Restriction map for
pF1KB9869
(Linear) (Six-base) MAPSORT of: KIBB9869.seq Check: 5322 from: 1 to: 1380
KIBB9869.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AatII G_ACGT'C
Cuts at: 0 1067 1380
Size: 1067 313
AccI GT'mk_AC
Cuts at: 0 641 1380
Size: 641 739
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 91 1380
Size: 56 35 1289
Fragments arranged by size:
1289 56 35
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 474 841 1380
Size: 474 367 539
Fragments arranged by size:
539 474 367
AlwNI CAG_nnn'CTG
Cuts at: 0 81 493 961 1380
Size: 81 412 468 419
Fragments arranged by size:
468 419 412 81
ApaI G_GGCC'C
Cuts at: 0 805 1380
Size: 805 575
ApaLI G'TGCA_C
Cuts at: 0 220 1380
Size: 220 1160
ApoI r'AATT_y
Cuts at: 0 63 1380
Size: 63 1317
AvaI C'yCGr_G
Cuts at: 0 8 167 208 1380
Size: 8 159 41 1172
Fragments arranged by size:
1172 159 41 8
BanII G_rGCy'C
Cuts at: 0 278 805 994 1380
Size: 278 527 189 386
Fragments arranged by size:
527 386 278 189
BbvCI CC'TCA_GC
Cuts at: 0 954 1380
Size: 954 426
BglI GCCn_nnn'nGGC
Cuts at: 0 123 326 418 1380
Size: 123 203 92 962
Fragments arranged by size:
962 203 123 92
BlpI GC'TnA_GC
Cuts at: 0 101 1380
Size: 101 1279
Bme1580I G_kGCm'C
Cuts at: 0 224 551 805 1198 1380
Size: 224 327 254 393 182
Fragments arranged by size:
393 327 254 224 182
BmgBI CAC'GTC
Cuts at: 0 345 1380
Size: 345 1035
BmrI ACTGGGnnnn_n'
Cuts at: 0 1249 1380
Size: 1249 131
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1258 1380
Size: 1258 122
Bpu10I CC'TnA_GC
Cuts at: 0 954 1380
Size: 954 426
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1277 1380
Size: 1277 103
BsaHI Gr'CG_yC
Cuts at: 0 85 1064 1380
Size: 85 979 316
Fragments arranged by size:
979 316 85
BsaWI w'CCGG_w
Cuts at: 0 132 1380
Size: 132 1248
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 67 942 1012 1380
Size: 28 39 875 70 368
Fragments arranged by size:
875 368 70 39 28
BseYI C'CCAG_C
Cuts at: 0 213 693 1380
Size: 213 480 687
Fragments arranged by size:
687 480 213
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 911 1380
Size: 911 469
BsiEI CG_ry'CG
Cuts at: 0 311 422 1380
Size: 311 111 958
Fragments arranged by size:
958 311 111
BsiHKAI G_wGCw'C
Cuts at: 0 224 734 1380
Size: 224 510 646
Fragments arranged by size:
646 510 224
BsmI GAATG_Cn'
Cuts at: 0 463 1045 1380
Size: 463 582 335
Fragments arranged by size:
582 463 335
Bsp1286I G_dGCh'C
Cuts at: 0 224 278 551 734 805 994 1198 1380
Size: 224 54 273 183 71 189 204 182
Fragments arranged by size:
273 224 204 189 183 182 71 54
BsrBI CCG'CTC
Cuts at: 0 160 857 974 1380
Size: 160 697 117 406
Fragments arranged by size:
697 406 160 117
BsrFI r'CCGG_y
Cuts at: 0 191 310 1380
Size: 191 119 1070
Fragments arranged by size:
1070 191 119
BssSI C'ACGA_G
Cuts at: 0 598 1380
Size: 598 782
BstAPI GCAn_nnn'nTGC
Cuts at: 0 201 1380
Size: 201 1179
BstYI r'GATC_y
Cuts at: 0 1268 1380
Size: 1268 112
Bsu36I CC'TnA_GG
Cuts at: 0 1073 1380
Size: 1073 307
BtsI GCAGTG_nn'
Cuts at: 0 458 491 1380
Size: 458 33 889
Fragments arranged by size:
889 458 33
ClaI AT'CG_AT
Cuts at: 0 1358 1380
Size: 1358 22
EaeI y'GGCC_r
Cuts at: 0 308 399 419 1380
Size: 308 91 20 961
Fragments arranged by size:
961 308 91 20
EagI C'GGCC_G
Cuts at: 0 308 419 1380
Size: 308 111 961
Fragments arranged by size:
961 308 111
EarI CTCTTCn'nnn_
Cuts at: 0 1178 1380
Size: 1178 202
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 499 1380
Size: 499 881
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 91 1258 1380
Size: 56 35 1167 122
Fragments arranged by size:
1167 122 56 35
EcoO109I rG'GnC_Cy
Cuts at: 0 477 750 802 1332 1380
Size: 477 273 52 530 48
Fragments arranged by size:
530 477 273 52 48
EcoRI G'AATT_C
Cuts at: 0 63 1380
Size: 63 1317
MscI TGG'CCA
Cuts at: 0 401 1380
Size: 401 979
MslI CAynn'nnrTG
Cuts at: 0 1225 1380
Size: 1225 155
MspA1I CmG'CkG
Cuts at: 0 144 231 307 697 1380
Size: 144 87 76 390 683
Fragments arranged by size:
683 390 144 87 76
NaeI GCC'GGC
Cuts at: 0 193 312 1380
Size: 193 119 1068
Fragments arranged by size:
1068 193 119
NgoMIV G'CCGG_C
Cuts at: 0 191 310 1380
Size: 191 119 1070
Fragments arranged by size:
1070 191 119
NspI r_CATG'y
Cuts at: 0 578 1380
Size: 578 802
PpuMI rG'GwC_Cy
Cuts at: 0 750 1380
Size: 750 630
PshAI GACnn'nnGTC
Cuts at: 0 1091 1380
Size: 1091 289
PspOMI G'GGCC_C
Cuts at: 0 801 1380
Size: 801 579
PstI C_TGCA'G
Cuts at: 0 767 1380
Size: 767 613
PvuII CAG'CTG
Cuts at: 0 231 1380
Size: 231 1149
SbfI CC_TGCA'GG
Cuts at: 0 767 1380
Size: 767 613
ScaI AGT'ACT
Cuts at: 0 298 1380
Size: 298 1082
SexAI A'CCwGG_T
Cuts at: 0 503 560 1380
Size: 503 57 820
Fragments arranged by size:
820 503 57
SfcI C'TryA_G
Cuts at: 0 186 763 1380
Size: 186 577 617
Fragments arranged by size:
617 577 186
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 326 418 1380
Size: 326 92 962
Fragments arranged by size:
962 326 92
SmaI CCC'GGG
Cuts at: 0 10 1380
Size: 10 1370
SmlI C'TyrA_G
Cuts at: 0 1292 1380
Size: 1292 88
StuI AGG'CCT
Cuts at: 0 412 769 1071 1380
Size: 412 357 302 309
Fragments arranged by size:
412 357 309 302
StyI C'CwwG_G
Cuts at: 0 324 407 770 1380
Size: 324 83 363 610
Fragments arranged by size:
610 363 324 83
TatI w'GTAC_w
Cuts at: 0 296 1380
Size: 296 1084
Tth111I GACn'n_nGTC
Cuts at: 0 505 1380
Size: 505 875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 379 558 652 1380
Size: 379 179 94 728
Fragments arranged by size:
728 379 179 94
XmaI C'CCGG_G
Cuts at: 0 8 1380
Size: 8 1372
ZraI GAC'GTC
Cuts at: 0 1065 1380
Size: 1065 315
Enzymes that do cut:
AatII AccI AcuI AlfI AlwNI ApaI ApaLI ApoI
AvaI BanII BbvCI BglI BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BsrBI BsrFI BssSI BstAPI
BstYI Bsu36I BtsI ClaI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI MscI MslI MspA1I NaeI NgoMIV
NspI PpuMI PshAI PspOMI PstI PvuII SbfI ScaI
SexAI SfcI SfiI SmaI SmlI StuI StyI TatI
Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbsI BcgI BciVI BclI BfrBI BglII
BmtI BplI BsaI BsaAI BsaBI BsaXI BsiWI BsmBI
BspEI BspHI BspMI BsrDI BsrGI BssHII BstBI BstEII
BstXI BstZ17I BtgI BtgZI CspCI DraI DraIII DrdI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TstI XbaI XhoI XmnI