Restriction map for
pF1KB9842
(Linear) (Six-base) MAPSORT of: KIBB9842.seq Check: 7683 from: 1 to: 1080
KIBB9842.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AccI GT'mk_AC
Cuts at: 0 254 560 1080
Size: 254 306 520
Fragments arranged by size:
520 306 254
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 1040 1080
Size: 394 646 40
Fragments arranged by size:
646 394 40
AfeI AGC'GCT
Cuts at: 0 475 1080
Size: 475 605
AlwNI CAG_nnn'CTG
Cuts at: 0 506 1080
Size: 506 574
ApoI r'AATT_y
Cuts at: 0 487 1080
Size: 487 593
BamHI G'GATC_C
Cuts at: 0 635 1080
Size: 635 445
BanII G_rGCy'C
Cuts at: 0 885 1080
Size: 885 195
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 855 889 1080
Size: 855 34 191
Fragments arranged by size:
855 191 34
BclI T'GATC_A
Cuts at: 0 674 1001 1080
Size: 674 327 79
Fragments arranged by size:
674 327 79
BglII A'GATC_T
Cuts at: 0 54 1080
Size: 54 1026
BlpI GC'TnA_GC
Cuts at: 0 181 1080
Size: 181 899
Bme1580I G_kGCm'C
Cuts at: 0 1064 1080
Size: 1064 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 639 1080
Size: 639 441
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 961 1080
Size: 961 119
BsaI GGTCTCn'nnnn_
Cuts at: 0 369 844 1080
Size: 369 475 236
Fragments arranged by size:
475 369 236
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 964 1080
Size: 964 116
BseYI C'CCAG_C
Cuts at: 0 189 579 1080
Size: 189 390 501
Fragments arranged by size:
501 390 189
BsiEI CG_ry'CG
Cuts at: 0 237 879 1080
Size: 237 642 201
Fragments arranged by size:
642 237 201
BsiWI C'GTAC_G
Cuts at: 0 706 1080
Size: 706 374
Bsp1286I G_dGCh'C
Cuts at: 0 885 1064 1080
Size: 885 179 16
Fragments arranged by size:
885 179 16
BspHI T'CATG_A
Cuts at: 0 827 1080
Size: 827 253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 266 1080
Size: 266 814
BsrBI CCG'CTC
Cuts at: 0 239 1080
Size: 239 841
BssSI C'ACGA_G
Cuts at: 0 202 1080
Size: 202 878
BstXI CCAn_nnnn'nTGG
Cuts at: 0 652 1080
Size: 652 428
BstYI r'GATC_y
Cuts at: 0 54 635 1080
Size: 54 581 445
Fragments arranged by size:
581 445 54
BtgI C'CryG_G
Cuts at: 0 966 1080
Size: 966 114
BtsI GCAGTG_nn'
Cuts at: 0 667 1080
Size: 667 413
EaeI y'GGCC_r
Cuts at: 0 234 275 437 951 1080
Size: 234 41 162 514 129
Fragments arranged by size:
514 234 162 129 41
EagI C'GGCC_G
Cuts at: 0 234 1080
Size: 234 846
EarI CTCTTCn'nnn_
Cuts at: 0 1056 1080
Size: 1056 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 636 1080
Size: 636 444
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 1040 1080
Size: 394 646 40
Fragments arranged by size:
646 394 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 355 1080
Size: 355 725
EcoO109I rG'GnC_Cy
Cuts at: 0 833 1080
Size: 833 247
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 495 1080
Size: 495 585
HaeII r_GCGC'y
Cuts at: 0 477 864 1080
Size: 477 387 216
Fragments arranged by size:
477 387 216
HincII GTy'rAC
Cuts at: 0 384 1080
Size: 384 696
MscI TGG'CCA
Cuts at: 0 439 953 1080
Size: 439 514 127
Fragments arranged by size:
514 439 127
MslI CAynn'nnrTG
Cuts at: 0 351 421 826 1080
Size: 351 70 405 254
Fragments arranged by size:
405 351 254 70
MspA1I CmG'CkG
Cuts at: 0 920 1080
Size: 920 160
NcoI C'CATG_G
Cuts at: 0 966 1080
Size: 966 114
NdeI CA'TA_TG
Cuts at: 0 493 1080
Size: 493 587
PfoI T'CCnGG_A
Cuts at: 0 845 1080
Size: 845 235
PpuMI rG'GwC_Cy
Cuts at: 0 833 1080
Size: 833 247
PvuII CAG'CTG
Cuts at: 0 920 1080
Size: 920 160
SapI GCTCTTCn'nnn_
Cuts at: 0 1056 1080
Size: 1056 24
SexAI A'CCwGG_T
Cuts at: 0 803 1080
Size: 803 277
SfcI C'TryA_G
Cuts at: 0 390 1080
Size: 390 690
SmlI C'TyrA_G
Cuts at: 0 976 1080
Size: 976 104
StuI AGG'CCT
Cuts at: 0 1035 1080
Size: 1035 45
StyI C'CwwG_G
Cuts at: 0 272 320 966 1080
Size: 272 48 646 114
Fragments arranged by size:
646 272 114 48
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 900 1080
Size: 900 180
Enzymes that do cut:
AccI AcuI AfeI AlwNI ApoI BamHI BanII BcgI
BclI BglII BlpI Bme1580I BmrI BpuEI BsaI BseRI
BseYI BsiEI BsiWI Bsp1286I BspHI BspMI BsrBI BssSI
BstXI BstYI BtgI BtsI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I FalI HaeII HincII MscI MslI
MspA1I NcoI NdeI PfoI PpuMI PvuII SapI SexAI
SfcI SmlI StuI StyI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BanI BbeI BbsI BbvCI BciVI
BfrBI BglI BmgBI BmtI BplI BpmI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsaXI BsgI BsiHKAI BsmI BsmBI
BspEI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EcoICRI EcoRI EcoRV FseI FspI FspAI Hin4I HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI