Restriction map for
pF1KB9874
(Linear) (Six-base) MAPSORT of: KIBB9874.seq Check: 4571 from: 1 to: 1545
KIBB9874.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 231 1545
Size: 231 1314
AccI GT'mk_AC
Cuts at: 0 272 1545
Size: 272 1273
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 844 1139 1522 1545
Size: 143 701 295 383 23
Fragments arranged by size:
701 383 295 143 23
AleI CACnn'nnGTG
Cuts at: 0 469 883 1545
Size: 469 414 662
Fragments arranged by size:
662 469 414
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1350 1382 1545
Size: 1350 32 163
Fragments arranged by size:
1350 163 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1457 1545
Size: 1457 88
ApoI r'AATT_y
Cuts at: 0 686 760 816 900 1545
Size: 686 74 56 84 645
Fragments arranged by size:
686 645 84 74 56
AvrII C'CTAG_G
Cuts at: 0 376 696 1545
Size: 376 320 849
Fragments arranged by size:
849 376 320
BbsI GAAGACnn'nnnn_
Cuts at: 0 248 1545
Size: 248 1297
BfrBI ATG'CAT
Cuts at: 0 286 959 1545
Size: 286 673 586
Fragments arranged by size:
673 586 286
BglII A'GATC_T
Cuts at: 0 68 1533 1545
Size: 68 1465 12
Fragments arranged by size:
1465 68 12
Bme1580I G_kGCm'C
Cuts at: 0 1351 1545
Size: 1351 194
BmrI ACTGGGnnnn_n'
Cuts at: 0 240 1545
Size: 240 1305
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 373 436 1545
Size: 52 321 63 1109
Fragments arranged by size:
1109 321 63 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 591 1312 1545
Size: 328 263 721 233
Fragments arranged by size:
721 328 263 233
BsaAI yAC'GTr
Cuts at: 0 1488 1545
Size: 1488 57
BsaBI GATnn'nnATC
Cuts at: 0 851 1545
Size: 851 694
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 34 64 349 379 1350 1380 1545
Size: 34 30 285 30 971 30 165
Fragments arranged by size:
971 285 165 34 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 370 402 1545
Size: 370 32 1143
Fragments arranged by size:
1143 370 32
BsiHKAI G_wGCw'C
Cuts at: 0 808 1545
Size: 808 737
BsmI GAATG_Cn'
Cuts at: 0 104 1545
Size: 104 1441
Bsp1286I G_dGCh'C
Cuts at: 0 808 1351 1545
Size: 808 543 194
Fragments arranged by size:
808 543 194
BspHI T'CATG_A
Cuts at: 0 512 1545
Size: 512 1033
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 231 1545
Size: 231 1314
BsrBI CCG'CTC
Cuts at: 0 1163 1545
Size: 1163 382
BstBI TT'CG_AA
Cuts at: 0 96 1545
Size: 96 1449
BstEII G'GTnAC_C
Cuts at: 0 1090 1156 1307 1321 1545
Size: 1090 66 151 14 224
Fragments arranged by size:
1090 224 151 66 14
BstYI r'GATC_y
Cuts at: 0 68 1533 1545
Size: 68 1465 12
Fragments arranged by size:
1465 68 12
BtgI C'CryG_G
Cuts at: 0 567 1255 1545
Size: 567 688 290
Fragments arranged by size:
688 567 290
BtsI GCAGTG_nn'
Cuts at: 0 878 890 1406 1545
Size: 878 12 516 139
Fragments arranged by size:
878 516 139 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 42 77 1545
Size: 42 35 1468
Fragments arranged by size:
1468 42 35
DraI TTT'AAA
Cuts at: 0 705 899 1545
Size: 705 194 646
Fragments arranged by size:
705 646 194
DraIII CAC_nnn'GTG
Cuts at: 0 1491 1545
Size: 1491 54
DrdI GACnn_nn'nnGTC
Cuts at: 0 601 1545
Size: 601 944
EaeI y'GGCC_r
Cuts at: 0 570 1545
Size: 570 975
EarI CTCTTCn'nnn_
Cuts at: 0 308 1545
Size: 308 1237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 143 373 436 844 1139 1522 1545
Size: 52 91 230 63 408 295 383 23
Fragments arranged by size:
408 383 295 230 91 63 52 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1316 1373 1545
Size: 1316 57 172
Fragments arranged by size:
1316 172 57
EcoRI G'AATT_C
Cuts at: 0 760 1545
Size: 760 785
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 162 318 350 1372 1404 1545
Size: 130 32 156 32 1022 32 141
Fragments arranged by size:
1022 156 141 130 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 486 1545
Size: 486 1059
MscI TGG'CCA
Cuts at: 0 572 1545
Size: 572 973
MslI CAynn'nnrTG
Cuts at: 0 469 883 1545
Size: 469 414 662
Fragments arranged by size:
662 469 414
MspA1I CmG'CkG
Cuts at: 0 121 414 1257 1545
Size: 121 293 843 288
Fragments arranged by size:
843 293 288 121
NcoI C'CATG_G
Cuts at: 0 567 1545
Size: 567 978
NsiI A_TGCA'T
Cuts at: 0 288 961 1545
Size: 288 673 584
Fragments arranged by size:
673 584 288
PflMI CCAn_nnn'nTGG
Cuts at: 0 1101 1545
Size: 1101 444
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1132 1164 1350 1382 1545
Size: 1132 32 186 32 163
Fragments arranged by size:
1132 186 163 32 32
PsiI TTA'TAA
Cuts at: 0 851 1545
Size: 851 694
PstI C_TGCA'G
Cuts at: 0 184 242 885 1545
Size: 184 58 643 660
Fragments arranged by size:
660 643 184 58
PvuII CAG'CTG
Cuts at: 0 121 414 1545
Size: 121 293 1131
Fragments arranged by size:
1131 293 121
SacII CC_GC'GG
Cuts at: 0 1258 1545
Size: 1258 287
ScaI AGT'ACT
Cuts at: 0 1011 1545
Size: 1011 534
SfcI C'TryA_G
Cuts at: 0 180 238 650 881 1181 1286 1545
Size: 180 58 412 231 300 105 259
Fragments arranged by size:
412 300 259 231 180 105 58
SmlI C'TyrA_G
Cuts at: 0 307 606 1327 1545
Size: 307 299 721 218
Fragments arranged by size:
721 307 299 218
SpeI A'CTAG_T
Cuts at: 0 1012 1545
Size: 1012 533
SspI AAT'ATT
Cuts at: 0 629 1070 1545
Size: 629 441 475
Fragments arranged by size:
629 475 441
StuI AGG'CCT
Cuts at: 0 205 1545
Size: 205 1340
StyI C'CwwG_G
Cuts at: 0 18 376 567 696 1545
Size: 18 358 191 129 849
Fragments arranged by size:
849 358 191 129 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 100 1545
Size: 100 1445
TatI w'GTAC_w
Cuts at: 0 981 1009 1545
Size: 981 28 536
Fragments arranged by size:
981 536 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 34 66 1477 1509 1545
Size: 34 32 1411 32 36
Fragments arranged by size:
1411 36 34 32 32
XbaI T'CTAG_A
Cuts at: 0 1150 1545
Size: 1150 395
Enzymes that do cut:
AarI AccI AcuI AleI AloI AlwNI ApoI AvrII
BbsI BfrBI BglII Bme1580I BmrI BpmI BpuEI BsaAI
BsaBI BsaXI BseRI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BstBI BstEII BstYI BtgI BtsI CspCI DraI
DraIII DrdI EaeI EarI Eco57MI EcoNI EcoRI Hin4I
MmeI MscI MslI MspA1I NcoI NsiI PflMI PpiI
PsiI PstI PvuII SacII ScaI SfcI SmlI SpeI
SspI StuI StyI TaqII TatI TstI XbaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI ApaI ApaLI AscI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BlpI BmgBI BmtI BplI Bpu10I BsaI BsaHI
BsaWI BseYI BsgI BsiEI BsiWI BsmBI BspEI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstXI BstZ17I Bsu36I
BtgZI ClaI EagI EciI EcoICRI EcoO109I EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NspI PacI PasI PciI PfoI PmeI
PmlI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII
SacI SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SphI SrfI SwaI Tth111I
XcmI XhoI XmaI XmnI ZraI