Restriction map for
pF1KB9832
(Linear) (Six-base) MAPSORT of: KIBB9832.seq Check: 5538 from: 1 to: 1029
KIBB9832.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 203 340 1029
Size: 175 28 137 689
Fragments arranged by size:
689 175 137 28
AfeI AGC'GCT
Cuts at: 0 188 1029
Size: 188 841
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 536 1029
Size: 536 493
AlwNI CAG_nnn'CTG
Cuts at: 0 72 1029
Size: 72 957
ApoI r'AATT_y
Cuts at: 0 150 578 802 1029
Size: 150 428 224 227
Fragments arranged by size:
428 227 224 150
AvaI C'yCGr_G
Cuts at: 0 5 1029
Size: 5 1024
BamHI G'GATC_C
Cuts at: 0 501 714 1029
Size: 501 213 315
Fragments arranged by size:
501 315 213
BanI G'GyrC_C
Cuts at: 0 250 1029
Size: 250 779
BanII G_rGCy'C
Cuts at: 0 201 825 966 1029
Size: 201 624 141 63
Fragments arranged by size:
624 201 141 63
BbvCI CC'TCA_GC
Cuts at: 0 296 1029
Size: 296 733
BglI GCCn_nnn'nGGC
Cuts at: 0 94 1029
Size: 94 935
Bme1580I G_kGCm'C
Cuts at: 0 33 642 779 1029
Size: 33 609 137 250
Fragments arranged by size:
609 250 137 33
BmgBI CAC'GTC
Cuts at: 0 1004 1029
Size: 1004 25
BmrI ACTGGGnnnn_n'
Cuts at: 0 619 1029
Size: 619 410
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 129 1029
Size: 129 900
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 874 1029
Size: 781 93 155
Fragments arranged by size:
781 155 93
Bpu10I CC'TnA_GC
Cuts at: 0 296 1029
Size: 296 733
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 270 430 1029
Size: 270 160 599
Fragments arranged by size:
599 270 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 1029
Size: 766 263
BsiHKAI G_wGCw'C
Cuts at: 0 464 825 1029
Size: 464 361 204
Fragments arranged by size:
464 361 204
Bsp1286I G_dGCh'C
Cuts at: 0 33 201 464 642 779 825 966 1029
Size: 33 168 263 178 137 46 141 63
Fragments arranged by size:
263 178 168 141 137 63 46 33
BspHI T'CATG_A
Cuts at: 0 728 1029
Size: 728 301
BstAPI GCAn_nnn'nTGC
Cuts at: 0 351 1029
Size: 351 678
BstXI CCAn_nnnn'nTGG
Cuts at: 0 741 1029
Size: 741 288
BstYI r'GATC_y
Cuts at: 0 69 312 440 501 714 1029
Size: 69 243 128 61 213 315
Fragments arranged by size:
315 243 213 128 69 61
BtgI C'CryG_G
Cuts at: 0 977 1029
Size: 977 52
DraI TTT'AAA
Cuts at: 0 320 1029
Size: 320 709
DraIII CAC_nnn'GTG
Cuts at: 0 481 613 1029
Size: 481 132 416
Fragments arranged by size:
481 416 132
EarI CTCTTCn'nnn_
Cuts at: 0 10 641 905 1029
Size: 10 631 264 124
Fragments arranged by size:
631 264 124 10
EcoICRI GAG'CTC
Cuts at: 0 823 1029
Size: 823 206
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 203 340 781 874 1029
Size: 175 28 137 441 93 155
Fragments arranged by size:
441 175 155 137 93 28
EcoRI G'AATT_C
Cuts at: 0 802 1029
Size: 802 227
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 447 1029
Size: 447 582
HaeII r_GCGC'y
Cuts at: 0 190 1029
Size: 190 839
MslI CAynn'nnrTG
Cuts at: 0 434 518 1029
Size: 434 84 511
Fragments arranged by size:
511 434 84
NcoI C'CATG_G
Cuts at: 0 977 1029
Size: 977 52
PfoI T'CCnGG_A
Cuts at: 0 851 1029
Size: 851 178
PspXI vC'TCGA_Gb
Cuts at: 0 5 1029
Size: 5 1024
PstI C_TGCA'G
Cuts at: 0 957 1029
Size: 957 72
SacI G_AGCT'C
Cuts at: 0 825 1029
Size: 825 204
SfcI C'TryA_G
Cuts at: 0 953 1029
Size: 953 76
SmlI C'TyrA_G
Cuts at: 0 5 285 445 1029
Size: 5 280 160 584
Fragments arranged by size:
584 280 160 5
StyI C'CwwG_G
Cuts at: 0 977 1029
Size: 977 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 601 667 1029
Size: 601 66 362
Fragments arranged by size:
601 362 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 161 193 1029
Size: 161 32 836
Fragments arranged by size:
836 161 32
XhoI C'TCGA_G
Cuts at: 0 5 1029
Size: 5 1024
XmnI GAAnn'nnTTC
Cuts at: 0 188 578 700 1029
Size: 188 390 122 329
Fragments arranged by size:
390 329 188 122
Enzymes that do cut:
AcuI AfeI AlfI AlwNI ApoI AvaI BamHI BanI
BanII BbvCI BglI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsgI BsiHKAI Bsp1286I BspHI BstAPI BstXI
BstYI BtgI DraI DraIII EarI EcoICRI Eco57MI EcoRI
FalI HaeII MslI NcoI PfoI PspXI PstI SacI
SfcI SmlI StyI TaqII TstI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI BmtI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiWI BsmI BsmBI BspEI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DrdI
EaeI EagI EciI EcoNI EcoO109I EcoRV FseI FspI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
Tth111I XbaI XcmI XmaI ZraI