Restriction map for
pF1KB9847
(Linear) (Six-base) MAPSORT of: KIBB9847.seq Check: 9989 from: 1 to: 1125
KIBB9847.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 952 1125
Size: 952 173
AccI GT'mk_AC
Cuts at: 0 299 1125
Size: 299 826
AlwNI CAG_nnn'CTG
Cuts at: 0 288 1125
Size: 288 837
BanI G'GyrC_C
Cuts at: 0 73 716 751 869 1125
Size: 73 643 35 118 256
Fragments arranged by size:
643 256 118 73 35
BbeI G_GCGC'C
Cuts at: 0 720 1125
Size: 720 405
BbsI GAAGACnn'nnnn_
Cuts at: 0 117 540 869 1125
Size: 117 423 329 256
Fragments arranged by size:
423 329 256 117
BglI GCCn_nnn'nGGC
Cuts at: 0 247 1125
Size: 247 878
Bme1580I G_kGCm'C
Cuts at: 0 76 754 776 1125
Size: 76 678 22 349
Fragments arranged by size:
678 349 76 22
BmgBI CAC'GTC
Cuts at: 0 117 1125
Size: 117 1008
BmrI ACTGGGnnnn_n'
Cuts at: 0 688 1125
Size: 688 437
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 352 1125
Size: 352 773
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 559 595 1057 1125
Size: 430 129 36 462 68
Fragments arranged by size:
462 430 129 68 36
BsaI GGTCTCn'nnnn_
Cuts at: 0 325 384 1125
Size: 325 59 741
Fragments arranged by size:
741 325 59
BsaHI Gr'CG_yC
Cuts at: 0 717 1125
Size: 717 408
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 265 646 1054 1125
Size: 265 381 408 71
Fragments arranged by size:
408 381 265 71
BseYI C'CCAG_C
Cuts at: 0 451 769 1125
Size: 451 318 356
Fragments arranged by size:
451 356 318
BsiHKAI G_wGCw'C
Cuts at: 0 485 830 1125
Size: 485 345 295
Fragments arranged by size:
485 345 295
Bsp1286I G_dGCh'C
Cuts at: 0 76 485 754 776 830 1125
Size: 76 409 269 22 54 295
Fragments arranged by size:
409 295 269 76 54 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 239 774 952 1100 1125
Size: 239 535 178 148 25
Fragments arranged by size:
535 239 178 148 25
BsrDI GCAATG_nn'
Cuts at: 0 96 494 1125
Size: 96 398 631
Fragments arranged by size:
631 398 96
BsrFI r'CCGG_y
Cuts at: 0 713 1125
Size: 713 412
BssSI C'ACGA_G
Cuts at: 0 400 1125
Size: 400 725
BstEII G'GTnAC_C
Cuts at: 0 172 1125
Size: 172 953
BstXI CCAn_nnnn'nTGG
Cuts at: 0 443 573 1125
Size: 443 130 552
Fragments arranged by size:
552 443 130
BstYI r'GATC_y
Cuts at: 0 320 1125
Size: 320 805
Bsu36I CC'TnA_GG
Cuts at: 0 1096 1125
Size: 1096 29
BtgI C'CryG_G
Cuts at: 0 110 894 932 1125
Size: 110 784 38 193
Fragments arranged by size:
784 193 110 38
DraIII CAC_nnn'GTG
Cuts at: 0 49 1125
Size: 49 1076
DrdI GACnn_nn'nnGTC
Cuts at: 0 121 1125
Size: 121 1004
EaeI y'GGCC_r
Cuts at: 0 524 613 1125
Size: 524 89 512
Fragments arranged by size:
524 512 89
EarI CTCTTCn'nnn_
Cuts at: 0 664 1125
Size: 664 461
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 601 1125
Size: 601 524
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 559 595 1057 1125
Size: 430 129 36 462 68
Fragments arranged by size:
462 430 129 68 36
EcoO109I rG'GnC_Cy
Cuts at: 0 165 1002 1091 1125
Size: 165 837 89 34
Fragments arranged by size:
837 165 89 34
HaeII r_GCGC'y
Cuts at: 0 720 1031 1125
Size: 720 311 94
Fragments arranged by size:
720 311 94
HincII GTy'rAC
Cuts at: 0 951 1125
Size: 951 174
KasI G'GCGC_C
Cuts at: 0 716 1125
Size: 716 409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1025 1125
Size: 1025 100
MscI TGG'CCA
Cuts at: 0 526 615 1125
Size: 526 89 510
Fragments arranged by size:
526 510 89
MslI CAynn'nnrTG
Cuts at: 0 307 370 1125
Size: 307 63 755
Fragments arranged by size:
755 307 63
MspA1I CmG'CkG
Cuts at: 0 288 1125
Size: 288 837
NarI GG'CG_CC
Cuts at: 0 717 1125
Size: 717 408
NcoI C'CATG_G
Cuts at: 0 932 1125
Size: 932 193
NspI r_CATG'y
Cuts at: 0 369 847 1125
Size: 369 478 278
Fragments arranged by size:
478 369 278
PasI CC'CwG_GG
Cuts at: 0 69 1125
Size: 69 1056
PfoI T'CCnGG_A
Cuts at: 0 536 1125
Size: 536 589
PpuMI rG'GwC_Cy
Cuts at: 0 165 1091 1125
Size: 165 926 34
Fragments arranged by size:
926 165 34
PshAI GACnn'nnGTC
Cuts at: 0 295 1125
Size: 295 830
PstI C_TGCA'G
Cuts at: 0 725 1125
Size: 725 400
SanDI GG'GwC_CC
Cuts at: 0 1091 1125
Size: 1091 34
ScaI AGT'ACT
Cuts at: 0 667 1125
Size: 667 458
SfcI C'TryA_G
Cuts at: 0 141 721 756 1125
Size: 141 580 35 369
Fragments arranged by size:
580 369 141 35
SfoI GGC'GCC
Cuts at: 0 718 1125
Size: 718 407
StuI AGG'CCT
Cuts at: 0 466 1125
Size: 466 659
StyI C'CwwG_G
Cuts at: 0 566 932 1125
Size: 566 366 193
Fragments arranged by size:
566 366 193
TatI w'GTAC_w
Cuts at: 0 665 1068 1125
Size: 665 403 57
Fragments arranged by size:
665 403 57
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 534 1125
Size: 534 591
XmnI GAAnn'nnTTC
Cuts at: 0 547 1125
Size: 547 578
Enzymes that do cut:
AarI AccI AlwNI BanI BbeI BbsI BglI Bme1580I
BmgBI BmrI BplI BpmI BsaI BsaHI BseRI BseYI
BsiHKAI Bsp1286I BspMI BsrDI BsrFI BssSI BstEII BstXI
BstYI Bsu36I BtgI DraIII DrdI EaeI EarI EciI
Eco57MI EcoO109I HaeII HincII KasI MmeI MscI MslI
MspA1I NarI NcoI NspI PasI PfoI PpuMI PshAI
PstI SanDI ScaI SfcI SfoI StuI StyI TatI
XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanII BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmtI Bpu10I
BpuEI BsaAI BsaBI BsaWI BsaXI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII BstAPI
BstBI BstZ17I BtgZI BtsI ClaI CspCI DraI EagI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HindIII HpaI KpnI MfeI MluI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PflMI
PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI SexAI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TstI Tth111I XbaI XhoI
XmaI ZraI