(Linear) (Six-base) MAPSORT of: KIBB9833.seq  Check: 7772  from: 1  to: 1035



KIBB9833.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AccI GT'mk_AC

Cuts at:      0     171    1035 
   Size:        171     864
 
AflII C'TTAA_G

Cuts at:      0     106    1035 
   Size:        106     929
 
AflIII A'CryG_T

Cuts at:      0      69    1035 
   Size:         69     966
 
ApoI r'AATT_y

Cuts at:      0      27     817     975    1035 
   Size:         27     790     158      60

  Fragments arranged by size: 

                 790     158      60      27
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     143    1035 
   Size:        143     892
 
Bme1580I G_kGCm'C

Cuts at:      0     128    1035 
   Size:        128     907
 
BmgBI CAC'GTC

Cuts at:      0      70    1035 
   Size:         70     965
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     190    1035 
   Size:        190     845
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     886    1035 
   Size:        886     149
 
BsaAI yAC'GTr

Cuts at:      0     339     607    1035 
   Size:        339     268     428

  Fragments arranged by size: 

                 428     339     268
 
BseYI C'CCAG_C

Cuts at:      0     432     740    1035 
   Size:        432     308     295

  Fragments arranged by size: 

                 432     308     295
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      60    1035 
   Size:         60     975
 
Bsp1286I G_dGCh'C

Cuts at:      0     128    1035 
   Size:        128     907
 
BsrGI T'GTAC_A

Cuts at:      0      47    1035 
   Size:         47     988
 
BstYI r'GATC_y

Cuts at:      0     264    1035 
   Size:        264     771
 
BstZ17I GTA'TAC

Cuts at:      0     172    1035 
   Size:        172     863
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     443    1035 
   Size:        443     592
 
BtsI GCAGTG_nn'

Cuts at:      0     533    1035 
   Size:        533     502
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     204     239    1035 
   Size:        204      35     796

  Fragments arranged by size: 

                 796     204      35
 
EaeI y'GGCC_r

Cuts at:      0     585    1035 
   Size:        585     450
 
EarI CTCTTCn'nnn_

Cuts at:      0     672    1035 
   Size:        672     363
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     886    1035 
   Size:        886     149
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     421     453    1035 
   Size:        421      32     582

  Fragments arranged by size: 

                 582     421      32
 
HincII GTy'rAC

Cuts at:      0      75    1035 
   Size:         75     960
 
HindIII A'AGCT_T

Cuts at:      0      55    1035 
   Size:         55     980
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     350    1035 
   Size:        350     685
 
NspI r_CATG'y

Cuts at:      0     996    1035 
   Size:        996      39
 
SmlI C'TyrA_G

Cuts at:      0     106    1035 
   Size:        106     929
 
StuI AGG'CCT

Cuts at:      0     944    1035 
   Size:        944      91
 
StyI C'CwwG_G

Cuts at:      0     812    1035 
   Size:        812     223
 
TatI w'GTAC_w

Cuts at:      0      47     905    1035 
   Size:         47     858     130

  Fragments arranged by size: 

                 858     130      47
 
XmnI GAAnn'nnTTC

Cuts at:      0     101    1035 
   Size:        101     934
 

 Enzymes that do cut:

     AccI    AflII   AflIII     ApoI    BciVI Bme1580I    BmgBI     BmrI
     BpmI    BsaAI    BseYI    BsmBI Bsp1286I    BsrGI    BstYI  BstZ17I
    BtgZI     BtsI    CspCI     EaeI     EarI  Eco57MI    Hin4I   HincII
  HindIII     MmeI     NspI     SmlI     StuI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI   Bsu36I     BtgI     ClaI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI