Restriction map for
pF1KB9866
(Linear) (Six-base) MAPSORT of: KIBB9866.seq Check: 199 from: 1 to: 1305
KIBB9866.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 166 968 1305
Size: 166 802 337
Fragments arranged by size:
802 337 166
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 544 1305
Size: 364 180 761
Fragments arranged by size:
761 364 180
AflIII A'CryG_T
Cuts at: 0 128 1305
Size: 128 1177
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 121 842 1305
Size: 121 721 463
Fragments arranged by size:
721 463 121
ApaI G_GGCC'C
Cuts at: 0 870 1305
Size: 870 435
AvaI C'yCGr_G
Cuts at: 0 862 1171 1305
Size: 862 309 134
Fragments arranged by size:
862 309 134
BanI G'GyrC_C
Cuts at: 0 1110 1305
Size: 1110 195
BanII G_rGCy'C
Cuts at: 0 358 870 1305
Size: 358 512 435
Fragments arranged by size:
512 435 358
BbeI G_GCGC'C
Cuts at: 0 1114 1305
Size: 1114 191
BbsI GAAGACnn'nnnn_
Cuts at: 0 30 441 604 1305
Size: 30 411 163 701
Fragments arranged by size:
701 411 163 30
BbvCI CC'TCA_GC
Cuts at: 0 1097 1305
Size: 1097 208
BglI GCCn_nnn'nGGC
Cuts at: 0 713 730 991 1064 1255 1264 1305
Size: 713 17 261 73 191 9 41
Fragments arranged by size:
713 261 191 73 41 17 9
BglII A'GATC_T
Cuts at: 0 662 1305
Size: 662 643
Bme1580I G_kGCm'C
Cuts at: 0 870 1305
Size: 870 435
BmgBI CAC'GTC
Cuts at: 0 129 1305
Size: 129 1176
BmrI ACTGGGnnnn_n'
Cuts at: 0 990 1305
Size: 990 315
BmtI G_CTAG'C
Cuts at: 0 901 1305
Size: 901 404
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 567 679 1165 1305
Size: 30 537 112 486 140
Fragments arranged by size:
537 486 140 112 30
Bpu10I CC'TnA_GC
Cuts at: 0 1097 1305
Size: 1097 208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 1003 1305
Size: 673 330 302
Fragments arranged by size:
673 330 302
BsaI GGTCTCn'nnnn_
Cuts at: 0 1229 1305
Size: 1229 76
BsaAI yAC'GTr
Cuts at: 0 285 1305
Size: 285 1020
BsaHI Gr'CG_yC
Cuts at: 0 722 1111 1305
Size: 722 389 194
Fragments arranged by size:
722 389 194
BsaWI w'CCGG_w
Cuts at: 0 1287 1305
Size: 1287 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 115 145 1305
Size: 115 30 1160
Fragments arranged by size:
1160 115 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 343 346 349 1189 1192 1195 1305
Size: 343 3 3 840 3 3 110
Fragments arranged by size:
840 343 110 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 108 167 358 738 769 1054 1305
Size: 108 59 191 380 31 285 251
Fragments arranged by size:
380 285 251 191 108 59 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 1305
Size: 274 1031
BsiEI CG_ry'CG
Cuts at: 0 405 546 781 998 1305
Size: 405 141 235 217 307
Fragments arranged by size:
405 307 235 217 141
BsiHKAI G_wGCw'C
Cuts at: 0 50 1305
Size: 50 1255
Bsp1286I G_dGCh'C
Cuts at: 0 50 358 870 1305
Size: 50 308 512 435
Fragments arranged by size:
512 435 308 50
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 166 968 1305
Size: 166 802 337
Fragments arranged by size:
802 337 166
BsrBI CCG'CTC
Cuts at: 0 531 1002 1133 1305
Size: 531 471 131 172
Fragments arranged by size:
531 471 172 131
BsrFI r'CCGG_y
Cuts at: 0 493 1305
Size: 493 812
BssHII G'CGCG_C
Cuts at: 0 913 1305
Size: 913 392
BssSI C'ACGA_G
Cuts at: 0 1090 1305
Size: 1090 215
BstAPI GCAn_nnn'nTGC
Cuts at: 0 297 1305
Size: 297 1008
BstXI CCAn_nnnn'nTGG
Cuts at: 0 567 1305
Size: 567 738
BstYI r'GATC_y
Cuts at: 0 662 1305
Size: 662 643
BtgI C'CryG_G
Cuts at: 0 53 116 405 590 851 881 1199 1305
Size: 53 63 289 185 261 30 318 106
Fragments arranged by size:
318 289 261 185 106 63 53 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 152 474 1305
Size: 152 322 831
Fragments arranged by size:
831 322 152
DraIII CAC_nnn'GTG
Cuts at: 0 297 1305
Size: 297 1008
EaeI y'GGCC_r
Cuts at: 0 422 495 778 948 995 1298 1305
Size: 422 73 283 170 47 303 7
Fragments arranged by size:
422 303 283 170 73 47 7
EagI C'GGCC_G
Cuts at: 0 778 995 1305
Size: 778 217 310
Fragments arranged by size:
778 310 217
EarI CTCTTCn'nnn_
Cuts at: 0 302 340 1305
Size: 302 38 965
Fragments arranged by size:
965 302 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 136 921 1305
Size: 136 785 384
Fragments arranged by size:
785 384 136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 364 544 567 679 1165 1305
Size: 30 334 180 23 112 486 140
Fragments arranged by size:
486 334 180 140 112 30 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 838 907 1305
Size: 838 69 398
Fragments arranged by size:
838 398 69
EcoO109I rG'GnC_Cy
Cuts at: 0 203 732 866 867 1166 1305
Size: 203 529 134 1 299 139
Fragments arranged by size:
529 299 203 139 134 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 621 1305
Size: 621 684
FspI TGC'GCA
Cuts at: 0 301 418 818 1305
Size: 301 117 400 487
Fragments arranged by size:
487 400 301 117
FspAI rTGC'GCAy
Cuts at: 0 301 418 818 1305
Size: 301 117 400 487
Fragments arranged by size:
487 400 301 117
HaeII r_GCGC'y
Cuts at: 0 238 433 1114 1157 1305
Size: 238 195 681 43 148
Fragments arranged by size:
681 238 195 148 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 162 373 405 735 767 1305
Size: 130 32 211 32 330 32 538
Fragments arranged by size:
538 330 211 130 32 32 32
KasI G'GCGC_C
Cuts at: 0 1110 1305
Size: 1110 195
MscI TGG'CCA
Cuts at: 0 1300 1305
Size: 1300 5
MslI CAynn'nnrTG
Cuts at: 0 103 250 616 1305
Size: 103 147 366 689
Fragments arranged by size:
689 366 147 103
MspA1I CmG'CkG
Cuts at: 0 118 342 407 539 548 738 826 883
Size: 118 224 65 132 9 190 88 57
Cuts at: 883 1201 1274 1305
Size: 318 73 31
Fragments arranged by size:
318 224 190 132 118 88 73 65
57 31 9
NaeI GCC'GGC
Cuts at: 0 495 1305
Size: 495 810
NarI GG'CG_CC
Cuts at: 0 1111 1305
Size: 1111 194
NgoMIV G'CCGG_C
Cuts at: 0 493 1305
Size: 493 812
NheI G'CTAG_C
Cuts at: 0 897 1305
Size: 897 408
NspI r_CATG'y
Cuts at: 0 657 1305
Size: 657 648
PasI CC'CwG_GG
Cuts at: 0 834 1305
Size: 834 471
PmlI CAC'GTG
Cuts at: 0 285 1305
Size: 285 1020
PpuMI rG'GwC_Cy
Cuts at: 0 1166 1305
Size: 1166 139
PshAI GACnn'nnGTC
Cuts at: 0 445 1241 1305
Size: 445 796 64
Fragments arranged by size:
796 445 64
PspOMI G'GGCC_C
Cuts at: 0 866 1305
Size: 866 439
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 178 210 1305
Size: 178 32 1095
Fragments arranged by size:
1095 178 32
PstI C_TGCA'G
Cuts at: 0 242 1050 1305
Size: 242 808 255
Fragments arranged by size:
808 255 242
PvuII CAG'CTG
Cuts at: 0 342 1305
Size: 342 963
SacII CC_GC'GG
Cuts at: 0 119 408 884 1202 1305
Size: 119 289 476 318 103
Fragments arranged by size:
476 318 289 119 103
SanDI GG'GwC_CC
Cuts at: 0 1166 1305
Size: 1166 139
ScaI AGT'ACT
Cuts at: 0 502 1305
Size: 502 803
SfcI C'TryA_G
Cuts at: 0 238 1046 1305
Size: 238 808 259
Fragments arranged by size:
808 259 238
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1255 1264 1305
Size: 1255 9 41
Fragments arranged by size:
1255 41 9
SfoI GGC'GCC
Cuts at: 0 1112 1305
Size: 1112 193
SmaI CCC'GGG
Cuts at: 0 864 1305
Size: 864 441
SmlI C'TyrA_G
Cuts at: 0 688 1018 1305
Size: 688 330 287
Fragments arranged by size:
688 330 287
StyI C'CwwG_G
Cuts at: 0 365 1305
Size: 365 940
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 181 922 1305
Size: 181 741 383
Fragments arranged by size:
741 383 181
TatI w'GTAC_w
Cuts at: 0 500 1305
Size: 500 805
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 115 147 1305
Size: 115 32 1158
Fragments arranged by size:
1158 115 32
XmaI C'CCGG_G
Cuts at: 0 862 1305
Size: 862 443
Enzymes that do cut:
AarI AcuI AflIII AlfI ApaI AvaI BanI BanII
BbeI BbsI BbvCI BglI BglII Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI
BtgZI DraIII EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I FalI FspI FspAI HaeII Hin4I KasI MscI
MslI MspA1I NaeI NarI NgoMIV NheI NspI PasI
PmlI PpuMI PshAI PspOMI PsrI PstI PvuII SacII
SanDI ScaI SfcI SfiI SfoI SmaI SmlI StyI
TaqII TatI TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BcgI BciVI BclI BfrBI BlpI
BplI BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrGI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI
DraI DrdI EcoICRI EcoRI EcoRV FseI HincII HindIII
HpaI KpnI MfeI MluI MmeI NcoI NdeI NotI
NruI NsiI PacI PciI PflMI PfoI PmeI PpiI
PsiI PspXI PvuI RsrII SacI SalI SapI SbfI
SexAI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XcmI XhoI XmnI ZraI