Restriction map for
pF1KB9816
(Linear) (Six-base) MAPSORT of: KIBB9816.seq Check: 4741 from: 1 to: 900
KIBB9816.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AccI GT'mk_AC
Cuts at: 0 128 900
Size: 128 772
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 534 874 900
Size: 202 332 340 26
Fragments arranged by size:
340 332 202 26
AgeI A'CCGG_T
Cuts at: 0 826 900
Size: 826 74
ApaI G_GGCC'C
Cuts at: 0 396 687 900
Size: 396 291 213
Fragments arranged by size:
396 291 213
BanI G'GyrC_C
Cuts at: 0 793 900
Size: 793 107
BanII G_rGCy'C
Cuts at: 0 396 687 785 900
Size: 396 291 98 115
Fragments arranged by size:
396 291 115 98
BbsI GAAGACnn'nnnn_
Cuts at: 0 234 862 900
Size: 234 628 38
Fragments arranged by size:
628 234 38
BglI GCCn_nnn'nGGC
Cuts at: 0 208 391 541 673 682 900
Size: 208 183 150 132 9 218
Fragments arranged by size:
218 208 183 150 132 9
Bme1580I G_kGCm'C
Cuts at: 0 238 396 624 687 900
Size: 238 158 228 63 213
Fragments arranged by size:
238 228 213 158 63
BmtI G_CTAG'C
Cuts at: 0 202 900
Size: 202 698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 422 532 819 900
Size: 422 110 287 81
Fragments arranged by size:
422 287 110 81
BsaAI yAC'GTr
Cuts at: 0 606 900
Size: 606 294
BsaWI w'CCGG_w
Cuts at: 0 350 501 826 900
Size: 350 151 325 74
Fragments arranged by size:
350 325 151 74
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 709 739 900
Size: 709 30 161
Fragments arranged by size:
709 161 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 679 900
Size: 679 221
BseYI C'CCAG_C
Cuts at: 0 51 777 900
Size: 51 726 123
Fragments arranged by size:
726 123 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 900
Size: 323 577
BsmBI CGTCTCn'nnnn_
Cuts at: 0 115 900
Size: 115 785
Bsp1286I G_dGCh'C
Cuts at: 0 238 396 624 687 785 900
Size: 238 158 228 63 98 115
Fragments arranged by size:
238 228 158 115 98 63
BspEI T'CCGG_A
Cuts at: 0 350 900
Size: 350 550
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 14 164 900
Size: 14 150 736
Fragments arranged by size:
736 150 14
BsrBI CCG'CTC
Cuts at: 0 63 632 900
Size: 63 569 268
Fragments arranged by size:
569 268 63
BsrFI r'CCGG_y
Cuts at: 0 222 274 486 535 676 826 900
Size: 222 52 212 49 141 150 74
Fragments arranged by size:
222 212 150 141 74 52 49
BstEII G'GTnAC_C
Cuts at: 0 822 900
Size: 822 78
BstXI CCAn_nnnn'nTGG
Cuts at: 0 446 900
Size: 446 454
EaeI y'GGCC_r
Cuts at: 0 38 74 290 383 584 674 734 900
Size: 38 36 216 93 201 90 60 166
Fragments arranged by size:
216 201 166 93 90 60 38 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 666 786 900
Size: 666 120 114
Fragments arranged by size:
666 120 114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 422 532 534 819 874 900
Size: 202 220 110 2 285 55 26
Fragments arranged by size:
285 220 202 110 55 26 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 351 900
Size: 351 549
EcoO109I rG'GnC_Cy
Cuts at: 0 209 393 522 683 684 712 844 900
Size: 209 184 129 161 1 28 132 56
Fragments arranged by size:
209 184 161 132 129 56 28 1
HaeII r_GCGC'y
Cuts at: 0 199 900
Size: 199 701
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 156 188 514 546 900
Size: 156 32 326 32 354
Fragments arranged by size:
354 326 156 32 32
HincII GTy'rAC
Cuts at: 0 492 900
Size: 492 408
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 410 752 900
Size: 176 234 342 148
Fragments arranged by size:
342 234 176 148
MscI TGG'CCA
Cuts at: 0 76 586 736 900
Size: 76 510 150 164
Fragments arranged by size:
510 164 150 76
MspA1I CmG'CkG
Cuts at: 0 819 900
Size: 819 81
NaeI GCC'GGC
Cuts at: 0 224 276 537 678 900
Size: 224 52 261 141 222
Fragments arranged by size:
261 224 222 141 52
NgoMIV G'CCGG_C
Cuts at: 0 222 274 535 676 900
Size: 222 52 261 141 224
Fragments arranged by size:
261 224 222 141 52
NheI G'CTAG_C
Cuts at: 0 198 900
Size: 198 702
PasI CC'CwG_GG
Cuts at: 0 471 900
Size: 471 429
PfoI T'CCnGG_A
Cuts at: 0 438 900
Size: 438 462
PpuMI rG'GwC_Cy
Cuts at: 0 522 712 844 900
Size: 522 190 132 56
Fragments arranged by size:
522 190 132 56
PspOMI G'GGCC_C
Cuts at: 0 392 683 900
Size: 392 291 217
Fragments arranged by size:
392 291 217
SanDI GG'GwC_CC
Cuts at: 0 522 900
Size: 522 378
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 391 682 900
Size: 391 291 218
Fragments arranged by size:
391 291 218
StuI AGG'CCT
Cuts at: 0 517 900
Size: 517 383
StyI C'CwwG_G
Cuts at: 0 332 581 900
Size: 332 249 319
Fragments arranged by size:
332 319 249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 660 900
Size: 660 240
Enzymes that do cut:
AccI AcuI AgeI ApaI BanI BanII BbsI BglI
Bme1580I BmtI BpmI BsaAI BsaWI BsaXI BseRI BseYI
BsgI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BstEII
BstXI EaeI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I
HincII MmeI MscI MspA1I NaeI NgoMIV NheI PasI
PfoI PpuMI PspOMI SanDI SfiI StuI StyI TaqII
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BplI
Bpu10I BpuEI BsaI BsaBI BsaHI BsiEI BsiHKAI BsiWI
BsmI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EarI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI MslI NarI NcoI NdeI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI