Restriction map for
pF1KB9829
(Linear) (Six-base) MAPSORT of: KIBB9829.seq Check: 8915 from: 1 to: 1020
KIBB9829.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 324 1020
Size: 324 696
AccI GT'mk_AC
Cuts at: 0 615 1020
Size: 615 405
AflIII A'CryG_T
Cuts at: 0 277 1020
Size: 277 743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 157 280 356 1020
Size: 157 123 76 664
Fragments arranged by size:
664 157 123 76
ApaI G_GGCC'C
Cuts at: 0 172 780 1020
Size: 172 608 240
Fragments arranged by size:
608 240 172
AvaI C'yCGr_G
Cuts at: 0 148 1020
Size: 148 872
BanI G'GyrC_C
Cuts at: 0 388 703 741 1020
Size: 388 315 38 279
Fragments arranged by size:
388 315 279 38
BanII G_rGCy'C
Cuts at: 0 72 82 172 780 1020
Size: 72 10 90 608 240
Fragments arranged by size:
608 240 90 72 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 160 280 432 528 1020
Size: 160 120 152 96 492
Fragments arranged by size:
492 160 152 120 96
BclI T'GATC_A
Cuts at: 0 44 1020
Size: 44 976
BglI GCCn_nnn'nGGC
Cuts at: 0 9 428 1020
Size: 9 419 592
Fragments arranged by size:
592 419 9
Bme1580I G_kGCm'C
Cuts at: 0 172 393 708 746 780 1020
Size: 172 221 315 38 34 240
Fragments arranged by size:
315 240 221 172 38 34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 546 1020
Size: 546 474
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 742 1020
Size: 742 278
Bpu10I CC'TnA_GC
Cuts at: 0 116 344 521 1020
Size: 116 228 177 499
Fragments arranged by size:
499 228 177 116
BsaAI yAC'GTr
Cuts at: 0 280 1020
Size: 280 740
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 979 1009 1020
Size: 979 30 11
Fragments arranged by size:
979 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 574 1020
Size: 574 446
BseYI C'CCAG_C
Cuts at: 0 58 120 423 454 1020
Size: 58 62 303 31 566
Fragments arranged by size:
566 303 62 58 31
BsiHKAI G_wGCw'C
Cuts at: 0 365 465 974 1020
Size: 365 100 509 46
Fragments arranged by size:
509 365 100 46
BsmI GAATG_Cn'
Cuts at: 0 681 1020
Size: 681 339
Bsp1286I G_dGCh'C
Cuts at: 0 72 82 172 365 393 465 708 746
Size: 72 10 90 193 28 72 243 38
Cuts at: 746 780 974 1020
Size: 34 194 46
Fragments arranged by size:
243 194 193 90 72 72 46 38
34 28 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 324 1020
Size: 324 696
BsrDI GCAATG_nn'
Cuts at: 0 664 1020
Size: 664 356
BsrFI r'CCGG_y
Cuts at: 0 329 1020
Size: 329 691
BstAPI GCAn_nnn'nTGC
Cuts at: 0 666 1020
Size: 666 354
BstXI CCAn_nnnn'nTGG
Cuts at: 0 636 1020
Size: 636 384
Bsu36I CC'TnA_GG
Cuts at: 0 173 1020
Size: 173 847
BtgI C'CryG_G
Cuts at: 0 50 1020
Size: 50 970
BtsI GCAGTG_nn'
Cuts at: 0 216 1020
Size: 216 804
DraIII CAC_nnn'GTG
Cuts at: 0 469 773 1020
Size: 469 304 247
Fragments arranged by size:
469 304 247
EaeI y'GGCC_r
Cuts at: 0 10 1020
Size: 10 1010
EarI CTCTTCn'nnn_
Cuts at: 0 935 1020
Size: 935 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 742 1020
Size: 742 278
EcoO109I rG'GnC_Cy
Cuts at: 0 169 777 910 945 1020
Size: 169 608 133 35 75
Fragments arranged by size:
608 169 133 75 35
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 964 1020
Size: 964 56
HaeII r_GCGC'y
Cuts at: 0 302 485 987 1020
Size: 302 183 502 33
Fragments arranged by size:
502 302 183 33
HincII GTy'rAC
Cuts at: 0 616 1020
Size: 616 404
HindIII A'AGCT_T
Cuts at: 0 631 1020
Size: 631 389
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 1020
Size: 887 133
MscI TGG'CCA
Cuts at: 0 12 1020
Size: 12 1008
MspA1I CmG'CkG
Cuts at: 0 58 264 399 427 458 1020
Size: 58 206 135 28 31 562
Fragments arranged by size:
562 206 135 58 31 28
NcoI C'CATG_G
Cuts at: 0 50 1020
Size: 50 970
NspI r_CATG'y
Cuts at: 0 882 907 1020
Size: 882 25 113
Fragments arranged by size:
882 113 25
PasI CC'CwG_GG
Cuts at: 0 564 804 1020
Size: 564 240 216
Fragments arranged by size:
564 240 216
PflMI CCAn_nnn'nTGG
Cuts at: 0 773 1020
Size: 773 247
PfoI T'CCnGG_A
Cuts at: 0 977 1020
Size: 977 43
PmlI CAC'GTG
Cuts at: 0 280 1020
Size: 280 740
PspOMI G'GGCC_C
Cuts at: 0 168 776 1020
Size: 168 608 244
Fragments arranged by size:
608 244 168
PstI C_TGCA'G
Cuts at: 0 546 558 1020
Size: 546 12 462
Fragments arranged by size:
546 462 12
PvuII CAG'CTG
Cuts at: 0 58 399 427 1020
Size: 58 341 28 593
Fragments arranged by size:
593 341 58 28
SalI G'TCGA_C
Cuts at: 0 614 1020
Size: 614 406
SbfI CC_TGCA'GG
Cuts at: 0 546 1020
Size: 546 474
SexAI A'CCwGG_T
Cuts at: 0 384 866 1020
Size: 384 482 154
Fragments arranged by size:
482 384 154
SfcI C'TryA_G
Cuts at: 0 542 554 593 687 1020
Size: 542 12 39 94 333
Fragments arranged by size:
542 333 94 39 12
SmlI C'TyrA_G
Cuts at: 0 148 1020
Size: 148 872
SphI G_CATG'C
Cuts at: 0 907 1020
Size: 907 113
StuI AGG'CCT
Cuts at: 0 519 1020
Size: 519 501
StyI C'CwwG_G
Cuts at: 0 50 1020
Size: 50 970
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 691 804 832 1020
Size: 691 113 28 188
Fragments arranged by size:
691 188 113 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 868 900 979 1011 1020
Size: 868 32 79 32 9
Fragments arranged by size:
868 79 32 32 9
Tth111I GACn'n_nGTC
Cuts at: 0 868 1020
Size: 868 152
XhoI C'TCGA_G
Cuts at: 0 148 1020
Size: 148 872
Enzymes that do cut:
AarI AccI AflIII AlfI ApaI AvaI BanI BanII
BbsI BclI BglI Bme1580I BplI BpmI Bpu10I BsaAI
BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BsrFI BstAPI BstXI Bsu36I BtgI BtsI DraIII EaeI
EarI Eco57MI EcoO109I FalI HaeII HincII HindIII MmeI
MscI MspA1I NcoI NspI PasI PflMI PfoI PmlI
PspOMI PstI PvuII SalI SbfI SexAI SfcI SmlI
SphI StuI StyI TaqII TstI Tth111I XhoI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI
BglII BlpI BmgBI BmrI BmtI BpuEI BsaI BsaBI
BsaHI BsaWI BsgI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I
BtgZI ClaI CspCI DraI DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HpaI
KasI KpnI MfeI MluI MslI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SanDI SapI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TatI
XbaI XcmI XmaI XmnI ZraI