Restriction map for
pF1KB9850
(Linear) (Six-base) MAPSORT of: KIBB9850.seq Check: 8155 from: 1 to: 1131
KIBB9850.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AfeI AGC'GCT
Cuts at: 0 712 1131
Size: 712 419
AlwNI CAG_nnn'CTG
Cuts at: 0 410 742 1131
Size: 410 332 389
Fragments arranged by size:
410 389 332
AvaI C'yCGr_G
Cuts at: 0 881 1131
Size: 881 250
BamHI G'GATC_C
Cuts at: 0 87 995 1131
Size: 87 908 136
Fragments arranged by size:
908 136 87
BglII A'GATC_T
Cuts at: 0 113 581 1131
Size: 113 468 550
Fragments arranged by size:
550 468 113
Bme1580I G_kGCm'C
Cuts at: 0 791 855 1131
Size: 791 64 276
Fragments arranged by size:
791 276 64
BmrI ACTGGGnnnn_n'
Cuts at: 0 420 1131
Size: 420 711
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 807 1131
Size: 807 324
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 756 1131
Size: 85 671 375
Fragments arranged by size:
671 375 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 1131
Size: 94 1037
BsaAI yAC'GTr
Cuts at: 0 359 828 1131
Size: 359 469 303
Fragments arranged by size:
469 359 303
BsaWI w'CCGG_w
Cuts at: 0 1118 1131
Size: 1118 13
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 538 568 1131
Size: 538 30 563
Fragments arranged by size:
563 538 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 562 1131
Size: 562 569
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 348 1131
Size: 348 783
BsmI GAATG_Cn'
Cuts at: 0 66 1131
Size: 66 1065
Bsp1286I G_dGCh'C
Cuts at: 0 791 855 1131
Size: 791 64 276
Fragments arranged by size:
791 276 64
BspEI T'CCGG_A
Cuts at: 0 1118 1131
Size: 1118 13
BsrBI CCG'CTC
Cuts at: 0 519 1131
Size: 519 612
BsrDI GCAATG_nn'
Cuts at: 0 392 1131
Size: 392 739
BstYI r'GATC_y
Cuts at: 0 52 87 113 581 656 995 1131
Size: 52 35 26 468 75 339 136
Fragments arranged by size:
468 339 136 75 52 35 26
BtgI C'CryG_G
Cuts at: 0 125 719 1131
Size: 125 594 412
Fragments arranged by size:
594 412 125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 270 1131
Size: 270 861
DraI TTT'AAA
Cuts at: 0 1040 1131
Size: 1040 91
EaeI y'GGCC_r
Cuts at: 0 149 239 318 456 464 651 1131
Size: 149 90 79 138 8 187 480
Fragments arranged by size:
480 187 149 138 90 79 8
EarI CTCTTCn'nnn_
Cuts at: 0 526 1076 1131
Size: 526 550 55
Fragments arranged by size:
550 526 55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 756 1131
Size: 85 671 375
Fragments arranged by size:
671 375 85
EcoNI CCTnn'n_nnAGG
Cuts at: 0 34 781 1131
Size: 34 747 350
Fragments arranged by size:
747 350 34
EcoO109I rG'GnC_Cy
Cuts at: 0 95 611 749 923 1131
Size: 95 516 138 174 208
Fragments arranged by size:
516 208 174 138 95
HaeII r_GCGC'y
Cuts at: 0 109 714 1009 1131
Size: 109 605 295 122
Fragments arranged by size:
605 295 122 109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 538 570 1131
Size: 538 32 561
Fragments arranged by size:
561 538 32
HindIII A'AGCT_T
Cuts at: 0 838 1094 1131
Size: 838 256 37
Fragments arranged by size:
838 256 37
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 536 1131
Size: 71 465 595
Fragments arranged by size:
595 465 71
MscI TGG'CCA
Cuts at: 0 151 241 320 458 1131
Size: 151 90 79 138 673
Fragments arranged by size:
673 151 138 90 79
MslI CAynn'nnrTG
Cuts at: 0 386 1131
Size: 386 745
PflMI CCAn_nnn'nTGG
Cuts at: 0 485 1131
Size: 485 646
PmlI CAC'GTG
Cuts at: 0 359 828 1131
Size: 359 469 303
Fragments arranged by size:
469 359 303
PpuMI rG'GwC_Cy
Cuts at: 0 95 749 1131
Size: 95 654 382
Fragments arranged by size:
654 382 95
SapI GCTCTTCn'nnn_
Cuts at: 0 526 1131
Size: 526 605
SmlI C'TyrA_G
Cuts at: 0 109 881 1131
Size: 109 772 250
Fragments arranged by size:
772 250 109
StuI AGG'CCT
Cuts at: 0 29 43 1126 1131
Size: 29 14 1083 5
Fragments arranged by size:
1083 29 14 5
StyI C'CwwG_G
Cuts at: 0 536 1131
Size: 536 595
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 316 1131
Size: 316 815
TatI w'GTAC_w
Cuts at: 0 174 1131
Size: 174 957
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 252 903 1131
Size: 252 651 228
Fragments arranged by size:
651 252 228
XhoI C'TCGA_G
Cuts at: 0 881 1131
Size: 881 250
XmnI GAAnn'nnTTC
Cuts at: 0 29 712 1085 1131
Size: 29 683 373 46
Fragments arranged by size:
683 373 46 29
Enzymes that do cut:
AfeI AlwNI AvaI BamHI BglII Bme1580I BmrI BplI
BpmI BpuEI BsaAI BsaWI BsaXI BseRI BsgI BsmI
Bsp1286I BspEI BsrBI BsrDI BstYI BtgI BtgZI DraI
EaeI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII
MmeI MscI MslI PflMI PmlI PpuMI SapI SmlI
StuI StyI TaqII TatI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BlpI
BmgBI BmtI Bpu10I BsaI BsaBI BsaHI BseYI BsiEI
BsiHKAI BsiWI BsmBI BspHI BspMI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtsI
ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HincII HpaI KasI
KpnI MfeI MluI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TstI Tth111I XbaI XmaI ZraI