(Linear) (Six-base) MAPSORT of: KIBB9837.seq Check: 6455 from: 1 to: 1056 KIBB9837.orf With 182 enzymes: SgfI * March 3, 2008 11:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 538 828 1056 Size: 538 290 228 Fragments arranged by size: 538 290 228 Acc65I G'GTAC_C Cuts at: 0 377 1056 Size: 377 679 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 1056 Size: 202 854 AleI CACnn'nnGTG Cuts at: 0 457 1056 Size: 457 599 ApoI r'AATT_y Cuts at: 0 573 784 1056 Size: 573 211 272 Fragments arranged by size: 573 272 211 AvaI C'yCGr_G Cuts at: 0 642 994 1056 Size: 642 352 62 Fragments arranged by size: 642 352 62 BanI G'GyrC_C Cuts at: 0 377 1056 Size: 377 679 BanII G_rGCy'C Cuts at: 0 56 261 320 747 1056 Size: 56 205 59 427 309 Fragments arranged by size: 427 309 205 59 56 BbsI GAAGACnn'nnnn_ Cuts at: 0 511 921 1056 Size: 511 410 135 Fragments arranged by size: 511 410 135 BclI T'GATC_A Cuts at: 0 449 1056 Size: 449 607 BglII A'GATC_T Cuts at: 0 502 1056 Size: 502 554 BmrI ACTGGGnnnn_n' Cuts at: 0 270 1037 1056 Size: 270 767 19 Fragments arranged by size: 767 270 19 BsaBI GATnn'nnATC Cuts at: 0 595 1056 Size: 595 461 BsmBI CGTCTCn'nnnn_ Cuts at: 0 482 1056 Size: 482 574 Bsp1286I G_dGCh'C Cuts at: 0 56 261 320 747 1056 Size: 56 205 59 427 309 Fragments arranged by size: 427 309 205 59 56 BspHI T'CATG_A Cuts at: 0 719 1056 Size: 719 337 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 212 538 828 1056 Size: 212 326 290 228 Fragments arranged by size: 326 290 228 212 BsrBI CCG'CTC Cuts at: 0 105 1056 Size: 105 951 BsrDI GCAATG_nn' Cuts at: 0 944 1056 Size: 944 112 BstEII G'GTnAC_C Cuts at: 0 423 1056 Size: 423 633 BstYI r'GATC_y Cuts at: 0 502 1056 Size: 502 554 BtsI GCAGTG_nn' Cuts at: 0 864 1056 Size: 864 192 ClaI AT'CG_AT Cuts at: 0 371 1056 Size: 371 685 DraI TTT'AAA Cuts at: 0 410 1056 Size: 410 646 EaeI y'GGCC_r Cuts at: 0 215 1056 Size: 215 841 EarI CTCTTCn'nnn_ Cuts at: 0 178 679 742 1056 Size: 178 501 63 314 Fragments arranged by size: 501 314 178 63 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 1056 Size: 202 854 EcoRI G'AATT_C Cuts at: 0 784 1056 Size: 784 272 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 535 1056 Size: 535 521 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 834 866 1056 Size: 834 32 190 Fragments arranged by size: 834 190 32 HincII GTy'rAC Cuts at: 0 304 1056 Size: 304 752 KpnI G_GTAC'C Cuts at: 0 381 1056 Size: 381 675 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 803 1056 Size: 803 253 MscI TGG'CCA Cuts at: 0 217 1056 Size: 217 839 MslI CAynn'nnrTG Cuts at: 0 457 718 1056 Size: 457 261 338 Fragments arranged by size: 457 338 261 MspA1I CmG'CkG Cuts at: 0 618 1056 Size: 618 438 NspI r_CATG'y Cuts at: 0 147 1056 Size: 147 909 PfoI T'CCnGG_A Cuts at: 0 779 1056 Size: 779 277 PstI C_TGCA'G Cuts at: 0 535 1056 Size: 535 521 SapI GCTCTTCn'nnn_ Cuts at: 0 178 1056 Size: 178 878 SfcI C'TryA_G Cuts at: 0 531 1056 Size: 531 525 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 447 1056 Size: 447 609 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 283 787 1056 Size: 283 504 269 Fragments arranged by size: 504 283 269 XmnI GAAnn'nnTTC Cuts at: 0 518 574 775 1056 Size: 518 56 201 281 Fragments arranged by size: 518 281 201 56 Enzymes that do cut: AarI Acc65I AcuI AleI ApoI AvaI BanI BanII BbsI BclI BglII BmrI BsaBI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BstEII BstYI BtsI ClaI DraI EaeI EarI Eco57MI EcoRI FalI Hin4I HincII KpnI MmeI MscI MslI MspA1I NspI PfoI PstI SapI SfcI TaqII XcmI XmnI Enzymes that do not cut: AatII AccI AclI AfeI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BspEI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgI BtgZI CspCI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TatI TstI Tth111I XbaI XhoI XmaI ZraI