(Linear) (Six-base) MAPSORT of: KIBB9817.seq  Check: 4029  from: 1  to: 915



KIBB9817.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     329     915 
   Size:        329     586
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     457     915 
   Size:        457     458
 
AflIII A'CryG_T

Cuts at:      0     824     915 
   Size:        824      91
 
AhdI GACnn_n'nnGTC

Cuts at:      0     503     915 
   Size:        503     412
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     614     915 
   Size:        614     301
 
AlwNI CAG_nnn'CTG

Cuts at:      0     876     915 
   Size:        876      39
 
ApaLI G'TGCA_C

Cuts at:      0     686     915 
   Size:        686     229
 
ApoI r'AATT_y

Cuts at:      0     417     604     915 
   Size:        417     187     311

  Fragments arranged by size: 

                 417     311     187
 
AseI AT'TA_AT

Cuts at:      0     455     915 
   Size:        455     460
 
AvrII C'CTAG_G

Cuts at:      0     510     915 
   Size:        510     405
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     653     915 
   Size:        653     262
 
Bme1580I G_kGCm'C

Cuts at:      0     690     915 
   Size:        690     225
 
BmgBI CAC'GTC

Cuts at:      0     825     915 
   Size:        825      90
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      99     287     915 
   Size:         99     188     628

  Fragments arranged by size: 

                 628     188      99
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     915 
   Size:        106     809
 
BsaAI yAC'GTr

Cuts at:      0     550     915 
   Size:        550     365
 
BsiHKAI G_wGCw'C

Cuts at:      0     690     915 
   Size:        690     225
 
BsmI GAATG_Cn'

Cuts at:      0      24     733     915 
   Size:         24     709     182

  Fragments arranged by size: 

                 709     182      24
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     511     915 
   Size:        511     404
 
Bsp1286I G_dGCh'C

Cuts at:      0     690     915 
   Size:        690     225
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     329     915 
   Size:        329     586
 
BsrGI T'GTAC_A

Cuts at:      0     325     915 
   Size:        325     590
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     728     915 
   Size:        728     187
 
BstBI TT'CG_AA

Cuts at:      0     372     915 
   Size:        372     543
 
BtsI GCAGTG_nn'

Cuts at:      0     415     915 
   Size:        415     500
 
DraI TTT'AAA

Cuts at:      0     132     915 
   Size:        132     783
 
EarI CTCTTCn'nnn_

Cuts at:      0      19     915 
   Size:         19     896
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     457     915 
   Size:        106     351     458

  Fragments arranged by size: 

                 458     351     106
 
EcoO109I rG'GnC_Cy

Cuts at:      0      81     915 
   Size:         81     834
 
EcoRV GAT'ATC

Cuts at:      0     283     915 
   Size:        283     632
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     188     220     489     521     915 
   Size:        188      32     269      32     394

  Fragments arranged by size: 

                 394     269     188      32      32
 
MfeI C'AATT_G

Cuts at:      0     329     915 
   Size:        329     586
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      38     915 
   Size:         38     877
 
PstI C_TGCA'G

Cuts at:      0     872     915 
   Size:        872      43
 
SfcI C'TryA_G

Cuts at:      0     868     915 
   Size:        868      47
 
SspI AAT'ATT

Cuts at:      0     246     915 
   Size:        246     669
 
StyI C'CwwG_G

Cuts at:      0     510     915 
   Size:        510     405
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     306     915 
   Size:        306     609
 
TatI w'GTAC_w

Cuts at:      0     251     325     827     858     915 
   Size:        251      74     502      31      57

  Fragments arranged by size: 

                 502     251      74      57      31
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AhdI     AlfI    AlwNI    ApaLI     ApoI
     AseI    AvrII     BbsI Bme1580I    BmgBI     BmrI     BpmI    BsaAI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrGI   BstAPI    BstBI
     BtsI     DraI     EarI  Eco57MI EcoO109I    EcoRV    Hin4I     MfeI
     MmeI     PstI     SfcI     SspI     StyI    TaqII     TatI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AloI     ApaI     AscI    AsiSI     AvaI     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MluI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI