Restriction map for
pF1KB9815
(Linear) (Six-base) MAPSORT of: KIBB9815.seq Check: 9896 from: 1 to: 897
KIBB9815.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 597 897
Size: 597 300
AflIII A'CryG_T
Cuts at: 0 832 897
Size: 832 65
AhdI GACnn_n'nnGTC
Cuts at: 0 372 897
Size: 372 525
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 430 462 897
Size: 430 32 435
Fragments arranged by size:
435 430 32
AlwNI CAG_nnn'CTG
Cuts at: 0 441 897
Size: 441 456
ApaI G_GGCC'C
Cuts at: 0 677 897
Size: 677 220
ApaLI G'TGCA_C
Cuts at: 0 757 897
Size: 757 140
AscI GG'CGCG_CC
Cuts at: 0 260 897
Size: 260 637
AvaI C'yCGr_G
Cuts at: 0 12 138 235 630 685 897
Size: 12 126 97 395 55 212
Fragments arranged by size:
395 212 126 97 55 12
BamHI G'GATC_C
Cuts at: 0 43 884 897
Size: 43 841 13
Fragments arranged by size:
841 43 13
BanI G'GyrC_C
Cuts at: 0 465 787 897
Size: 465 322 110
Fragments arranged by size:
465 322 110
BanII G_rGCy'C
Cuts at: 0 19 139 677 746 897
Size: 19 120 538 69 151
Fragments arranged by size:
538 151 120 69 19
BbvCI CC'TCA_GC
Cuts at: 0 70 897
Size: 70 827
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 395 429 841 875 897
Size: 395 34 412 34 22
Fragments arranged by size:
412 395 34 34 22
BglI GCCn_nnn'nGGC
Cuts at: 0 689 897
Size: 689 208
Bme1580I G_kGCm'C
Cuts at: 0 470 677 694 761 897
Size: 470 207 17 67 136
Fragments arranged by size:
470 207 136 67 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 807 897
Size: 807 90
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 765 897
Size: 765 132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 463 556 760 897
Size: 127 336 93 204 137
Fragments arranged by size:
336 204 137 127 93
Bpu10I CC'TnA_GC
Cuts at: 0 70 897
Size: 70 827
BsaAI yAC'GTr
Cuts at: 0 390 897
Size: 390 507
BsaHI Gr'CG_yC
Cuts at: 0 320 897
Size: 320 577
BseYI C'CCAG_C
Cuts at: 0 226 897
Size: 226 671
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 835 897
Size: 370 465 62
Fragments arranged by size:
465 370 62
BsiEI CG_ry'CG
Cuts at: 0 283 897
Size: 283 614
BsiHKAI G_wGCw'C
Cuts at: 0 19 746 761 897
Size: 19 727 15 136
Fragments arranged by size:
727 136 19 15
BsmBI CGTCTCn'nnnn_
Cuts at: 0 359 897
Size: 359 538
Bsp1286I G_dGCh'C
Cuts at: 0 19 139 470 677 694 746 761 897
Size: 19 120 331 207 17 52 15 136
Fragments arranged by size:
331 207 136 120 52 19 17 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 597 897
Size: 597 300
BsrDI GCAATG_nn'
Cuts at: 0 456 897
Size: 456 441
BssHII G'CGCG_C
Cuts at: 0 146 214 260 897
Size: 146 68 46 637
Fragments arranged by size:
637 146 68 46
BssSI C'ACGA_G
Cuts at: 0 526 649 897
Size: 526 123 248
Fragments arranged by size:
526 248 123
BstYI r'GATC_y
Cuts at: 0 43 884 897
Size: 43 841 13
Fragments arranged by size:
841 43 13
BtgI C'CryG_G
Cuts at: 0 20 112 254 897
Size: 20 92 142 643
Fragments arranged by size:
643 142 92 20
BtsI GCAGTG_nn'
Cuts at: 0 220 897
Size: 220 677
EaeI y'GGCC_r
Cuts at: 0 280 897
Size: 280 617
EagI C'GGCC_G
Cuts at: 0 280 897
Size: 280 617
EarI CTCTTCn'nnn_
Cuts at: 0 869 897
Size: 869 28
EcoICRI GAG'CTC
Cuts at: 0 17 744 897
Size: 17 727 153
Fragments arranged by size:
727 153 17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 463 556 760 897
Size: 127 336 93 204 137
Fragments arranged by size:
336 204 137 127 93
EcoO109I rG'GnC_Cy
Cuts at: 0 125 438 682 897
Size: 125 313 244 215
Fragments arranged by size:
313 244 215 125
HaeII r_GCGC'y
Cuts at: 0 38 169 243 272 513 897
Size: 38 131 74 29 241 384
Fragments arranged by size:
384 241 131 74 38 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 358 390 897
Size: 358 32 507
Fragments arranged by size:
507 358 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 897
Size: 62 835
MspA1I CmG'CkG
Cuts at: 0 22 74 114 256 384 519 569 897
Size: 22 52 40 142 128 135 50 328
Fragments arranged by size:
328 142 135 128 52 50 40 22
NspI r_CATG'y
Cuts at: 0 836 858 897
Size: 836 22 39
Fragments arranged by size:
836 39 22
PasI CC'CwG_GG
Cuts at: 0 39 130 324 897
Size: 39 91 194 573
Fragments arranged by size:
573 194 91 39
PciI A'CATG_T
Cuts at: 0 832 897
Size: 832 65
PflMI CCAn_nnn'nTGG
Cuts at: 0 441 897
Size: 441 456
PfoI T'CCnGG_A
Cuts at: 0 46 897
Size: 46 851
PmlI CAC'GTG
Cuts at: 0 390 897
Size: 390 507
PpuMI rG'GwC_Cy
Cuts at: 0 438 897
Size: 438 459
PspOMI G'GGCC_C
Cuts at: 0 673 897
Size: 673 224
PstI C_TGCA'G
Cuts at: 0 292 430 518 897
Size: 292 138 88 379
Fragments arranged by size:
379 292 138 88
PvuII CAG'CTG
Cuts at: 0 74 897
Size: 74 823
SacI G_AGCT'C
Cuts at: 0 19 746 897
Size: 19 727 151
Fragments arranged by size:
727 151 19
SacII CC_GC'GG
Cuts at: 0 23 115 257 897
Size: 23 92 142 640
Fragments arranged by size:
640 142 92 23
ScaI AGT'ACT
Cuts at: 0 796 897
Size: 796 101
SfcI C'TryA_G
Cuts at: 0 120 288 426 514 822 897
Size: 120 168 138 88 308 75
Fragments arranged by size:
308 168 138 120 88 75
SmaI CCC'GGG
Cuts at: 0 140 897
Size: 140 757
SrfI GCCC'GGGC
Cuts at: 0 140 897
Size: 140 757
StyI C'CwwG_G
Cuts at: 0 558 897
Size: 558 339
TatI w'GTAC_w
Cuts at: 0 794 897
Size: 794 103
XmaI C'CCGG_G
Cuts at: 0 138 897
Size: 138 759
Enzymes that do cut:
AarI AflIII AhdI AloI AlwNI ApaI ApaLI AscI
AvaI BamHI BanI BanII BbvCI BcgI BglI Bme1580I
BmrI BplI BpmI Bpu10I BsaAI BsaHI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BssHII BssSI
BstYI BtgI BtsI EaeI EagI EarI EcoICRI Eco57MI
EcoO109I HaeII Hin4I MmeI MspA1I NspI PasI PciI
PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI
SacII ScaI SfcI SmaI SrfI StyI TatI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AleI AlfI ApoI AseI AsiSI AvrII BaeI BbeI
BbsI BciVI BclI BfrBI BglII BlpI BmgBI BmtI
BpuEI BsaI BsaBI BsaWI BsaXI BseRI BsiWI BsmI
BspEI BspHI BsrBI BsrFI BsrGI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EciI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SspI StuI SwaI TaqII TstI Tth111I XbaI
XcmI XhoI XmnI ZraI