Cuts at: 0 514 771
Size: 514 257
ApaI G_GGCC'C
Cuts at: 0 230 771
Size: 230 541
AvaI C'yCGr_G
Cuts at: 0 206 771
Size: 206 565
BanII G_rGCy'C
Cuts at: 0 230 341 771
Size: 230 111 430
Fragments arranged by size:
430 230 111
BbsI GAAGACnn'nnnn_
Cuts at: 0 89 771
Size: 89 682
BbvCI CC'TCA_GC
Cuts at: 0 197 771
Size: 197 574
BciVI GTATCCnnnnn_n'
Cuts at: 0 710 771
Size: 710 61
BlpI GC'TnA_GC
Cuts at: 0 316 771
Size: 316 455
Bme1580I G_kGCm'C
Cuts at: 0 230 771
Size: 230 541
BmgBI CAC'GTC
Cuts at: 0 731 771
Size: 731 40
Bpu10I CC'TnA_GC
Cuts at: 0 197 771
Size: 197 574
BsaAI yAC'GTr
Cuts at: 0 369 771
Size: 369 402
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 127 157 771
Size: 127 30 614
Fragments arranged by size:
614 127 30
BseYI C'CCAG_C
Cuts at: 0 525 771
Size: 525 246
BsiHKAI G_wGCw'C
Cuts at: 0 184 678 771
Size: 184 494 93
Fragments arranged by size:
494 184 93
BsmI GAATG_Cn'
Cuts at: 0 241 771
Size: 241 530
BsmBI CGTCTCn'nnnn_
Cuts at: 0 282 771
Size: 282 489
Bsp1286I G_dGCh'C
Cuts at: 0 184 230 341 678 771
Size: 184 46 111 337 93
Fragments arranged by size:
337 184 111 93 46
BsrBI CCG'CTC
Cuts at: 0 219 771
Size: 219 552
BsrDI GCAATG_nn'
Cuts at: 0 266 771
Size: 266 505
BsrFI r'CCGG_y
Cuts at: 0 597 771
Size: 597 174
BssSI C'ACGA_G
Cuts at: 0 700 771
Size: 700 71
BstEII G'GTnAC_C
Cuts at: 0 474 771
Size: 474 297
BstXI CCAn_nnnn'nTGG
Cuts at: 0 153 771
Size: 153 618
BtgI C'CryG_G
Cuts at: 0 39 304 380 771
Size: 39 265 76 391
Fragments arranged by size:
391 265 76 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 594 771
Size: 594 177
DrdI GACnn_nn'nnGTC
Cuts at: 0 483 771
Size: 483 288
EaeI y'GGCC_r
Cuts at: 0 491 771
Size: 491 280
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 420 771
Size: 420 351
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 771
Size: 668 103
EcoO109I rG'GnC_Cy
Cuts at: 0 226 309 771
Size: 226 83 462
Fragments arranged by size:
462 226 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 472 500 504 532 771
Size: 472 28 4 28 239
Fragments arranged by size:
472 239 28 28 4
MspA1I CmG'CkG
Cuts at: 0 306 771
Size: 306 465
PpuMI rG'GwC_Cy
Cuts at: 0 309 771
Size: 309 462
PspOMI G'GGCC_C
Cuts at: 0 226 771
Size: 226 545
SacII CC_GC'GG
Cuts at: 0 307 771
Size: 307 464
ScaI AGT'ACT
Cuts at: 0 445 771
Size: 445 326
SexAI A'CCwGG_T
Cuts at: 0 512 771
Size: 512 259
SspI AAT'ATT
Cuts at: 0 565 771
Size: 565 206
StyI C'CwwG_G
Cuts at: 0 48 332 771
Size: 48 284 439
Fragments arranged by size:
439 284 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 462 493 771
Size: 462 31 278
Fragments arranged by size:
462 278 31
TatI w'GTAC_w
Cuts at: 0 443 771
Size: 443 328
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 293 325 771
Size: 293 32 446
Fragments arranged by size:
446 293 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 153 771
Size: 153 618
Enzymes that do cut:
AcuI AflIII AhdI ApaI AvaI BanII BbsI BbvCI
BciVI BlpI Bme1580I BmgBI Bpu10I BsaAI BsaXI BseYI
BsiHKAI BsmI BsmBI Bsp1286I BsrBI BsrDI BsrFI BssSI
BstEII BstXI BtgI BtgZI DrdI EaeI EciI Eco57MI
EcoO109I Hin4I MspA1I PpuMI PspOMI SacII ScaI SexAI
SspI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BcgI BclI
BfrBI BglI BglII BmrI BmtI BplI BpmI BpuEI
BsaI BsaBI BsaHI BsaWI BseRI BsgI BsiEI BsiWI
BspEI BspHI BspMI BsrGI BssHII BstAPI BstBI BstYI
BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII EagI
EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI StuI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI