Restriction map for
pF1KB9818
(Linear) (Six-base) MAPSORT of: KIBB9818.seq Check: 4200 from: 1 to: 927
KIBB9818.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 526 747 927
Size: 526 221 180
Fragments arranged by size:
526 221 180
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 274 306 927
Size: 274 32 621
Fragments arranged by size:
621 274 32
ApaI G_GGCC'C
Cuts at: 0 810 927
Size: 810 117
AvrII C'CTAG_G
Cuts at: 0 522 927
Size: 522 405
BanI G'GyrC_C
Cuts at: 0 121 135 759 927
Size: 121 14 624 168
Fragments arranged by size:
624 168 121 14
BanII G_rGCy'C
Cuts at: 0 704 810 927
Size: 704 106 117
Fragments arranged by size:
704 117 106
BciVI GTATCCnnnnn_n'
Cuts at: 0 286 927
Size: 286 641
BclI T'GATC_A
Cuts at: 0 848 927
Size: 848 79
BglI GCCn_nnn'nGGC
Cuts at: 0 49 927
Size: 49 878
BglII A'GATC_T
Cuts at: 0 425 927
Size: 425 502
Bme1580I G_kGCm'C
Cuts at: 0 124 140 810 927
Size: 124 16 670 117
Fragments arranged by size:
670 124 117 16
BmgBI CAC'GTC
Cuts at: 0 474 927
Size: 474 453
BmrI ACTGGGnnnn_n'
Cuts at: 0 221 927
Size: 221 706
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 301 406 766 927
Size: 148 153 105 360 161
Fragments arranged by size:
360 161 153 148 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 67 859 927
Size: 28 39 792 68
Fragments arranged by size:
792 68 39 28
BsaI GGTCTCn'nnnn_
Cuts at: 0 532 927
Size: 532 395
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 144 174 274 304 927
Size: 144 30 100 30 623
Fragments arranged by size:
623 144 100 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 30 87 90 93 98 145 268 331
Size: 30 57 3 3 5 47 123 63
Cuts at: 331 646 661 925 927
Size: 315 15 264 2
Fragments arranged by size:
315 264 123 63 57 47 30 15
5 3 3 2
BseYI C'CCAG_C
Cuts at: 0 145 546 927
Size: 145 401 381
Fragments arranged by size:
401 381 145
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 103 751 927
Size: 7 96 648 176
Fragments arranged by size:
648 176 96 7
BsiHKAI G_wGCw'C
Cuts at: 0 686 927
Size: 686 241
Bsp1286I G_dGCh'C
Cuts at: 0 124 140 686 704 810 927
Size: 124 16 546 18 106 117
Fragments arranged by size:
546 124 117 106 18 16
BspHI T'CATG_A
Cuts at: 0 599 927
Size: 599 328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 526 747 927
Size: 526 221 180
Fragments arranged by size:
526 221 180
BsrDI GCAATG_nn'
Cuts at: 0 614 927
Size: 614 313
BsrFI r'CCGG_y
Cuts at: 0 493 752 927
Size: 493 259 175
Fragments arranged by size:
493 259 175
BstYI r'GATC_y
Cuts at: 0 156 425 927
Size: 156 269 502
Fragments arranged by size:
502 269 156
Bsu36I CC'TnA_GG
Cuts at: 0 35 173 629 927
Size: 35 138 456 298
Fragments arranged by size:
456 298 138 35
BtsI GCAGTG_nn'
Cuts at: 0 24 140 927
Size: 24 116 787
Fragments arranged by size:
787 116 24
EaeI y'GGCC_r
Cuts at: 0 495 618 927
Size: 495 123 309
Fragments arranged by size:
495 309 123
EarI CTCTTCn'nnn_
Cuts at: 0 245 911 927
Size: 245 666 16
Fragments arranged by size:
666 245 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 174 927
Size: 174 753
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 301 406 766 927
Size: 148 153 105 360 161
Fragments arranged by size:
360 161 153 148 105
EcoNI CCTnn'n_nnAGG
Cuts at: 0 130 927
Size: 130 797
EcoO109I rG'GnC_Cy
Cuts at: 0 63 806 807 927
Size: 63 743 1 120
Fragments arranged by size:
743 120 63 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 287 594 927
Size: 287 307 333
Fragments arranged by size:
333 307 287
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 287 319 927
Size: 287 32 608
Fragments arranged by size:
608 287 32
HincII GTy'rAC
Cuts at: 0 152 927
Size: 152 775
MslI CAynn'nnrTG
Cuts at: 0 472 503 927
Size: 472 31 424
Fragments arranged by size:
472 424 31
MspA1I CmG'CkG
Cuts at: 0 456 927
Size: 456 471
PasI CC'CwG_GG
Cuts at: 0 66 927
Size: 66 861
PflMI CCAn_nnn'nTGG
Cuts at: 0 726 927
Size: 726 201
PfoI T'CCnGG_A
Cuts at: 0 278 927
Size: 278 649
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 274 306 927
Size: 274 32 621
Fragments arranged by size:
621 274 32
PspOMI G'GGCC_C
Cuts at: 0 806 927
Size: 806 121
PvuII CAG'CTG
Cuts at: 0 456 927
Size: 456 471
SmlI C'TyrA_G
Cuts at: 0 7 46 838 927
Size: 7 39 792 89
Fragments arranged by size:
792 89 39 7
StyI C'CwwG_G
Cuts at: 0 522 802 927
Size: 522 280 125
Fragments arranged by size:
522 280 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 538 733 927
Size: 538 195 194
Fragments arranged by size:
538 195 194
Enzymes that do cut:
AarI AloI ApaI AvrII BanI BanII BciVI BclI
BglI BglII Bme1580I BmgBI BmrI BpmI BpuEI BsaI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI
BsrDI BsrFI BstYI Bsu36I BtsI EaeI EarI EciI
Eco57MI EcoNI EcoO109I FalI Hin4I HincII MslI MspA1I
PasI PflMI PfoI PpiI PspOMI PvuII SmlI StyI
TaqII
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI BaeI BamHI BbeI BbsI BbvCI
BcgI BfrBI BlpI BmtI BplI Bpu10I BsaAI BsaBI
BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI
EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI