Restriction map for
pF1KB9825
(Linear) (Six-base) MAPSORT of: KIBB9825.seq Check: 370 from: 1 to: 951
KIBB9825.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 550 951
Size: 550 401
Acc65I G'GTAC_C
Cuts at: 0 783 951
Size: 783 168
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 951
Size: 496 455
AfeI AGC'GCT
Cuts at: 0 436 951
Size: 436 515
AhdI GACnn_n'nnGTC
Cuts at: 0 306 951
Size: 306 645
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 128 951
Size: 128 823
BanI G'GyrC_C
Cuts at: 0 145 783 951
Size: 145 638 168
Fragments arranged by size:
638 168 145
BanII G_rGCy'C
Cuts at: 0 728 834 951
Size: 728 106 117
Fragments arranged by size:
728 117 106
BglII A'GATC_T
Cuts at: 0 584 951
Size: 584 367
Bme1580I G_kGCm'C
Cuts at: 0 625 951
Size: 625 326
BmgBI CAC'GTC
Cuts at: 0 498 951
Size: 498 453
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 343 951
Size: 343 608
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 261 430 790 883 951
Size: 172 89 169 360 93 68
Fragments arranged by size:
360 172 169 93 89 68
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 111 114 115 117 122 292 670 685
Size: 111 3 1 2 5 170 378 15
Cuts at: 685 949 951
Size: 264 2
Fragments arranged by size:
378 264 170 111 15 5 3 2
2 1
BseYI C'CCAG_C
Cuts at: 0 570 675 951
Size: 570 105 276
Fragments arranged by size:
570 276 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 951
Size: 775 176
BsiHKAI G_wGCw'C
Cuts at: 0 710 951
Size: 710 241
Bsp1286I G_dGCh'C
Cuts at: 0 625 710 728 834 951
Size: 625 85 18 106 117
Fragments arranged by size:
625 117 106 85 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 169 550 771 951
Size: 169 381 221 180
Fragments arranged by size:
381 221 180 169
BsrDI GCAATG_nn'
Cuts at: 0 638 896 951
Size: 638 258 55
Fragments arranged by size:
638 258 55
BsrFI r'CCGG_y
Cuts at: 0 776 951
Size: 776 175
BssHII G'CGCG_C
Cuts at: 0 802 951
Size: 802 149
BstXI CCAn_nnnn'nTGG
Cuts at: 0 387 951
Size: 387 564
BstYI r'GATC_y
Cuts at: 0 584 951
Size: 584 367
Bsu36I CC'TnA_GG
Cuts at: 0 35 197 951
Size: 35 162 754
Fragments arranged by size:
754 162 35
BtsI GCAGTG_nn'
Cuts at: 0 24 951
Size: 24 927
EarI CTCTTCn'nnn_
Cuts at: 0 10 53 269 935 951
Size: 10 43 216 666 16
Fragments arranged by size:
666 216 43 16 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 261 430 496 790 883 951
Size: 172 89 169 66 294 93 68
Fragments arranged by size:
294 172 169 93 89 68 66
EcoNI CCTnn'n_nnAGG
Cuts at: 0 38 951
Size: 38 913
EcoO109I rG'GnC_Cy
Cuts at: 0 63 181 951
Size: 63 118 770
Fragments arranged by size:
770 118 63
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 456 501 951
Size: 456 45 450
Fragments arranged by size:
456 450 45
FspI TGC'GCA
Cuts at: 0 83 951
Size: 83 868
HaeII r_GCGC'y
Cuts at: 0 438 650 951
Size: 438 212 301
Fragments arranged by size:
438 301 212
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 292 324 634 666 951
Size: 292 32 310 32 285
Fragments arranged by size:
310 292 285 32 32
KpnI G_GTAC'C
Cuts at: 0 787 951
Size: 787 164
MfeI C'AATT_G
Cuts at: 0 626 951
Size: 626 325
MslI CAynn'nnrTG
Cuts at: 0 496 951
Size: 496 455
NspI r_CATG'y
Cuts at: 0 871 951
Size: 871 80
PasI CC'CwG_GG
Cuts at: 0 66 951
Size: 66 885
PfoI T'CCnGG_A
Cuts at: 0 860 951
Size: 860 91
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 298 330 951
Size: 298 32 621
Fragments arranged by size:
621 298 32
PpuMI rG'GwC_Cy
Cuts at: 0 181 951
Size: 181 770
StuI AGG'CCT
Cuts at: 0 602 951
Size: 602 349
StyI C'CwwG_G
Cuts at: 0 380 826 951
Size: 380 446 125
Fragments arranged by size:
446 380 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 67 667 741 757 951
Size: 67 600 74 16 194
Fragments arranged by size:
600 194 74 67 16
Enzymes that do cut:
AarI Acc65I AcuI AfeI AhdI AlfI BanI BanII
BglII Bme1580I BmgBI BplI BpmI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrDI BsrFI BssHII BstXI BstYI
Bsu36I BtsI EarI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I KpnI MfeI MslI NspI PasI PfoI
PpiI PpuMI StuI StyI TaqII
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AgeI AleI AloI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI BmrI BmtI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrGI BssSI BstAPI
BstBI BstEII BstZ17I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoRI EcoRV
FseI FspAI HincII HindIII HpaI KasI MluI MmeI
MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PflMI PmeI PmlI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI