Restriction map for
pF1KB9845
(Linear) (Six-base) MAPSORT of: KIBB9845.seq Check: 1114 from: 1 to: 1119
KIBB9845.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AccI GT'mk_AC
Cuts at: 0 170 1119
Size: 170 949
Acc65I G'GTAC_C
Cuts at: 0 571 1119
Size: 571 548
AflIII A'CryG_T
Cuts at: 0 266 1119
Size: 266 853
AgeI A'CCGG_T
Cuts at: 0 443 1119
Size: 443 676
AhdI GACnn_n'nnGTC
Cuts at: 0 929 1119
Size: 929 190
AleI CACnn'nnGTG
Cuts at: 0 664 1119
Size: 664 455
ApaI G_GGCC'C
Cuts at: 0 858 1119
Size: 858 261
ApaLI G'TGCA_C
Cuts at: 0 657 1119
Size: 657 462
ApoI r'AATT_y
Cuts at: 0 1044 1119
Size: 1044 75
AseI AT'TA_AT
Cuts at: 0 715 1119
Size: 715 404
AvaI C'yCGr_G
Cuts at: 0 1007 1119
Size: 1007 112
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 438 471 555 563 588 596 1119
Size: 438 33 84 8 25 8 523
Fragments arranged by size:
523 438 84 33 25 8 8
BanI G'GyrC_C
Cuts at: 0 571 1119
Size: 571 548
BanII G_rGCy'C
Cuts at: 0 858 1119
Size: 858 261
BbsI GAAGACnn'nnnn_
Cuts at: 0 222 1070 1119
Size: 222 848 49
Fragments arranged by size:
848 222 49
BfrBI ATG'CAT
Cuts at: 0 431 1119
Size: 431 688
BglI GCCn_nnn'nGGC
Cuts at: 0 52 1119
Size: 52 1067
BglII A'GATC_T
Cuts at: 0 741 1119
Size: 741 378
Bme1580I G_kGCm'C
Cuts at: 0 441 661 858 1119
Size: 441 220 197 261
Fragments arranged by size:
441 261 220 197
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 1119
Size: 267 852
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 870 1119
Size: 454 416 249
Fragments arranged by size:
454 416 249
BsaAI yAC'GTr
Cuts at: 0 300 914 1119
Size: 300 614 205
Fragments arranged by size:
614 300 205
BsaBI GATnn'nnATC
Cuts at: 0 508 1119
Size: 508 611
BsaWI w'CCGG_w
Cuts at: 0 443 1119
Size: 443 676
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 99 746 777 1119
Size: 99 647 31 342
Fragments arranged by size:
647 342 99 31
BseYI C'CCAG_C
Cuts at: 0 876 1119
Size: 876 243
BsiEI CG_ry'CG
Cuts at: 0 89 665 1119
Size: 89 576 454
Fragments arranged by size:
576 454 89
BsiHKAI G_wGCw'C
Cuts at: 0 661 1119
Size: 661 458
BsmI GAATG_Cn'
Cuts at: 0 504 1119
Size: 504 615
BsmBI CGTCTCn'nnnn_
Cuts at: 0 111 1119
Size: 111 1008
Bsp1286I G_dGCh'C
Cuts at: 0 441 661 858 1119
Size: 441 220 197 261
Fragments arranged by size:
441 261 220 197
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 167 638 1119
Size: 167 471 481
Fragments arranged by size:
481 471 167
BsrBI CCG'CTC
Cuts at: 0 251 1119
Size: 251 868
BsrDI GCAATG_nn'
Cuts at: 0 887 1119
Size: 887 232
BsrFI r'CCGG_y
Cuts at: 0 18 55 162 443 482 1119
Size: 18 37 107 281 39 637
Fragments arranged by size:
637 281 107 39 37 18
BsrGI T'GTAC_A
Cuts at: 0 263 1119
Size: 263 856
BstAPI GCAn_nnn'nTGC
Cuts at: 0 886 1119
Size: 886 233
BstYI r'GATC_y
Cuts at: 0 350 741 1119
Size: 350 391 378
Fragments arranged by size:
391 378 350
BtgI C'CryG_G
Cuts at: 0 665 1119
Size: 665 454
BtsI GCAGTG_nn'
Cuts at: 0 95 1119
Size: 95 1024
ClaI AT'CG_AT
Cuts at: 0 772 1119
Size: 772 347
EaeI y'GGCC_r
Cuts at: 0 16 53 212 284 668 1119
Size: 16 37 159 72 384 451
Fragments arranged by size:
451 384 159 72 37 16
EarI CTCTTCn'nnn_
Cuts at: 0 565 1119
Size: 565 554
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 1119
Size: 267 852
EcoO109I rG'GnC_Cy
Cuts at: 0 854 1119
Size: 854 265
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 159 1040 1119
Size: 159 881 79
Fragments arranged by size:
881 159 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 601 633 915 947 1119
Size: 601 32 282 32 172
Fragments arranged by size:
601 282 172 32 32
HindIII A'AGCT_T
Cuts at: 0 1022 1119
Size: 1022 97
KpnI G_GTAC'C
Cuts at: 0 575 1119
Size: 575 544
MscI TGG'CCA
Cuts at: 0 214 670 1119
Size: 214 456 449
Fragments arranged by size:
456 449 214
MslI CAynn'nnrTG
Cuts at: 0 220 259 535 664 1119
Size: 220 39 276 129 455
Fragments arranged by size:
455 276 220 129 39
MspA1I CmG'CkG
Cuts at: 0 452 1119
Size: 452 667
NaeI GCC'GGC
Cuts at: 0 20 164 484 1119
Size: 20 144 320 635
Fragments arranged by size:
635 320 144 20
NgoMIV G'CCGG_C
Cuts at: 0 18 162 482 1119
Size: 18 144 320 637
Fragments arranged by size:
637 320 144 18
NsiI A_TGCA'T
Cuts at: 0 433 1119
Size: 433 686
NspI r_CATG'y
Cuts at: 0 270 431 657 1119
Size: 270 161 226 462
Fragments arranged by size:
462 270 226 161
PasI CC'CwG_GG
Cuts at: 0 477 1119
Size: 477 642
PciI A'CATG_T
Cuts at: 0 266 1119
Size: 266 853
PspOMI G'GGCC_C
Cuts at: 0 854 1119
Size: 854 265
PstI C_TGCA'G
Cuts at: 0 380 1119
Size: 380 739
RsrII CG'GwC_CG
Cuts at: 0 58 1119
Size: 58 1061
SexAI A'CCwGG_T
Cuts at: 0 779 1119
Size: 779 340
SfcI C'TryA_G
Cuts at: 0 376 648 1119
Size: 376 272 471
Fragments arranged by size:
471 376 272
SmlI C'TyrA_G
Cuts at: 0 469 849 1119
Size: 469 380 270
Fragments arranged by size:
469 380 270
SspI AAT'ATT
Cuts at: 0 1080 1119
Size: 1080 39
StyI C'CwwG_G
Cuts at: 0 686 1119
Size: 686 433
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 19 1119
Size: 19 1100
TatI w'GTAC_w
Cuts at: 0 263 792 1119
Size: 263 529 327
Fragments arranged by size:
529 327 263
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 352 384 704 736 1119
Size: 352 32 320 32 383
Fragments arranged by size:
383 352 320 32 32
XbaI T'CTAG_A
Cuts at: 0 1027 1119
Size: 1027 92
Enzymes that do cut:
AccI Acc65I AflIII AgeI AhdI AleI ApaI ApaLI
ApoI AseI AvaI BaeI BanI BanII BbsI BfrBI
BglI BglII Bme1580I BpmI BpuEI BsaAI BsaBI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BstAPI BstYI BtgI BtsI
ClaI EaeI EarI Eco57MI EcoO109I FalI Hin4I HindIII
KpnI MscI MslI MspA1I NaeI NgoMIV NsiI NspI
PasI PciI PspOMI PstI RsrII SexAI SfcI SmlI
SspI StyI TaqII TatI TstI XbaI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AlfI AloI
AlwNI AscI AsiSI AvrII BamHI BbeI BbvCI BcgI
BciVI BclI BlpI BmgBI BmrI BmtI BplI Bpu10I
BsaI BsaHI BsaXI BsgI BsiWI BspEI BspHI BssHII
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HincII HpaI KasI
MfeI MluI MmeI NarI NcoI NdeI NheI NotI
NruI PacI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
Tth111I XcmI XhoI XmaI XmnI ZraI