Restriction map for
pF1KB9858
(Linear) (Six-base) MAPSORT of: KIBB9858.seq Check: 9108 from: 1 to: 1200
KIBB9858.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1102 1200
Size: 1102 98
AhdI GACnn_n'nnGTC
Cuts at: 0 664 1200
Size: 664 536
AlwNI CAG_nnn'CTG
Cuts at: 0 775 1165 1200
Size: 775 390 35
Fragments arranged by size:
775 390 35
ApaI G_GGCC'C
Cuts at: 0 861 1200
Size: 861 339
BamHI G'GATC_C
Cuts at: 0 220 376 1200
Size: 220 156 824
Fragments arranged by size:
824 220 156
BanI G'GyrC_C
Cuts at: 0 244 487 541 830 1200
Size: 244 243 54 289 370
Fragments arranged by size:
370 289 244 243 54
BanII G_rGCy'C
Cuts at: 0 116 124 447 641 861 982 1063 1175
Size: 116 8 323 194 220 121 81 112
Cuts at: 1175 1200
Size: 25
Fragments arranged by size:
323 220 194 121 116 112 81 25
8
BbeI G_GCGC'C
Cuts at: 0 834 1200
Size: 834 366
BbvCI CC'TCA_GC
Cuts at: 0 186 1200
Size: 186 1014
BglI GCCn_nnn'nGGC
Cuts at: 0 793 1200
Size: 793 407
BlpI GC'TnA_GC
Cuts at: 0 868 1200
Size: 868 332
Bme1580I G_kGCm'C
Cuts at: 0 317 731 861 1200
Size: 317 414 130 339
Fragments arranged by size:
414 339 317 130
BmrI ACTGGGnnnn_n'
Cuts at: 0 182 602 863 1200
Size: 182 420 261 337
Fragments arranged by size:
420 337 261 182
BmtI G_CTAG'C
Cuts at: 0 467 1200
Size: 467 733
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 411 465 493 547 601 627 655
Size: 401 10 54 28 54 54 26 28
Cuts at: 655 1200
Size: 545
Fragments arranged by size:
545 401 54 54 54 28 28 26
10
Bpu10I CC'TnA_GC
Cuts at: 0 118 186 269 1200
Size: 118 68 83 931
Fragments arranged by size:
931 118 83 68
BsaI GGTCTCn'nnnn_
Cuts at: 0 105 469 577 672 1200
Size: 105 364 108 95 528
Fragments arranged by size:
528 364 108 105 95
BsaHI Gr'CG_yC
Cuts at: 0 831 902 1154 1200
Size: 831 71 252 46
Fragments arranged by size:
831 252 71 46
BsaWI w'CCGG_w
Cuts at: 0 85 734 1200
Size: 85 649 466
Fragments arranged by size:
649 466 85
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 399 429 434 464 1069 1099 1200
Size: 399 30 5 30 605 30 101
Fragments arranged by size:
605 399 101 30 30 30 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 946 1090 1129 1132 1200
Size: 946 144 39 3 68
Fragments arranged by size:
946 144 68 39 3
BseYI C'CCAG_C
Cuts at: 0 910 1099 1158 1200
Size: 910 189 59 42
Fragments arranged by size:
910 189 59 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 1200
Size: 91 1109
BsiHKAI G_wGCw'C
Cuts at: 0 61 124 1200
Size: 61 63 1076
Fragments arranged by size:
1076 63 61
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1178 1200
Size: 1178 22
Bsp1286I G_dGCh'C
Cuts at: 0 61 116 124 317 447 641 731 861
Size: 61 55 8 193 130 194 90 130
Cuts at: 861 982 1063 1175 1200
Size: 121 81 112 25
Fragments arranged by size:
194 193 130 130 121 112 90 81
61 55 25 8
BspEI T'CCGG_A
Cuts at: 0 85 734 1200
Size: 85 649 466
Fragments arranged by size:
649 466 85
BsrFI r'CCGG_y
Cuts at: 0 833 1200
Size: 833 367
BsrGI T'GTAC_A
Cuts at: 0 131 1200
Size: 131 1069
BssSI C'ACGA_G
Cuts at: 0 755 808 862 1200
Size: 755 53 54 338
Fragments arranged by size:
755 338 54 53
BstYI r'GATC_y
Cuts at: 0 220 376 1200
Size: 220 156 824
Fragments arranged by size:
824 220 156
Bsu36I CC'TnA_GG
Cuts at: 0 158 929 1200
Size: 158 771 271
Fragments arranged by size:
771 271 158
BtgI C'CryG_G
Cuts at: 0 455 509 563 953 1200
Size: 455 54 54 390 247
Fragments arranged by size:
455 390 247 54 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1084 1200
Size: 1084 116
BtsI GCAGTG_nn'
Cuts at: 0 74 1200
Size: 74 1126
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1017 1052 1200
Size: 1017 35 148
Fragments arranged by size:
1017 148 35
DraIII CAC_nnn'GTG
Cuts at: 0 387 1200
Size: 387 813
EaeI y'GGCC_r
Cuts at: 0 2 1130 1200
Size: 2 1128 70
Fragments arranged by size:
1128 70 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 864 1200
Size: 864 336
EcoICRI GAG'CTC
Cuts at: 0 122 1200
Size: 122 1078
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 411 465 493 547 601 627 655
Size: 401 10 54 28 54 54 26 28
Cuts at: 655 1102 1200
Size: 447 98
Fragments arranged by size:
447 401 98 54 54 54 28 28
26 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 429 1200
Size: 429 771
EcoO109I rG'GnC_Cy
Cuts at: 0 857 858 924 1096 1200
Size: 857 1 66 172 104
Fragments arranged by size:
857 172 104 66 1
HaeII r_GCGC'y
Cuts at: 0 834 1200
Size: 834 366
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 169 201 650 682 736 768 964 996
Size: 169 32 449 32 54 32 196 32
Cuts at: 996 1069 1101 1200
Size: 73 32 99
Fragments arranged by size:
449 196 169 99 73 54 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 830 1200
Size: 830 370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 1200
Size: 724 476
MscI TGG'CCA
Cuts at: 0 4 1132 1200
Size: 4 1128 68
Fragments arranged by size:
1128 68 4
MslI CAynn'nnrTG
Cuts at: 0 301 1200
Size: 301 899
MspA1I CmG'CkG
Cuts at: 0 647 1200
Size: 647 553
NaeI GCC'GGC
Cuts at: 0 835 1200
Size: 835 365
NarI GG'CG_CC
Cuts at: 0 831 1200
Size: 831 369
NcoI C'CATG_G
Cuts at: 0 509 563 1200
Size: 509 54 637
Fragments arranged by size:
637 509 54
NgoMIV G'CCGG_C
Cuts at: 0 833 1200
Size: 833 367
NheI G'CTAG_C
Cuts at: 0 463 1200
Size: 463 737
NspI r_CATG'y
Cuts at: 0 765 1200
Size: 765 435
PpuMI rG'GwC_Cy
Cuts at: 0 924 1200
Size: 924 276
PshAI GACnn'nnGTC
Cuts at: 0 1047 1200
Size: 1047 153
PspOMI G'GGCC_C
Cuts at: 0 857 1200
Size: 857 343
SacI G_AGCT'C
Cuts at: 0 124 1200
Size: 124 1076
SfoI GGC'GCC
Cuts at: 0 832 1200
Size: 832 368
SgrAI Cr'CCGG_yG
Cuts at: 0 833 1200
Size: 833 367
SphI G_CATG'C
Cuts at: 0 765 1200
Size: 765 435
StyI C'CwwG_G
Cuts at: 0 21 509 563 1200
Size: 21 488 54 637
Fragments arranged by size:
637 488 54 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 93 1200
Size: 93 1107
TatI w'GTAC_w
Cuts at: 0 131 1200
Size: 131 1069
Enzymes that do cut:
AcuI AhdI AlwNI ApaI BamHI BanI BanII BbeI
BbvCI BglI BlpI Bme1580I BmrI BmtI BpmI Bpu10I
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspEI BsrFI BsrGI BssSI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraIII EaeI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PpuMI PshAI PspOMI SacI SfoI SgrAI SphI StyI
TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BbsI BcgI BciVI BclI
BfrBI BglII BmgBI BplI BpuEI BsaAI BsaBI BsiEI
BsiWI BsmI BspHI BspMI BsrBI BsrDI BssHII BstAPI
BstBI BstEII BstXI BstZ17I ClaI DraI DrdI EagI
EarI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI NdeI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PsiI PspXI PsrI PstI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SmaI SmlI SnaBI SpeI SrfI SspI
StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI